Role of serum neutrophil elastase determination in the diagnosis of acute exacerbation of asthma in preschool children

YU Zhi-Wei; HUANG Song-Ming

doi:10.7499/j.issn.1008-8830.2016.05.004

PDF(1202 KB)

Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (5) : 396-399. DOI: 10.7499/j.issn.1008-8830.2016.05.004

CLINICAL RESEARCH

Role of serum neutrophil elastase determination in the diagnosis of acute exacerbation of asthma in preschool children

YU Zhi-Wei¹, HUANG Song-Ming²

Author information +

History +

Abstract

Objective To study the role of serum neutrophil elastase (NE) level in acute exacerbation of asthma in preschool children. Methods A total of 85 preschool children who were diagnosed with asthma between January 2008 and January 2010 were classified into acute exacerbation group (n=44) and non-acute exacerbation group (n=41). Thirty-five children who received physical examination served as the control group. The enzyme-linked immunosorbent assay was used to determine the serum levels of NE and interleukin-8 (IL-8). The receiver operating characteristic (ROC) curve was used for NE evaluation. Results Both the acute and non-acute exacerbation groups had higher serum levels of NE and IL-8 than the control group, and the acute exacerbation group had significantly higher serum levels of NE and IL-8 than the non-acute exacerbation group (P<0.05). The serum level of NE was positively correlated with that of IL-8 (r=0.48, P<0.05). With serum NE level >27.73 μg/L as the cut-off value for diagnosing acute exacerbation of asthma, the sensitivity was 65.9%, the specificity was 95.1%, and the area under the ROC curve was 0.87 (P<0.01). Conclusions The determination of serum NE level in preschool children with asthma helps to diagnose the acute exacerbation of asthma.

Key words

Asthma / Neutrophil elastase / Receiver operating characteristic curve / Preschool child

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YU Zhi-Wei, HUANG Song-Ming. Role of serum neutrophil elastase determination in the diagnosis of acute exacerbation of asthma in preschool children[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(5): 396-399 https://doi.org/10.7499/j.issn.1008-8830.2016.05.004

References

[1] Nair P, Aziz-Ur-Rehman A, Radford K. Therapeutic implications of 'neutrophilic asthma'[J]. Curr Opin Pulm Med, 2015, 21(1):33-38.
[2] Mann BS, Chung KF. Blood neutrophil activation markers in severe asthma: lack of inhibition by prednisolone therapy[J]. Respir Res, 2006, 7(1): 59.
[3] Baines KJ, Simpson JL, Wood LG, et al. Systemic upregulation of neutrophil α-defensins and serine proteases in neutrophilic asthma[J]. Thorax, 2011, 66(11): 942-947.
[4] Ducharme FM, Dell SD, Radhakrishnan D, et al. Diagnosis and management of asthma in preschoolers: A Canadian Thoracic Society and Canadian Paediatric Society position paper[J]. Can Respir J, 2015, 22(3): 135-143.
[5] Wood LG, Baines KJ, Fu J, et al. The neutrophilic inflammatory phenotype is associated with systemic inflammation in asthma[J]. Chest, 2012, 142(1): 86-93.
[6] Chu YT, Chiang W, Wang TN, et al. Changes in serum eotaxin and eosinophil cationic protein levels, and eosinophil count during treatment of childhood asthma[J]. J Microbiol Immunol Infect, 2007, 40(2): 162-167.
[7] 安淑华, 田文秋, 李金英. 呼出气一氧化氮检测在幼儿支气管哮喘中的应用分析[J]. 中国当代儿科杂志, 2015, 17(2): 134-137.
[8] Yoshihara S, Yamada Y, Abe T, et al. Association of epithelial damage and signs of neutrophil mobilization in the airways during acute exacerbations of paediatric asthma[J]. Clin Exp Immunol, 2006, 144(2): 212-216.
[9] Lee TH, Song HJ, Park CS. Role of inflammasome activation in development and exacerbation of asthma[J]. Asia Pac Allergy, 2014, 4(4): 187-196.
[10] Hosoki K, Ying S, Corrigan C, et al. Analysis of a Panel of 48 Cytokines in BAL Fluids Specifically Identifies IL-8 Levels as the Only Cytokine that Distinguishes Controlled Asthma from Uncontrolled Asthma, and Correlates Inversely with FEV1[J]. PLoS One, 2015, 10(5): e0126035.
[11] Matsuoka H, Niimi A, Matsumoto H, et al. Inflammatory subtypes in cough-variant asthma: association with maintenance doses of inhaled corticosteroids[J]. Chest, 2010, 138(6): 1418-1425.
[12] Simpson JL, Powell H, Boyle MJ, et al. Clarithromycin targets neutrophilic airway inflammation in refractory asthma[J]. Am J Respir Crit Care Med, 2008, 177(2): 148-155.
[13] Brusselle GG, Vanderstichele C, Jordens P, et al. Azithromycin for prevention of exacerbations in severe asthma (AZISAST): a multicentre randomised double-blind placebo-controlled trial[J]. Thorax, 2013, 68(4): 322-329.
[14] Tsai YF, Hwang TL. Neutrophil elastase inhibitors: a patent review and potential applications for inflammatory lung diseases (2010-2014)[J]. Expert Opin Ther Pat, 2015, 25(10): 1145-1158.