Clinical value of fluorescence lateral flow immunoassay in diagnosis of influenza A in children

GUO Chun; ZHONG Li-Li; YI Hong-Ling; CHEN Min

doi:10.7499/j.issn.1008-8830.2016.12.015

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (12) : 1272-1276. DOI: 10.7499/j.issn.1008-8830.2016.12.015

CLINICAL RESEARCH

Clinical value of fluorescence lateral flow immunoassay in diagnosis of influenza A in children

GUO Chun, ZHONG Li-Li, YI Hong-Ling, CHEN Min

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Abstract

Objective To evaluate the clinical value of a new type of fluorescence lateral flow immunoassay in rapid detection of influenza A virus. Methods A total of 378 samples of nasopharyngeal secretions were collected from 378 children with influenza-like symptoms to detect the influenza A virus by fluorescence lateral flow immunoassay, colloidal gold immunoassay, and RT-PCR between July 2015 and August 2015. Results Of the 378 samples, 81 (21.4%) were positive for influenza A virus by RT-PCR. Compared with RT-PCR, the sensitivities of fluorescence lateral flow immunoassay and colloidal gold immunoassay were 90.1% (73/81) and 75.3% (61/81), respectively, and the specificities were 99.3% (295/297) and 98.3% (292/297), respectively. The average threshold cycle (Ct) value for the positive samples detected by the fluorescence lateral flow immunoassay (30.6) was higher than that for the positive samples detected by the colloidal gold immunoassay (28.7). Conclusions Compared with colloidal gold immunoassay, fluorescence lateral flow immunoassay has higher sensitivity, specificity, and concordance rate with RT-PCR, suggesting that it can be used for early screening and diagnosis of influenza A.

Key words

Influenza A / Rapid diagnosis / Fluorescence lateral flow immunoassay / Child

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GUO Chun, ZHONG Li-Li, YI Hong-Ling, CHEN Min. Clinical value of fluorescence lateral flow immunoassay in diagnosis of influenza A in children[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(12): 1272-1276 https://doi.org/10.7499/j.issn.1008-8830.2016.12.015

References

[1] Liaw YF, Chu CM. Hepatitis B virus infection[J]. Lancet, 2009, 373(9663):582-592.
[2] Komatsu H, Inui A. Hepatitis B virus infection in children[J]. Expert Rev Anti Infect Ther, 2015, 13(4):427-450.
[3] Zeng Z. Human genes involved in hepatitis B virus infection[J]. World J Gastroenterol, 2014, 20(24):7696-7706.
[4] 周琴, 郜玉峰, 赵小苗, 等. IL-4基因多态性与HBV遗传易感性的研究[J]. 中华疾病控制杂志, 2014, 18(10):968-971.
[5] Motavaf M, Safari S, Alavian SM. Interleukin 18 gene promoter polymorphisms and susceptibility to chronic hepatitis B infection:a review study[J]. Hepat Mon, 2014, 14(7):e19879.
[6] Wu L, Chen L, Zhou L, et al. Association of interleukin 18 gene promoter polymorphisms with HBV recurrence after liver transplantation in Han Chinese population[J]. Hepat Mon, 2011, 11(6):469-474.
[7] 费筠, 陈月, 吴文苑. 白细胞介素与HBV感染的关系[J]. 临床肝胆病杂志, 2014, 30(2):174-177.
[8] 夏旭, 杨钦河. 白细胞介素-19的研究近况[J]. 国外医学(免疫学分册), 2002, 25(6):324-328.
[9] Liao YC, Liang WG, Chen FW, et al. IL-19 induces production of IL-6 and TNF-alpha and results in cell apoptosis through TNF-alpha[J]. J Immunol, 2002, 169(8):4288-4297.
[10] 中华医学会肝病学分会, 中华医学会感染病学分会. 慢性乙型肝炎防治指南[J]. 中华传染病杂志, 2005, 23(6):421-431.
[11] Zhang PA, Wu JM, Li Y, et al. Association of polymorphisms of interleukin-18 gene promoter region with chronic hepatitis B in Chinese Han population[J]. World J Gastroenterol, 2005, 11(11):1594-1598.
[12] Miyazoe S, Hamasaki K, Nakata K, et al. Influence of interleukin-10 gene promoter polymorphisms on disease progression in patients chronically infected with hepatitis B virus[J]. Am J Gastroenterol, 2002, 97(8):2086-2092.
[13] Shin HD, Park BL, Kim LH, et al. Interleukin 10 haplotype associated with increased risk of hepatocellular carcinoma[J]. Hum Mol Genet, 2003, 12(8):901-906.
[14] Zhu QR, Ge YL, Gu SQ, et al. Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection[J]. Chin Med J (Engl), 2005, 118(19):1604-1609.
[15] Moudi B, Heidari Z, Mahmoudzadeh-Sagheb H, et al. Association between IL-10 gene promoter polymorphisms (-592 A/C,-819 T/C,-1082 A/G) and susceptibility to HBV infection in an Iranian population[J]. Hepat Mon, 2016, 16(2):e32427.
[16] Truelove AL, Oleksyk TK, Shrestha S, et al. Evaluation of IL10, IL19 and IL20 gene polymorphisms and chronic hepatitis B infection outcome[J]. Int J Immunogenet, 2008, 35(3):255-264.
[17] Ryckman KK, Fielding K, Hill AV, et al. Host genetic factors and vaccine-induced immunity to HBV infection:haplotype analysis[J]. PLoS One, 2010, 5(8):e12273.
[18] Yamamoto-Furusho JK, Álvarez-León E, Fragoso JM, et al. Protective role of interleukin-19 gene polymorphisms in patients with ulcerative colitis[J]. Hum Immunol, 2011, 72(11):1029-1032.
[19] Shao ZJ, Zhang L, Xu JQ, et al. Mother-to-infant transmission of hepatitis B virus:a Chinese experience[J]. J Med Virol, 2011, 83(5):791-795.
[20] Yucesoy B, Talzhanov Y, Johnson VJ, et al. Genetic variants within the MHC region are associated with immune responsiveness to childhood vaccinations[J]. Vaccine, 2013, 31(46):5381-5391.