目的研究新疆维吾尔自治区儿童白细胞介素-4受体(IL-4R)基因多态性及IgE水平与哮喘预测指数(API)的相关性。方法选取167例API(+)、187例API(-)及203例健康婴幼儿(对照组),应用PCR聚合酶链反应和DNA测序法进行基因分型。同时ELISA法检测三组婴幼儿血清IgE水平。结果IL-4R基因Arg551Gln位点各基因型频率分布在API(+)组、API(-)组、对照组三组中差异无统计学意义(P>0.05);API(+)组血清IgE水平明显高于API(-)及对照组(P<0.01); API(+)患儿中<2岁者血清IgE水平明显低于 ≥ 2岁者(P<0.01)。结论 Arg551Gln位点多态性与API结果无相关性,而API阳性与IgE水平升高存在关联性,年龄 ≥ 2岁是API阳性的儿童IgE水平升高的风险因素。
Abstract
Objective To study the correlations of IL-4R gene polymorphism and serum IgE levels with asthma predictive index (API) in children. Methods One hundred and sixty-seven children with positive API, 187 children with negative API and 203 healthy children (control group) were enrolled. PCR and DNA sequencing were used to identify genotypes of the Arg551Gln locus in IL-4R gene. Serum IgE levels were measured using ELISA. Results There was no significant difference in the genotype frequencies of the Arg551Gln locus in IL-4R gene among the positive API, negative API and control groups. Serum IgE levels in the positive API group were significantly higher than in the negative API and control groups (P<0.01). In the positive API group, the children aged less than 2 years had significantly lower serum IgE levels than those aged over 2 years (P<0.01). Conclusions There is no correlation between the Arg551Gln polymorphism in IL-4R gene and API results. API positivity is correlated with elevated serum IgE levels. An older age (>2 years) may be a risk factor for increased serum IgE levels in children with positive API.
关键词
哮喘 /
哮喘预测指数 /
白细胞介素-4受体 /
IgE /
儿童
Key words
Asthma predictive index /
IL-4R /
IgE /
Child
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 方年新, 黄俊伟, 何小兵, 等. 支气管反应活性指数在气道高反应者哮喘的诊断价值[J]. 中山大学学报医学科学版, 2010, 31(3):428-431.
[2] Stavric K, Peova S, Trajkov D, et al. Gene polymorphisms of 22 cytokines in Macedonian children with atopic dermatitis[J]. Iran J Allergy Asthma Immunol, 2012, 11(1):37-50.
[3] Busse WW, Morgan WJ, Gergen PJ, et al. Randomized trial of omalizumab (anti-IgE) for asthma in inner-city children[J]. N Engl J Med, 2011, 364(11):1005-1015.
[4] 中华医学会儿科学会呼吸学组, 《中华儿科杂志》编辑委员会. 儿童支气管哮喘诊断与防治指南[J]. 中华儿科杂志, 2008, 46(10):745-753.
[5] Lötvall J, Akdis CA, Bacharier LB, et al. Asthma endotypes: a new approach to classification of disease entities within the asthma syndrome[J]. J Allergy Clin Immunol, 2011, 127(2): 355-360.
[6] 李亚琴, 薛海燕, 陈伟. 等. 按哮喘预测指数分组治疗在5岁以下喘息儿童中的应用[J]. 中国当代儿科杂志, 2014, 16(8): 795-799.
[7] Tanaka K, Sugiura H, Uehara M, et al. Lack of association between atopic eczema and the genetic variants of interleukin-4 and the interleukin-4 receptor α chain gene:heterogeneity of genetic backgrounds on immunoglobulin E production in atopic eczema patients[J]. Clin Exp Allergy, 2001, 31(10):1522-1527.
[8] Lee SG, Kim BS, Kim JH, et al. Gene-gene interaction between interleukin-4 and interleukin-4 receptor α in Korean children with asthma[J]. Clin Exp Allergy, 2004, 34(8):1202-1208.
[9] Busse W, Corren J, Lanier BQ, et al. Omalizumab, anti-IgE recombinant humanized monoclonal antibody, for the treatment of severe allergic asthma[J]. J Allergy Clin Immunol, 2001, 108(2):184-190.
[10] Patelis A, Janson C, Borres MP, et al. Aeroallergen and food IgE sensitization and local and systemic inflammation in asthma[J]. Allergy, 2014, 69(3):380-387.
[11] Sunyer J, Pekkanen J, Garcia-Esteban R, et al. Asthma score: predictive ability and risk factors[J]. Allergy, 2007, 62(2):142-148.
[12] 张慧芳, 潘家华, 李倩. 等. 小儿慢性咳嗽的病因分析[J]. 中国当代儿科杂志, 2012, 14(9):667-670.
[13] Kim JH, Cheong HS, Park JS, et al. A genome-wide association study of total serum and mite-specific IgEs in asthma patients[J]. PLoS One, 2013, 8(8):e71958.
基金
新疆医科大学科研创新基金资助项目(XJC2013178)。
PDF(1551 KB)
