目的探讨Toll样受体(TLR)2及TLR5基因单核苷酸多态性(SNP)分布与新生儿败血症易感性的关系。方法选取2011年5月至2014年1月确诊为败血症的新生儿114例为败血症组,另选取同期172例非感染新生儿作为对照组。采用微测序技术(SNaPshot)检测TLR2(rs5743708、rs3804099)和TLR5(rs5744105)共3个位点的基因SNP,比较两组间各等位基因频率和基因型频率的分布差异;采用多因素logistic回归模型分析TLR基因型与新生儿败血症的关系。结果 TLR2基因rs3804099(C/T)和TLR5基因rs5744105(C/G)各基因型分布在两组间差异均有统计学意义(P<0.05);上述两个位点各等位基因分布在两组间差异无统计学意义(P>0.05);TLR2基因rs5743708位点在对照组与败血症组的基因型均为GG,未发现突变。Logistic回归分析显示低胎龄(OR=3.065, P<0.05)、低出生体重(OR=3.301, P<0.05)与新生儿败血症的发生密切相关;性别(OR=1.107, P>0.05)、rs3804099(OR=0.876, P>0.05)和rs5744105(OR=0.820, P>0.05)基因多态性不是新生儿败血症发病的危险因素。结论 rs5743708、rs3804099和rs5744105可能均不是新生儿败血症的易感基因。
Abstract
Objective To study the association between single nucleotide polymorphisms(SNP) in toll-like receptors (TLR) 2 and 5 genes and the susceptibility to neonatal sepsis. Methods One hundred and fourteen newborn infants who were diagnosed with clinical sepsis (case group) between May 2011 and January 2014 and 172 newborn infants without infection(control group) were enrolled in this study. The polymorphisms of TLR2(rs5743708 and rs3804099) and TLR5(rs5744105) were analyzed using a SNaPshot multiplex reaction to compare the genotypic and allelic frequencies between two groups. The relationship between TLR genotypes and susceptibility to sepsis was analyzed by logistic regression models. Results Significant differences in genotypic frequencies of TLR2 rs3804099(C/T) and TLR5 rs5744105(C/G) were found between the two groups (P<0.05), but there was no significant difference in allelic frequencies of all the SNPs above between the two groups (P>0.05). The genotype on TLR2 rs5743708 was GG and no mutation was found in both groups. In regression models, birth weight (OR=3.065; P<0.05) and gestational age (OR=3.301; P<0.05) were closely associated with neonatal sepsis. Sex (OR=1.107, P>0.05), polymorphisms in rs3804099(OR=0.876; P>0.05) and polymorphisms in rs5744105(OR=0.820; P>0.05) genes were not risk factors for neonatal sepsis. Conclusions TLR2 and 5 polymorphisms (rs5743708, rs3804099 and rs5744105) may not serve as the susceptible gene for sepsis in newborn infants.
关键词
败血症 /
Toll样受体 /
单核苷酸多态性 /
基因易感性 /
新生儿
Key words
Sepsis /
Toll-like receptor /
Single nucleotide polymorphism /
Genetic susceptibility /
Newborn infant
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基金
无锡市科技发展指导性计划项目(SCZ00N1105);无锡市卫生局面上项目(MS201401)。
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