OBJECTIVE:To study the changes of blood nitric oxide (NO), lipid peroxide (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and glutathione (GSH) levels and the effect of impulsive therapy of methylprednisolone (MP) and cyclophosphamide (CTX) on children with lupus nephritis (LN). METHODS: The blood levels of NO, LPO, SOD, GSH-px and GSH were detected by chemical analysis in 18 active patients with this disorder (the active group) before and after MP and CTX impulsive therapy. Of the 18 cases, 16 who had become to inactive after treatment were recruited in this study. Twenty three healthy children were served as the control group. RESULTS: Except the decreased GSH level, the levels of NO, LPO, SOD and GSH-px in the inactive group did not differ from those of the control group. The levels of NO and LPO in the active group were significantly higher, SOD, GSH px and GSH were significantly lower than those of the control group and the inactive group (P< 0.05 or 0.01 ). NO and LPO in the active group were positively correlated with anti-dsDNA antibody, 24-hour proteinuria, blood urea nitrogen, serum creatinine and erythrocyte sedimentation rate, negatively correlated with complement C 3, complement C 4 and endogenous creatinine clearance rate. SOD, GSH-px and GSH were negatively correlated with anti-dsDNA antibody, 24-hour proteinuria, blood urea nitrogen, serum creatinine and erythrocyte sedimentation rate, positively correlated with compliment C 3, compliment C 4 and endogenous creatinine clearance rate. The levels of the five parameters (NO, LOP, SOD, GSH px and GSH) in children with LN complicated by renal damage were significantly different from those in the control group and those patients without renal damage. CONCLUSIONS: The levels of NO and LPO would decrease and the levels of SOD, GSH-px GSH would elevate after treatment with MP and CTX. NO, LPO, SOD, GSH-px and GSH may take part in the pathogenesis of LN. Detecting the above five parameters may help in monitoring the progress, the extent of renal damage of LN and therapeutic effect for LN patients."/>
甲基强的松龙与环磷酰胺冲击治疗对狼疮肾炎患儿抗氧化功能的影响
Abstract：OBJECTIVE:To study the changes of blood nitric oxide (NO), lipid peroxide (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and glutathione (GSH) levels and the effect of impulsive therapy of methylprednisolone (MP) and cyclophosphamide (CTX) on children with lupus nephritis (LN). METHODS: The blood levels of NO, LPO, SOD, GSH-px and GSH were detected by chemical analysis in 18 active patients with this disorder (the active group) before and after MP and CTX impulsive therapy. Of the 18 cases, 16 who had become to inactive after treatment were recruited in this study. Twenty three healthy children were served as the control group. RESULTS: Except the decreased GSH level, the levels of NO, LPO, SOD and GSH-px in the inactive group did not differ from those of the control group. The levels of NO and LPO in the active group were significantly higher, SOD, GSH px and GSH were significantly lower than those of the control group and the inactive group (P< 0.05 or 0.01 ). NO and LPO in the active group were positively correlated with anti-dsDNA antibody, 24-hour proteinuria, blood urea nitrogen, serum creatinine and erythrocyte sedimentation rate, negatively correlated with complement C 3, complement C 4 and endogenous creatinine clearance rate. SOD, GSH-px and GSH were negatively correlated with anti-dsDNA antibody, 24-hour proteinuria, blood urea nitrogen, serum creatinine and erythrocyte sedimentation rate, positively correlated with compliment C 3, compliment C 4 and endogenous creatinine clearance rate. The levels of the five parameters (NO, LOP, SOD, GSH px and GSH) in children with LN complicated by renal damage were significantly different from those in the control group and those patients without renal damage. CONCLUSIONS: The levels of NO and LPO would decrease and the levels of SOD, GSH-px GSH would elevate after treatment with MP and CTX. NO, LPO, SOD, GSH-px and GSH may take part in the pathogenesis of LN. Detecting the above five parameters may help in monitoring the progress, the extent of renal damage of LN and therapeutic effect for LN patients.
JIANG Hong,LUO Gang,WANG Shu-Lan. Effect of Methylprednisolone and Cyclophosphamide Impulsive Therapy on the Anti-Oxidation Function in Children with Lupus Nephritis[J]. CJCP, 2003, 5(3): 229-233.
