维生素 D 缺乏对子代大鼠肺形态结构及血小板源性生长因子-A 表达的影响

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摘要目的：研究孕期维生素 D（VitD）缺乏对子代大鼠肺形态发育及血小板源性生长因子-A（PDGF-A）表达的影响。方法：雌性Sprague-Dawley (SD) 大鼠随机分为对照组、VitD 缺乏模型组（每组6只）。对照组正常饲养；模型组予以避光、不含 VitD 的饲料喂养，2周后与成熟 SD 雄性大鼠交配，每组取孕20 d的胎肺及生后1 d 新生鼠肺，光镜及电镜下观察肺形态结构， RT-PCR 及 Western blot 分别检测肺组织PDGF-A mRNA及蛋白水平的表达。结果：光镜下，模型组子鼠肺泡平均表面积、平均呼吸膜周径均小于对照组（P<0.05），平均肺泡间隔厚度大于对照组（P<0.05）；电镜下，模型组子鼠的板层小体数量明显少于对照组，成熟细胞器较少见。RT-PCR 及 Western blot结果显示模型组子鼠肺组织PDGF-A mRNA和蛋白表达水平均低于对照组（P<0.05）。结论：孕期 VitD 缺乏抑制孕晚期胎鼠及新生大鼠的肺形态发育。VitD 缺乏能显著抑制肺组织 PDGF-A 表达；PDGF-A表达减低可能是VitD缺乏抑制大鼠肺发育的重要机制之一。

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	顾海燕
	赵德育
	王全

关键词 ：维生素D, 肺形态, 血小板源性生长因子-A, 大鼠

Abstract：OBJECTIVE: To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A (PDGF-A) expression in rat offspring. METHODS: Sprague-Dawley (SD) female rats were randomly divided into two groups: normal control and vitamin D deficiency, with 6 rats in each group. The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D. After 2 weeks, the rats were mated with normal SD male rats. The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Western blot method respectively. RESULTS: Under the light microscope, smaller alveolar space, smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compared with the controls (P<0.05). Under the electronic microscope, fewer lamellar bodies but more glycogen deposition in intracytoplasm were observed in the lungs of fetal rats from the vitamin D deficiency group compared with the controls. There was an increased number of empty lamellar bodies in neonatal rats from the vitamin D deficiency group. The levels of PDGF-A mRNA and protein in lungs of fetal and neonatal rats from the vitamin D deficiency group were significantly lower than the controls (P<0.05). CONCLUSIONS: Maternal vitamin D deficiency during pregnancy may inhibit the development of lung morphogenesis and PDGF-A expression in late fetal and neonatal rats. The low expression of PDGF-A may be involved in the inhibitory effect of vitamin D deficiency on the lung development.

Key words： Vitamin D Lung morphogenesis Platelet-derived growth factor-A Rats

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R-33

引用本文:

顾海燕,赵德育,王全. 维生素 D 缺乏对子代大鼠肺形态结构及血小板源性生长因子-A 表达的影响[J]. 中国当代儿科杂志, 2011, 13(4): 326-330.

GU Hai-Yan,ZHAO De-Yu,WANG Quan. Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring[J]. CJCP, 2011, 13(4): 326-330.

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http://www.zgddek.com/CN/ 或 http://www.zgddek.com/CN/Y2011/V13/I4/326

	［1］Marin L, Dufour ME, Nguyen TM, Tordet C, Garabedian M. Maturational changes induced by 1 alpha, 25-dihydroxyvitamin D3 in type II cells from fetal rat lung explants［J］. Am J Physiol, 1993, 265(1 Pt 1): L45-L52.
	［2］Rehan VK, Torday JS, Peleg S, Gennaro L, Vouros P, Padbury J, et al. 1 alpha, 25-dihydroxy-3-epi-vitamin D3, a natural metabolite of 1 alpha, 25-dihydroxy vitamin D3: production and biological activity studies in pulmonary alveolar type II cells［J］. Mol Genet Metab, 2002, 76 (1): 46-56.
	［3］Nguyen TM, Guillozo H, Marin L, Tordet C, Koite S, Garabedian M. Evidence for a Vitamin D paracrine system regulating maturation of developing rat lung epithelium［J］. Am J Physiol, 1996, 271(15): L392-L399.
	［4］王华，黎海芪，杨锡强. 钙、维生素D 缺乏大鼠长骨成骨细胞的维生素D 受体水平［J］. 中华内分泌代谢杂志，2003，19(2): 146-147.
	［5］Gaultier C, Harf A, Balmain N, Cuisinier-Gleizes P, Mathieu H. Lung mechanics in rachitic rats［J］. Am Rev Respir Dis, 1984, 130(6): 1108-1110.
	［6］Andiran N, Yordam N, Oz-n A. Risk factors for vitamin D deficiency in breast-fed newborns and their mothers［J］. Nutrition, 2002, 18(1): 47-50.
	［7］Siddiqui TS. Respiratory distress: a rare presentation of rickets［J］. J Ayub Med Coll Abbottabad, 2006, 18(3): 67-68.
	［8］Samtani MN, Pyszczynski NA, Dubois DC, Almon RR, Jusko WJ. Modeling glucocorticoid mediated fetal lung maturation: I. Temporal patterns of corticosteroids in rat pregnancy［J］. J Pharmacol Exp Ther, 2006, 317(1): 117-126.
	［9］Nguyen M, Trubert CL, Rizk-Rabin M, Rehan VK, Besancon F, Cayre YE, et al. 1, 25-Dihydroxyvitamin D3 and fetal lung maturation: immunogold detection of VDR expression in pneumocytes type II cells and effect on fructose 1, 6 bisphosphatase［J］. J Steroid Biochem Mol Biol, 2004, 89-90(1-5):93-97.
	［10］阚清，顾筱琪，周小玉. 正常胎儿肺发育调节研究进展［J］.实用儿科临床杂志，2006，21(20): 1428-1429.
	［11］Li J, Hoyle GW. Overexpression of PDGF-A in the lung epithelium of transgenic mice produces a lethal phenotype associated with hyperplasia of mesenchymal cells［J］. Dev Biol, 2001, 239 (2): 338-349.
	［12］Assaad AM, Kawut SM, Arcasoy SM, Rosenzweig EB, Wilt JS, Sonett JR, et al. Platelet-derived growth factor is increased in pulmonary capillary hemangiomatosis［J］. Chest, 2007, 131(3): 850-855.
	［13］Pedigo N, Zhang H, Koszewski NJ, Kaetzel DM. A 5′-distal element mediates vitamin D-inducibility of PDGF-A gene transcription［J］. Growth Factors, 2003, 21(3-4):151-160.
	［14］Pedigo NG, Zhang H, Mishra A, McCorkle JR, Ormerod AK, Kaetzel DM. Retinoic acid inducibility of the human PDGF-a gene is mediated by 5′-distal DNA motifs that overlap with basal enhancer and vitamin D response elements［J］. Gene Expr, 2007, 14(1): 1-12.