目的 探讨趋化因子受体3(CXCR3)及γ干扰素诱导蛋白-10(IP-10)在毛细支气管炎(简称毛支)患儿外周血中的表达及临床意义.方法 随机选取毛支住院患儿55例,按有无过敏因素分为毛支Ⅰ组(有过敏因素)和毛支Ⅱ组(无过敏因素);同期住院的外科非感染患儿28例作为对照组.采用流式细胞术检测3组患儿外周血CD4+、CD8+淋巴细胞表面CXCR3(表面分子标记为CD183)的表达,ELISA 法测定血清中其配体IP-10的水平.结果 毛支Ⅰ组和毛支Ⅱ组外周血CD4+ T细胞表面CD183+细胞的表达及CD8+ T细胞表面CD183+细胞的表达均高于对照组(P<0.05),且毛支Ⅰ组高于毛支Ⅱ组(P<0.05);毛支Ⅰ组及毛支Ⅱ组患儿血清IP-10的浓度高于对照组(P<0.05),毛支Ⅰ组与毛支Ⅱ组比较差异无统计学意义.结论 CXCR3及IP-10参与了毛支的发病过程,且CXCR3与过敏因素有关.
Abstract
Objective To study the roles of chemokine receptor 3 (CXCR3) on lymphocytes and interferon-γ-inducible protein-10 (IP-10) of peripheral blood in childhood bronchiolitis. Methods Fifty-five children with bronchiolitis were classified into Group I (with allergic factors) and Group II (without allergic factors). Twenty-eight children with noninfectious diseases were enrolled randomly as the control group. The expression of CXCR3 (CD183 as its molecular marker) on lymphocytes of peripheral blood was detected by flow cytometry. Serum IP-10 level was measured using ELISA. Results The expression of CD183+ cells on CD4+ and CD8+ lymphocytes in peripheral blood in children with bronchiolitis from both Group I and Group II was significantly higher than that in the control group (P<0.05), and Group I had higher expression of CD183+ cells on CD4+ and CD8+ lymphocytes than Group II (P<0.05).Serum IP-10 levels in Group I and Group II were significantly higher than those in the control group (P<0.05). However, there was no significant difference in serum IP-10 levels between Group I and Group II. Conclusions CXCR3 and IP-10 are involved in the pathogenesis of bronchiolitis, and CXCR3 is associated with allergic factors.
关键词
毛细支气管炎 /
趋化因子受体3 /
γ干扰素诱导蛋白-10 /
儿童
Key words
Bronchiolitis /
Chemokine receptor 3 /
Interferon-γ-inducible protein-10 /
Child
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参考文献
[1] 胡亚美, 江载芳. 诸福棠实用儿科学[M]. 第7 版. 北京: 人民卫生出版社, 2002: 1175-1201.
[2] 邓华,符州. 毛细支气管炎后支气管哮喘发生的相关因素[J]. 实用儿科临床杂志, 2009, 24(4): 274-276.
[3] 朱鸿玲, 赵泽飞, 方瑾, 等. CXC趋化因子受体３在甲状腺乳头状癌的表达及临床意义[J]. 上海医学, 2012, 35(1): 41-43.
[4] 祝先进, 宋艳芳, 曹颖平, 等. 慢性乙型肝炎患者外周血趋化因子CXCL10和CXCL11的表达及意义[J]. 临床肝胆病杂志, 2011, 27(8): 804-806.
[5] 王海梁. 趋化因子CXC亚族及其受体CXCR3在肝移植急性排斥反应中的作用[D]. 第二军医大学学位论文, 2008.
[6] 赵之, 李芳. 趋化因子及趋化因子受体3在自身免疫疾病中的作用[J]. 中国微生态学杂志, 2010, 22(10): 958-960.
[7] 吴良霞, 吴珉, 林晓亮, 等. 哮喘小鼠肺组织中CXC趋化因子受体3和其配体干扰素-γ诱导蛋白10的表达及意义[J]. 实用儿科临床杂志, 2009, 24 (16): 1238-1240.
[8] 邹丽萍, 张松林. 毛细支气管炎患儿ICAM-1和RANTES检测的意义[J]. 中国当代儿科杂志, 2010, 12 (3): 181-183.
[9] Kim HH, Lee MH, Lee JS. Eosinophil cationic p rotein and che2 mokines in nasopharyngeal secretions of infants with respiratory syncytial virus (RSV) bronchiolitis and non-RSV bronchiolitis [J]. J Korean Med Sci, 2007, 22(1): 37242.
[10] 郭宇, 李燕, 陈庄. 趋化因子在哮喘中的作用及研究进展[J]. 现代预防医学, 2012, 39(7): 1764-1767.
[11] 张翔, 李文倩, 李建平, 等. CXCR3 及其相关配体的研究进展[J]. 免疫学杂志,2013, 29(4): 365-368.
[12] Sauty A, Dziejman M, Taha RA, et al. The T cell-specific CXC chemokines IP-10, Mig, and I-TAC are expressed by aetivated human bronehial epithlial cells[J]. J Immunol, 1999, 162(6): 3549-3558.
[13] 张松林. 毛细支气管炎患儿T淋巴细胞亚群、ICAM-1及RANTES检测意义的研究[D]. 郑州大学学位论文, 2010.
[14] 袁瑞, 邹丽萍. 婴幼儿喘息性疾病外周血单个核细胞LFA-1及Mac-1检测意义的研究[D]. 郑州大学学位论文, 2012.
[15] 李礼. 毛细支气管炎后继发支气管哮喘相关因素分析[J].中国现代医学杂志, 2010, 20(22): 3483-3487.
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