Abstract：Objective This study aims to investigate the interleukin 13 (IL 13) expression in the human mesangial cells (HMC) and its effect on expressions of cytokines synthesized by HMC so as to study the role of IL 13 in the inflammatory process of glomerulonephritis. Methods The HMC were cultured and treated with LPS and/or recombinant human IL 13. The IL 13 mRNA expression and the IL 13 protein level in the cultured HMC were detected by semiquantitative reverse transcription polymerase chain reaction (RT PCR) and enzyme linked immunosorbent assay (ELISA) respectively. The effects of IL 13 on the expressions of proinflammatory cytokines, chemokines and profibrogenic cytokine in the HMC were determined by ribonuclease protection assay (RPA). The cultured HMC without LPS or recombinant human IL 13 stimulation were used as the controls. Results The IL 13 mRNA expression and IL 13 protein were undetected in the controls without stimulation. The IL 13 mRNA expression in the HMC with LPS stimulation was induced as early as 12 hrs after LPS stimulation, reached a peak at 48 hrs and remained high level until 72 hrs. The activation of HMC with LPS resulted in the induction of IL 13 mRNA expression in a dose and time dependent way. The IL 13 protein was induced 24 hrs after LPS stimulation and was further increased with stimulation time. The recombinant IL 13 inhibited TNF α, IL 1α, IL 1β, MCP 1, IL 8 and TGF β1 mRNA expressions were induced by LPS in a dose dependent way. The expressions of TNF α, IL 1α, IL 1β, MCP 1, IL 8, and TGF β1 mRNA were increased after endogenously produced IL 13 was neutralized with anti IL 13 mAb. Conclusions LPS can induce the IL 13 expression in HMC. The mesangial cell derived IL 13 can inhibit the production of proinflammatory cytokines, chemokines, and profibrogenic cytokine synthesized by HMC.
ZHANG Ai-Hua,DING Gui-Xia,WU Yuan-Jun et al. Effect of mesangial cell2derived interleukin213 on expression of cytokines synthesized human mesangial cells[J]. CJCP, 2004, 6(2): 85-88.
