Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation

CHENG Ya-Ying; LV Hong-Yan; WANG Xin; SONG Guang-Yao

doi:10.7499/j.issn.1008-8830.2014.10.019

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Chinese Journal of Contemporary Pediatrics ›› 2014, Vol. 16 ›› Issue (10) : 1051-1056. DOI: 10.7499/j.issn.1008-8830.2014.10.019

EXPERIMENTAL RESEARCH

Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation

CHENG Ya-Ying¹, LV Hong-Yan², WANG Xin¹, SONG Guang-Yao³

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Abstract

Objective To investigate the expression of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats with intrauterine growth retardation (IUGR) and its significance. Methods The IUGR animal model was established by feeding rats low-protein diets during their pregnancy. Newborn rats were divided into catch-up growth, non-catchup growth and control groups. Protein and mRNA levels of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats were determined by RT-PCR and Western blot, respectively. Results Nesfatin-1/NUCB2 mRNA and protein were expressed in the gastric mucosa of rats immediately after birth, and their expression increased in an age-dependent manner in all three groups. Furthermore, the level of nesfatin-1/NUCB2 in the catch-up growth group was higher than that in the control group before weaning, whereas there was no significant difference in nesfatin-1/NUCB2 expression between the two groups after weaning. The level of nesfatin-1/NUCB2 in the non-catch-up growth group was lower than that in the catch-up growth group during the whole observation period. The level of ghrelin in the catch-up growth group was higher than that in the control group starting from day 12 after birth, whereas there was no significant difference in ghrelin expression between the two groups after weaning. The level of ghrelin in the non-catch-up growth group was lower compared with those in the catch-up growth and control groups from days 12 to 28 after birth. Conclusions Nesfatin-1 and ghrelin are co-expressed in the gastric mucosa of rats with IUGR after birth and interact with each other to produce long-term nutritional regulation.

Key words

Intrauterine growth retardation / Nesfatin-1/NUCB2 / Ghrelin / Catch-up growth / Rats

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CHENG Ya-Ying, LV Hong-Yan, WANG Xin, SONG Guang-Yao. Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(10): 1051-1056 https://doi.org/10.7499/j.issn.1008-8830.2014.10.019

References

[1] Vieau D. Perinatal nutritional programming of health andmetabolic adult disease[J]. World J Diabetes, 2011, 2(9): 133-136.
[2] Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1as a satiety molecule in the hypothalamus[J]. Nature, 2006,443(7112): 709-712.
[3] Kojima M, Hosoda H, Date Y, et al. Ghrelin is a growthhormone-releasing acylated peptide from stomach[J]. Nature,1999, 402(6762): 656-660.
[4] Cheng YY, Zhao XM, Cai BP, et al. Nesfatin-1 in newborns:relationship with endocrine and metabolic and anthropometricmeasures[J]. J Pediatr Endocr Met, 2012, 25(7-8): 727-732.
[5] 程亚颖, 宫丽芬, 宋光耀, 等. 不同胎龄及体重新生儿血Ghrelin、胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3、胰岛素水平检测分析[J]. 中国妇幼保健杂志, 2011,26(26): 4050-4052.
[6] Mohan H, Unniappan S. Ontogenic pattern of nucleobindin-2/nesfatin-1 expression in the gastroenteropancreatic tissues andserum of Sprague Dawley rats[J]. Regul Pept, 2012, 175(1-3):61-69.
[7] Coupé B1, Amarger V, Grit I, et al. Nutritional programmingaffects hypothalamic organization and early response toleptin[J]. Endocrinology, 2010, 151(2): 702-713.
[8] Atsuchi K, Asakawa A, Ushikai M, et al. Centrally administerednesfatin-1 inhibits feeding behaviour and gastroduodenal motility in mice[J]. Neuroreport, 2010, 21(15): 1008-1011.
[9] Shimizu H, Oh-I S, Hashimoto K, et al. Peripheraladministration of nesfatin-1 reduces food intake in mice: theleptin-independent mechanism [J]. Endocrinology, 2009, 150(2):662-671.
[10] Stengel A, Goebel M, Wang L, et al. Central nesfatin-1reduces darkphase food intake and gastric emptying in rats:differential role of corticotropin-releasing factor 2 receptor[J].Endocrinology, 2009, 150(11): 4911-4919.
[11] Stengel A, Goebel M, Yakubov I, et al. Identification andcharacterization of nesfatin-1 immunoreactivity in endocrinecell types of the rat gastric oxyntic mucosa[J]. Endocrinology,2009, 150(1): 232-238.
[12] Zhang AQ, Li XL, Jiang CY, et al. Expression of nesfatin-1/NUCB2 in rodent digestive system[J]. World J Gastroenterol,2010, 16(14): 1735-1741.
[13] Kerbel B1, Unniappan S. Nesfatin-1 suppresses energy intake,co-localises ghrelin in the brain and gut, and alters ghrelin,cholecystokinin and orexin mRNA expression in goldfish[J]. JNeuroendocrinol, 2012, 24(2): 366-377.
[14] Moesgaard SG, Ahrén B, Carr RD, et al. Effects of highfatfeeding and fasting on ghrelin expression in the mousestomach[J]. Regul Pept, 2004, 120(1-3): 261-267.
[15] Aydin S, Sahin I, Ozkan Y, et al. Examination of the tissueghrelin expression of rats with diet-induced obesity usingradioimmunoassay and immunohistochemical methods[J]. MolCell Biochem, 2012, 365(1-2): 165-173.
[16] 杨慧明, 毛萌, 杨凡, 等. Grelin 及受体GHSR 表达变化与宫内发育受限仔鼠追赶生长的关系[J]. 中国当代儿科杂志,2010, 12(7): 563-568.
[17] Stengel A, Goebel M, Wang LX, et al. Ghrelin, des-acyl ghrelinand nesfatin-1 in gastric X/A-like cells: Role as regulators offood intake and body weight[J]. Peptides, 2010, 31(2): 357-369.