目的探讨不同血清25(OH)D3水平哮喘患儿中血清及支气管肺泡灌洗液(BALF)中半乳糖凝集素-3(Gal-3)水平的变化及临床意义。方法选取2013年1月至2014年12月确诊为哮喘的50例患儿为哮喘组,再根据哮喘患儿血清25(OH)D3水平不同分为维生素D适当组(n=7)、不足组(n=12)和缺乏组(n=31);另选同期因气道发育畸形或支气管异物住院的20例患儿为对照组。采用ELISA法检测各组患儿血清25(OH)D3、Gal-3、TIgE及BALF中Gal-3水平。结果哮喘组患儿血清中25(OH)D3水平较对照组低(P<0.05);各组中性粒细胞、嗜酸性粒细胞及上皮细胞计数比较依次为缺乏组>不足组>适当组>对照组(P<0.05);哮喘患儿血清25(OH)D3水平与BALF中中性粒细胞、嗜酸性粒细胞及上皮细胞计数呈负相关(分别r=-0.683、-0.795及-0.670,P<0.05);哮喘患儿血清25(OH)D3水平与血清Gal-3、TIgE水平呈明显负相关(分别r=-0.759、-0.875,P<0.05)。结论哮喘患儿普遍存在维生素D缺乏或不足,25(OH)D3及Gal-3可能参与了哮喘患儿呼吸道炎性反应,与哮喘的发生、发展相关。
Abstract
Objective To investigate the changes of the levels of galectin-3(Gal-3) in serum and bronchoalveolar lavage fluid (BALF) of children with asthma whose have different serum levels of 25-hydroxyl-vitamin D3[25(OH)D3]. Methods Fifty children with asthma between January 2013 and December 2014 were enrolled as the asthma group, and they were classified into 25(OH)D3 sufficient (n=7), insufficient (n=12) and deficient subgroups (n=31) according to the serum levels of 25(OH)D3. Twenty children with abnormal airway or tracheal foreign bodies served as the control group. The levels of 25(OH)D3, Gal-3 and total IgE in serum and Gal-3 levels in BALF were measured using ELISA. Results The serum levels of 25(OH)D3 in the asthma group were lower than in the control group (P<0.05). The 25(OH)D3 deficient subgroup displayed the highest percentages of neutrophils, eosinophils and epithelial cells in BALF, followed by the 25(OH)D3 insufficient subgroup and the 25(OH)D3 sufficient subgroup (P<0.05). The percentages of neutrophils, eosinophils and epithelial cells in BALF in the three subgroups were all higher than in the control group (P<0.05). In children with asthma, serum levels of 25(OH)D3 were negatively correlated with the percentages of neutrophils, eosinophils and epithelial cells in BALF (r=-0.683, -0.795 and -0.670 respectively; P<0.05); and a negative correlation was also seen between serum 25(OH)D3 levels and serum Gal-3 and total IgE levels (r=-0.759 and -0.875 respectively; P<0.05). Conclusions The children with asthma have low serum levels of 25(OH) D3. 25(OH) D3 and Gal-3 may be involved in the airway inflammation and the development of asthma.
关键词
哮喘 /
25-羟维生素D3 /
半乳糖凝集素-3 /
儿童
Key words
Asthma /
25-Hydroxyl-vitamin D3 /
Galectin-3 /
Child
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