Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics

WANG Li-Li; ZHENG Shou-Yan; REN Luo; XIAO Qiu-Yan; LONG Xiao-Ru; LUO Jian; LI Qu-Bei; DENG Yu; XIE Xiao-Hong; LIU En-Mei

doi:10.7499/j.issn.1008-8830.2016.05.002

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (5) : 386-390. DOI: 10.7499/j.issn.1008-8830.2016.05.002

CLINICAL RESEARCH

Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics

WANG Li-Li¹, ZHENG Shou-Yan¹, REN Luo¹, XIAO Qiu-Yan¹, LONG Xiao-Ru¹, LUO Jian², LI Qu-Bei², DENG Yu², XIE Xiao-Hong², LIU En-Mei²

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Abstract

Objective To observe the levels of pulmonary surfactant proteins A and D (SP-A, SP-D) in bronchoalveolar lavage fluid (BALF) of children with pneumonia, and to explore their relationships with clinical characteristics. Methods Thirty-five children with pneumonia were enrolled in this study. Differential cell counts were obtained by Countstar counting board. The levels of SP-A and SP-D in BALF were detected using ELISA. Results In children with pneumonia, SP-D levels were significantly higher than SP-A levels (P<0.001). SP-D levels were negatively correlated with the neutrophil percentage in BALF (r_s=-0.5255, P<0.01). SP-D levels in BALF in children with increased blood C-reactive protein levels (>8 mg/L) were significantly lower than in those with a normal level of C-reactive protein (P<0.05). Compared with those in children without wheezing, SP-D levels in children with wheezing were significantly lower (P<0.01). There was no correlation between SP-A levels and clinical characteristics. Conclusions SP-D levels in BALF are significantly higher than SP-A levels, and have a certain correlation with clinical characteristics in children with pneumonia. As a protective factor, SP-D plays a more important role than SP-A in regulating the immune and inflammatory responses.

Key words

Pneumonia / Pulmonary surfactant protein / Bronchoalveolar lavage fluid / Child

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WANG Li-Li, ZHENG Shou-Yan, REN Luo, XIAO Qiu-Yan, LONG Xiao-Ru, LUO Jian, LI Qu-Bei, DENG Yu, XIE Xiao-Hong, LIU En-Mei. Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(5): 386-390 https://doi.org/10.7499/j.issn.1008-8830.2016.05.002

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