Abstract：Objective To study the excitatory amino acid (EAA) neurotransmitter concentration in bilirubin induced encephalopathy. Methods Guinea pigs with bilirubin-induced encephalopathy were fixed in the stereotaxic frame. The microdialysis probe was inserted in the hippocampus zone, Kreb's ringer was irrigated and extracellular liquid was collected for analyses with high performance liquid chromatography (HPLC). Results The concentration of EAA neurotransmitter Gly in the extracellular liquid of the bilirubin precipitated brain was 21.35±4.87 μM,significantly higher than that in the control group (14.02±1.56μM). EAA neurotransmitter Glu and Asp did not show any significant change in the animal model compared with the control. Conclusions The increase of EAA neurotransmitter Gly concentration in the extracellular liquid of the bilirubin precipitated brain tissues might increase the sensitivity of the ligand Asp and Glu to the NMDA Receptor.
BEN Xiao-Ming,CHEN Shun-Nian,QIN Yu-Ming et al. Microdialysis of Hippocampus for Measurement of the Neurotransmitter in Bilirubin Induced Encephalopathy Guinea Pigs in Vivo[J]. CJCP, 2000, 2(1): 18-20.
