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2010 Vol.  12 No.  09
Published: 2010-09-15
EXPERT LECTURE
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
EXPERT LECTURE
681 Richard James HUNTSMAN, Noel John LOWRY, Koravangattu SANAKARAN
Non epileptic motor phenomena in the newborn
No abstract available
2010 Vol. 12 (09): 681-689 [Abstract] ( 4494 ) [HTML 1KB] [PDF 1073KB] ( 1715 )
CLINICAL RESEARCH
690 LIU Cui-Qing, XIA Yao-Fang, YUAN Yu-Xiao, LI Li, QIU Xiang-Li
Effects of selective head cooling with mild hypothermia on serum levels of caspase-3 and IL-18 in neonates with hypoxic-ischemic encephalopathy

OBJECTIVE: The study examined the changes of serum caspase-3 and IL-8 levels following selective head cooling with mild hypothermia (SHC) treatment in neonates with hypoxic-ischemic encephalopathy (HIE) in order to explore the mechanism of neuroprotection of SHC against HIE. METHODS: Thirty-three neonates with moderate or severe HIE were randomly assigned to two groups: SHC treatment (n=16）and conventional treatment (n=17). Serum levels of caspase-3 and IL-18 were measured using ELISA before treatment and 24 hrs, 48 hrs, 72 hrs and 5 days after treatment. RESULTS: Serum caspase-3 levels in the SHC group decreased 24 and 48 hrs after treatment (3.8±1.9 and 2.6±1.2 ng/mL, respectively) compared with 6.1±2.3 ng/mL at 24 hrs and 7.2±3.1 ng/mL at 48 hrs in the conventional treatment group (P<0.05). Serum IL18 levels in the SHC group decreased 24 hrs, 48 hrs and 72 hrs after treatment (119±30, 76±33 and 71±40 ng/mL, respectively) compared with those in the conventional treatment group (138±28 ng/mL at 24 hrs, 156±60 ng/mL at 48 hrs and 182±54 ng/mL at 72 hrs; P<0.01). CONCLUSIONS: SHC treatment can inhibit the release of caspase-3 and the expression of IL-18 in neonates with moderate or severe HIE. This may contribute to the neuroprotection of SHC against HIE.［Chin J Contemp Pediatr, 2010, 12 (9):690-692］
2010 Vol. 12 (09): 690-692 [Abstract] ( 5702 ) [HTML 1KB] [PDF 925KB] ( 1717 )
693 HU Xiao-Yan, ZHOU Yu-Xin, XU Song-Zhou, LIN Yue-Yu
Effects of probiotics on feeding intolerance in low birth weight premature infants
OBJECTIVE: To study the efficacy and safety of probiotics for the prevention of feeding intolerance in low birth weight (LBW) premature infants. METHODS: Sixty eligible LBW premature infants were randomly divided into probiotics and conventional treatment groups (n=30 each). Both groups received treatment of the primary disease. Additionally, the probiotics treatment group was administered with probiotics (0.25 g, twice daily). The incidence of feeding intolerance, the time to regain birth weight and to reach full enteral nutrition and the length of hospitalization were compared between the two groups. The occurrence of adverse reactions was recorded. RESULTS: The incidence of feeding intolerance in the probiotics treatment group was lower than that in the conventional treatment group (4% vs 14%; P<0.01). The time to regain birth weight (6.8±1.2 days vs 7.7±1.6 days; P<0.05) and the time to reach full enteral nutrition (8.0±1.4 days vs 9.0±2.0 days; P<0.05) in the probiotics treatment group were shorter than those in the conventional treatment group. No adverse reactions were observed in the probiotics treatment group. CONCLUSIONS: Probiotics can reduce the incidence of feeding intolerance in LBW premature infants, can promote weight gain and shorten the time to reach full enteral nutrition. The application of probiotics appears to be safe in LBW premature infants.［Chin J Contemp Pediatr, 2010, 12 (9):693-695］
2010 Vol. 12 (09): 693-695 [Abstract] ( 6379 ) [HTML 1KB] [PDF 923KB] ( 2208 )
696 LI Qiu-Ping, WANG Zong-Hua, CHEN Yao-Qin, CHEN Jia, HUANG Jie-Ting, WANG Zi-Zhen, FENG Zhi-Chun
Bedside treatment of retinopathy of premeturity by laser photocoagulation
OBJECTIVE: To study the efficacy of bedside treatment by laser photocoagulation for retinopathy of prematurity (ROP) in preterm infants hospitalized in the Neonatal Intensive Care Unit (NICU). METHODS: The clinical data of　30 cases of ROP who underwent peripheral laser ablation on bedside in the NICU from March to August 2009 were studied retrospectively. RESULTS: A total of 59 eyes from 30 patients received the laser therapy, with a total cure rate of 95%. According to the International Classification of ROP, 26 eyes of 13 infants had zone 1 disease, and 33 eyes of 17 infants had zone 2 disease. The birth gestational age and birth weight as well as corrected gestational age and corrected weight at operation in the zone 1 disease group were significantly lower than those in the zone 2 disease group. The number of laser spots in the zone 1 disease group was significantly higher than that in the zone 2 disease group. The cure rate in the zone 2 disease group (100%) was significantly higher than that in the zone 1 disease group (88%). CONCLUSIONS: Laser retinal photocoagulation on bedside in the NICU is effective for both zone 1 and zone 2 ROP. As compared with the infants with zone 2 disease, the infants with zone 1 disease may have a poor outcome. ［Chin J Contemp Pediatr, 2010, 12 (9):696-699］
2010 Vol. 12 (09): 696-699 [Abstract] ( 5022 ) [HTML 1KB] [PDF 983KB] ( 1583 )
700 MAI Jing-Yun, ZHU Min-Li, CHEN Chun, HE Xiao-Liang, LIN Zhen-Lang
Clinical characteristics of neonatal Klebsiella pneumoniae sepsis and the antibiotic sensitivity pattern of strains
OBJECTIVE: To study the clinical characteristics of neonatal sepsis caused by Klebsiella pneumoniae and the antibiotic sensitivity pattern of Klebsiella pneumoniae strains.MethodsThe clinical data of 42 cases of neonatal sepsis caused by Klebsiella pneumoniae from January, 2000 to August, 2009 were retrospectively studied. RESULTS: The clinical presentations were non-specific, including fever or hypothermia, tachypnea, apnea and feeding intolerance. C-reactive protein (CRP) level increased in 95% of the cases. The mortality was 21%. In neonates with early onset sepsis, Klebsiella pneumoniae strains were sensitive to amoxicillin/clavulanic-acid, piperacillin/tazobactam, cefoxitin, imipenem, cefoperazone/and sulbactam. In neonates with late onset sepsis, the sensitive antibiotics of Klebsiella pneumoniae strains were less, including cefoxitin, piperacillin/tazobactam and imipenem. Klebsiella pneumoniae strains were not sensitive to penicillins and cephalosporins in either neonates with early onset sepsis or late onset sepsis. The extended spectrum β-lactamases (ESBLs)-producing strains were found in 92% of the cases. The neonates with late onset sepsis presented a higher prevalence of ESBLs-producing strains than those with early onset sepsis (100% vs 70%; P<0.05). CONCLUSIONS: The clinical manifestations of neonatal sepsis caused by Klebsiella pneumoniae are usually non-specific. CRP detection is valuable for early diagnosis of sepsis. There are differences in the antibiotic sensitivity of strains between the neonates with early onset and late onset Klebsiella pneumoniae sepsis.［Chin J Contemp Pediatr, 2010, 12 (9):700-703］
2010 Vol. 12 (09): 700-703 [Abstract] ( 8687 ) [HTML 1KB] [PDF 936KB] ( 2184 )
704 CHEN Yan-Zhao, WEN Fei-Qiu, ZHOU Ke-Ying, YANG Chun-He, ZHANG Wei, LI Ning
Clinical features of various subtypes of attention deficit hyperactivity disorders in children
OBJECTIVE: To study the features of various subtypes of attention deficit hyperactivity disorders (ADHD) in children. METHODS: Sex composition, risk factors, comorbidities, intelligence quotient and behavioral problems were investigated in 175 children with ADHD who met the Diagnostic Statistical Manual of Mental Disorder Criteria (DSM-IV). The children were classified into three groups: ADHD predominantly inattentive (ADHD-I, n=82), ADHD predominantly hyperactive-impulsive (ADHD-HI, n=24) and ADHD combined type (ADHD-C, n=69). RESULTS: There were no significant differences in the sex composition among the three groups. The rates of birth abnormality in the ADHD-I and the ADHD-C groups were higher than those in the ADHD-HI group. Negative parenting practices were noted more frequently in the ADHD-HI and the ADHD-C groups than the ADHD-I group. There were no significant differences in the performance intelligence quotient (PIQ), verbal intelligence quotient (VIQ) and full intelligence quotient (FIQ) among the three groups. However, the incidence of imbalance between VIQ and PIQ in the ADHD-I group was higher than the other two groups. The rate of comorbidities with oppositional defiant disorder (ODD) and tic disorder (TD) in the ADHD-C and the ADHD-HI groups was higher than that in the ADHD-I group. Both the ADHD-I and the ADHD-C groups had a higher rate of comorbidities with learning disorder (LD) than the ADHD-HI group. The implusive/hyperactive and conduct problems were more severe and the hyperactivity index was higher in the ADHD-C and the ADHD-HI groups than those in the ADHD-I group, while the learning difficulties in the ADHD-I group were the most severe. CONCLUSIONS: The children with ADHD-C or ADHD-HI have higher incidences of comorbidities with ODD and TD than those with ADHD-I who the learning difficulties and the imbalance between VIQ and PIQ are more severe.［Chin J Contemp Pediatr, 2010, 12 (9):704-708］
2010 Vol. 12 (09): 704-708 [Abstract] ( 6089 ) [HTML 1KB] [PDF 950KB] ( 2179 )
709 YANG Zhi-Quan, YUAN Xian-Rui, ZHOU Yan-Hong, LI Xue-Jun, WU Jun, LIU Hong-Wei, XI Jian, LIU Qing, HOU Yong-Hong
Surgical outcomes of pediatric symptomatic epilepsy and their influencing factors
OBJECTIVE: To investigate the surgical outcomes of pediatric symptomatic epilepsy and the influencing factors for postoperative outcomes. METHODS: A cohort of 48 children with symptomatic epilepsy received surgical treatment from October 2004 to September 2008. The surgical outcomes were followed up. RESULTS: A 27.3 months (range 12-51 months) follow-up was performed in 43 cases. Engel classification for evaluating postoperative epileptic outcomes showed that class I in 32 cases (74％), class II in 4 cases (9％), class III in 4 cases (9％) and class IV in 3 cases (7%). Preoperative seizure frequency is an independent predictor of postoperative epileptic outcomes (P<0.05). CONCLUSIONS: Operative treatment can lead to a favorable result in children with symptomatic epilepsy. Preoperative seizure frequency is an independent influencing factor for postoperative outcomes.［Chin J Contemp Pediatr, 2010, 12 (9):709-711］
2010 Vol. 12 (09): 709-711 [Abstract] ( 5304 ) [HTML 1KB] [PDF 927KB] ( 1349 )
712 HU Ying-Hui, JIAO An-Xia
Clinical features of endogenous bronchial foreign bodies and application of bronchoscopy in children
OBJECTIVE: To study the clinical features of endogenous bronchial foreign bodies and the value of bronchoscopy in children. METHODS: One hundred and six children who presented lobe or lung segment atelectasis by the chest X-ray and bronchial foreign body inhalation was excluded by bronchoscopy were enrolled. The original diseases included Mycoplasma pneumonia (n=62), endobronchial tuberculosis (n=24), bronchial pneumonia (n=16), nephrotic syndrome (n=2), laryngotracheal bronchitis (n=1) and bronchiolitis (n=1). On the basis of conventional treatment of the original diseases, bronchoscopy was performed in the children. Eighty children with bronchial foreign body inhalation severed as the control group. RESULTS: Bronchoscopy showed the properties of endogenous foreign bodies: mucus emboli in 77 cases, cheese substances in 24 cases, dendritic white membrane in 4 cases, thrombosis in 1 case, and flaky pseudomembrane in 1 case. Hyperplasia of granulation tissue was seen in 25 cases. Of the 25 cases, endobronchial tuberculosis as the original disease was found in 22 cases. Mediastinal emphysema and pneumothorax occurred in 4 cases in the control group, but none in the endogenous foreign bodies group. The number of bronchoalveolar lavage by bronchoscopy in the endogenous foreign bodies group was significantly higher than that in the control group. CONCLUSIONS: Bronchoscopy is valuable in the diagnosis and treatment of endogenous bronchial foreign bodies.［Chin J Contemp Pediatr, 2010, 12 (9):712-714］
2010 Vol. 12 (09): 712-714 [Abstract] ( 5335 ) [HTML 1KB] [PDF 919KB] ( 1492 )
715 FENG Hui, XIANG Li, SHEN Kun-Ling
Dynamical changes of lung function and immunologic markers in asthmatic children receiving specific immunotherapy with standardized house dust mite extract
OBJECTIVE: To study the effects of specific immunotherapy with standardized house dust mite extract on the lung function and immunologic markers in children with asthma. METHODS: Thirty-two children with mild to moderate asthma who were allergic to house dust mite were assigned to two groups: an immunotherapy group that received subcutaneous specific immunotherapy with standardized house dust mite extract beside glucocorticoids treatment (n=15) and a control group that received glucocorticoids treatment alone (n=17). Lung function was determined before treatment and 3, 6, 12, 18 and 24 months after treatment. The frequency of asthma attacks was observed before treatment and 12 and 24 months after treatment. Serum immunological markers, including total IgE, specific IgE and specific IgG4 against house dust mite (Dermatophagoides pteronyssinus, Der P), ECP, IL-10, IL-4, and IFN-γ, were measured before treatment and 12 and 24 months after treatment. RESULTS: The parameters of lung function were kept at the normal level or near normal level after treatment in the immunotherapy group. Compared with before treatment, the percentage of patients without asthma attacks increased significantly after treatment in the immunotherapy group (P<0.05) and increased more significantly with a prolonged treatment time （P<0.05). The level of serum sIgG4 against Der P increased significantly throughout the period of the immunotherapy treatment compared with before treatment (P<0.01). There were no significant differences in the levels of serum total IgE, sIgE against Der P, ECP, IL-10, IL-4 and IFN-γ before and after treatment in the immunotherapy group. There were no significant differences in all the parameters measured before and after treatment in the control group. CONCLUSIONS: Subcutaneous specific immunotherapy with standardized house dust mite extract for two years decreases the frequency of asthma attacks and the lung functions are kept in a normal level in children with asthma. The role of the specific immunotherapy may attribute to the increased level of serum sIgG4 against Der P.［Chin J Contemp Pediatr, 2010, 12 (9):715-719］
2010 Vol. 12 (09): 715-719 [Abstract] ( 6036 ) [HTML 1KB] [PDF 949KB] ( 1596 )
720 SHU Ye, TANG Jian-Ping, ZHANG Dan, ZENG Ying-Hong, ZHOU Bin
Investigation of allergens in 3 504 children with allergic diseases
OBJECTIVE: To investigate the allergens of various allergic diseases in children. METHODS: Serum levels of Fx5E, Phadiatop and specific IgE were measured by the UniCAP100 System in 3 504 children with allergic diseases. RESULTS: The positive rate of aeroallergens was obviously higher than that of food allergens in children with allergic rhinitis, allergic conjunctivitis, asthma and papular urticaria. In contrast, the positive rate of food allergens was obviously higher than that of aeroallergens in children with Henoch-Schonlein purpura and digestive diseases. The serum specific IgE level of aeroallergens was higher than that of food allergens. The dust and mite specific IgE levels reached to grade 6, while the food allergen specific IgE levels were lower than grade 3. CONCLUSIONS: Aaeroallergens or food allergens vary remarkably in different allergic diseases in children. The level of specific IgE of aeroallergens is higher than that of food allergens.［Chin J Contemp Pediatr, 2010, 12 (9):720-722］
2010 Vol. 12 (09): 720-722 [Abstract] ( 5503 ) [HTML 1KB] [PDF 974KB] ( 2438 )
723 WEN Chuan, WANG Cheng, LI Wen, WU Li-Jia, XU Yi, LIN Ping, LUO Hai-Yan, LI Ming-Xiang, CAO Min-Jing, XIE Zhen-Wu
Clinical analysis of vasovagal syncope in children
OBJECTIVE: To study the changes in clinical features of vasovagal syncope (VVS) in children. METHODS: From January 2000 to September 2009, 841 children with unexplained syncope or prodromata were enrolled. They were assigned to two groups according to the period of visiting hospital: group A (from January 2000 to December 2004, n=129) and group B (from January 2005 to September 2009, n=712). They were assigned to three age groups: 4-6 years old, 7-10 years old and 11-18 years old. A head-up tilt table test (HUTT) was performed on all the subjects. RESULTS: The total positive rate of HUTT was 45.3% (381/841). Compared with that in group A, the positive rate of HUTT in group B increased significantly (47.5% vs 33.3%; P<0.05). The positive rate of HUTT in female children was significantly higher than that in male children (49.3% vs 37.9%; P<0.05). The positive rate of HUTT increased with age and it was the highest in children at age of 11-18 years (49.2%), followed by in children at age of 7-10 years (44.1%) and 4-6 years (37.1%) (P<0.05). The children at age of 7-10 years and 11-18 years from group B showed significantly higher positive rate of HUTT than those from group A (46.2% vs 27.8%; 54.0% vs 32.6%, P＜0.05). Vasodepressor type was the most common response type (70.9%) shown by HUTT compared with mixed type (25.5%) and cardioinhibitory type (3.6%) (P＜0.05). The proportion of children with vasodepressor response type in group B was significantly higher than that in group A (72.5% vs 58.1%; P＜0.05). CONCLUSIONS: There were obvious changes in the prevalence of VVS and response types before and after five years, suggesting that the development of VVS may be attributed to many factors, such as social factors, mental factor and life style.［Chin J Contemp Pediatr, 2010, 12 (9):723-725］
2010 Vol. 12 (09): 723-725 [Abstract] ( 5051 ) [HTML 1KB] [PDF 926KB] ( 1588 )
726 LI Juan, HOU Ying, ZHAO Yuan, ZHANG Ze-Min, MAO Jian
Value of microbial gene 16SrRNA in the identification of antenatal infection
OBJECTIVE: To study the relationship between microbial gene 16SrRNA and intrauterine infection. METHODS: Thirty cases of single preterm birth were enrolled, including 16 cases due to premature rupture of membranes (PROM) (rupture time>18 hrs), 6 cases due to spontaneous preterm birth and 8 cases due to iatrogenic preterm birth. Ten cases of single term birth were used as the control group. Fetal membrane and placenta samples were obtained. Amniotic fluid, blood from cord or newborn babies as well as gastric fluid and tracheal secretions from infants with mechanical ventilation were also obtained. The histological features of placenta and fetal membranes were observed. Polymerase chain reaction (PCR) was used to detect the presence of microbial 16SrRNA and ureaplasma urealyticum (UU) in placenta, fetal membranes and other samples. RESULTS: Twenty-one (70%) cases were diagnosed as chorioamnionitis, characterized by neutrophil infiltration in fetal membrane and placenta tissues, especially in fetal membranes. Chorioamnionitis was most frequent in babies whose gestational age less than 32 weeks or birth weight lower than 1 500 g. Positive 16SrRNA gene was found in 12 cases, and positive UU gene in 10 cases in the preterm birth group. Neither 16SrRNA nor UU gene was detected in the control group. The PROM preterm babies developed more frequent infection than the babies premature born due to other causes, but there were no statistically significant differences in the incidence of infection. CONCLUSIONS: Chorioamnionitis may be the major cause of PROM and premature birth. The detection of microbial genes is valuable in identification of intrauterine infection.［Chin J Contemp Pediatr, 2010, 12 (9):726-729］
2010 Vol. 12 (09): 726-729 [Abstract] ( 5244 ) [HTML 1KB] [PDF 965KB] ( 1723 )
730 LU Wen-Li, WANG Wei, WANG De-Fen, XIAO Yuan, HUANG Xiao-Ping, DONG Zhi-Lan
Relationship between the polymorphism of growth hormone receptor Ex3 and the efficacy of rhGH treatment in children with idiopathic short stature
OBJECTIVE: To assess the influence of growth hormone receptor (GHR) Ex3 genotype on the short-term response to recombinant human growth hormone (rhGH) therapy in children with idiopathic short stature (ISS). METHODS: Thirty prepubertal children with ISS receiving rhGH treatment ［0.116±0.02 IU/(kg/d)］ were randomly recruited. The GHR Ex3 locus was genotyped using a PCR multiplex assay. The growth data including growth velocity, height SDS for chronological age (HtSDSCA), height SDS for bone age (HtSDSBA) and predict final height were compared in children with different GHR genotypes 6 months after rhGH treatment. RESULTS: After 6 months of rhGH treatment, the children with ISS carrying d3/d3 alleles showed a significantly higher increment in growth velocity than those carrying fl/fl alleles (6.3±1.6 cm/year vs 3.4±0.5 cm/year; P<0.05). CONCLUSIONS: The polymorphism in GHR Ex3 is associated with the responsiveness to rhGH treatment, showing that the growth velocity in ISS children with d3/d3 genotype is significantly higher than those with fl/fl genotype.［Chin J Contemp Pediatr, 2010, 12 (9):730-733］
2010 Vol. 12 (09): 730-733 [Abstract] ( 5779 ) [HTML 1KB] [PDF 954KB] ( 1603 )
734 ZHU Di-Ling, YANG Wen-Xu, YANG Hui-Ming
Meta analysis of lactic acid bacteria as probiotics for the primary prevention of infantile eczema
OBJECTIVE: To determine whether lactic acid bacteria as probiotics is efficacious in the primary prevention of infantile eczema or atopic eczema. METHODS: For this meta analysis of randomized controlled trials (RCT) describing the efficacy of probiotics in infants with eczema or atopic eczema at ages of ≤2 years, a comprehensive search in the databases was performed up to January 2010. Three reviewers independently evaluated the studies for methodological qualities. RevMan 5.0.2 software was used for meta analysis. RESULTS: Twelve RCTs on the preventive effects of lactic acid bacteria as probiotics on infantile eczema were included, and 7 of the 12 RCTs reported the preventive effect of lactic acid bacteria on atopic eczema. The meta analysis showed that there was an overall significant reduction in infantile eczema and atopic eczema favoring lactic acid bacteria compared with placebo. The relative risk (RR) ratios for eczema and atopic eczema were 0.80 (95%CI: 0.70-0.90; P<0.01) and 0.78 (95%CI: 0.64-0.97; P<0.01), respectively. Lactic acid bacteria combined with other probiotics decreased significantly the incidence of eczema, with a RR ratio of 0.79 (95%CI: 0.68-0.93; P<0.01). The use of lactic acid bacteria alone did not result in a reduction in the incidence of eczema, with a RR ratio of 0.85 (95%CI: 0.69-1.05; P>0.05). CONCLUSIONS: The data from this meta analysis suggest that lactic acid probiotics combined with other probiotics play a role in the prevention of infantile eczema. There is insufficient evidence to recommend single use of lactic acid bacteria for prevention of eczema. Further studies are required to determine whether the findings are reproducible.［Chin J Contemp Pediatr, 2010, 12 (9):734-739］
2010 Vol. 12 (09): 734-739 [Abstract] ( 7627 ) [HTML 1KB] [PDF 1916KB] ( 2732 )
EXPERIMENTAL RESEARCH
740 XIE Kun-Xia, XIAO Yan-Feng, XU Er-Di, YIN Chun-Yan, YI Xiao-Qing, CHANG Ming
Effect of early high fat diet on pancreatic β cellularity and insulin sensibility in young rats
OBJECTIVE: To study the effects of early high fat diet on sugar metaboliam, insulin sensibility and pancreatic β cellularity in young rats. METHODS: Sixty male weaned young rats were randomly fed with high fat diet (high fat group) and normal diet (control group). The body weight, viscus fattiness and fasting plasma glucose (FPG) were measured after 3, 6 and 9 weeks. Serum insulin level was measured with radioimmunoassay. The ultrastructure of pancreas was observed under an electricmicroscope. RESULTS: The high fat group had significantly higher body weight and visceral fat weight than the control group after 3 weeks. There were no significant differences in the FPG level between the two groups at all time points. The levels of fasting insulin and HOMAIR in the high fat group were significantly higher than those in the control group after 3, 6 and 9 weeks (P<0.01). Dilation of rough endoplasmic reticulum and mild swelling of mitochondria of islet β-cells were observed in the high fat group after 6 weeks. CONCLUSIONS: Early high fat diet may induce a reduction in insulin sensitivity and produce insulin resistance in young rats. Endoplasmic reticulum expansion in β-cells may be an early sign of β-cell damage due to obesity.［Chin J Contemp Pediatr, 2010, 12 (9):740-743］
2010 Vol. 12 (09): 740-743 [Abstract] ( 5162 ) [HTML 1KB] [PDF 1201KB] ( 1437 )
744 HUA Wang, JIANG Jian-Bin, RONG Xing, WU Rong-Zhou, QIU Hui-Xian, ZHANG Yuan-Hai, CHEN Qi
Expression of cystathionine-γ-lyase/hydrogen sulfide pathway in CVB3-induced myocarditis in mice
OBJECTIVE: Previous studies have shown that hydrogen sulfide (H2S) plays key roles in a number of biological processes, including vasorelaxation, inflammation, apoptosis, ischemia/reperfusion and oxidative stress, which are involved in the pathogenesis of myocarditis. This study aimed to examine the expression of cystathionine-γ-lyase(CSE)/H2S pathway in mice with viral myocarditis. METHODS: Six-week-old inbred male mice were randomly assigned to control (n=25) and myocarditis group (n=30). The myocarditis and the control groups were inoculated intraperitoneally with 0.1 mL 10-5.69TCID50/mL CVB3 or vehicle (PBS) alone respectively. Ten mice were sacrificed 4 and 10 days after injection. Blood and heart specimens were harvested for measuring the content of serum H2S and the H2S production rates in cardiac tissues. Heart sections were stained with hematoxylin and eosin. Immunohistochemisty was used to detect the CSE protein expression in the heart. RESULTS: In the myocarditis group, the serum H2S content and H2S production rates in cardiac tissues were significantly higher than those in the control group 4 and 10 days after injection (P<0.05). The expression of CSE protein in the heart in the myocarditis group was also significantly higher than that in the control group (P<0.05). CONCLUSIONS: CSE and its downstream production H2S increase in mice with acute viral myocarditis. The increased expression of CSE/H2S pathway might be involved in the pathogenesis of viral myocarditis.［Chin J Contemp Pediatr, 2010, 12 (9):744-748］
2010 Vol. 12 (09): 744-748 [Abstract] ( 6181 ) [HTML 1KB] [PDF 1212KB] ( 2211 )
CLINICAL EXPERIENCE
749 OU Yang-Wen-Xian, YOU Jie-Yu, CHEN Chang-Bin, CHEN Zhi-Yong, LUO Yan-Hong, ZHAO Hong-Mei, TANG Shuo, LIU Li
Lactulose use in bowel preparation before pediatric colonoscopy

No abstract available
2010 Vol. 12 (09): 749-751 [Abstract] ( 5222 ) [HTML 1KB] [PDF 919KB] ( 1915 )
751 ZHOU Jian-Feng, YI Jun
Diagnosis and treatment of Henoch-Schonlein purpura complicated by intussusception: experience of 14 cases

No abstract available
2010 Vol. 12 (09): 751-752 [Abstract] ( 4951 ) [HTML 1KB] [PDF 898KB] ( 1982 )
753 WANG Chan, HE Xi-Yu, FENG Zhi-Chun, SUN Yong-Qiang
Nenatal screening of inherited metabolic diseases: analysis of 5 400 cases

No abstract available
2010 Vol. 12 (09): 753-755 [Abstract] ( 4111 ) [HTML 1KB] [PDF 909KB] ( 2229 )
CASE REPORT
756 LU Shan, LIU Li-Juan, SUI Jing, WANG Xue-Mei
Bodily symptoms associated with mental disorders in two children

No abstract available
2010 Vol. 12 (09): 756-757 [Abstract] ( 3985 ) [HTML 1KB] [PDF 898KB] ( 1322 )
REVIEW
758 CHEN Hui, CHENG Yan, ZHANG Tong-Yuan
Mechanism of airway hyperresponsiveness in bronchial asthma

No abstract available
2010 Vol. 12 (09): 758-761 [Abstract] ( 5688 ) [HTML 1KB] [PDF 923KB] ( 3032 )
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