Alterations in the intestinal microbiota of preterm infants with neurodevelopmental impairments: a prospective cohort study
LI Yan, LU Si-Liang, MO Yan, JING Lian-Fang, YAO Li-Ping, TAN Wei, WEI Qiu-Fen
Neonatal Medical Center, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region/Guangxi Clinical Research Center for Pediatric Diseases, Nanning 530003, China
Abstract：Objective To investigate the difference in intestinal microbiota between preterm infants with neurodevelopmental impairment (NDI) and those without NDI. Methods In this prospective cohort study, the preterm infants who were admitted to the neonatal intensive care unit of Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region from September 1, 2019 to September 30, 2021 were enrolled as subjects. According to the assessment results of Gesell Developmental Scale at the corrected gestational age of 1.5-2 years, they were divided into two groups: normal (n=115) and NDI (n=100). Fecal samples were collected one day before discharge, one day before introducing solid food, and at the corrected gestational age of 1 year. High-throughput sequencing was used to compare the composition of intestinal microbiota between groups. Results Compared with the normal group, the NDI group had a significantly higher Shannon diversity index at the corrected gestational age of 1 year (P<0.05). The principal coordinate analysis showed a significant difference in the composition of intestinal microbiota between the two groups one day before introducing solid food and at the corrected gestational age of 1 year (P<0.05). Compared with the normal group, the NDI group had a significantly higher abundance of Bifidobacterium in the intestine at all three time points, a significantly higher abundance of Enterococcus one day before introducing solid food and at the corrected gestational age of 1 year, and a significantly lower abundance of Akkermansia one day before introducing solid food (P<0.05). Conclusions There are significant differences in the composition of intestinal microbiota between preterm infants with NDI and those without NDI. This study enriches the data on the characteristics of intestinal microbiota in preterm infants with NDI and provides reference for the microbiota therapy and intervention for NDI in preterm infants.
LI Yan,LU Si-Liang,MO Yan et al. Alterations in the intestinal microbiota of preterm infants with neurodevelopmental impairments: a prospective cohort study[J]. CJCP, 2023, 25(7): 689-696.
Young S, González RA, Mullens H, et al. Neurodevelopmental disorders in prison inmates: comorbidity and combined associations with psychiatric symptoms and behavioural disturbance[J]. Psychiatry Res, 2018, 261: 109-115. PMID: 29291476. DOI: 10.1016/j.psychres.2017.12.036.
GuangXi Cooperative Research Group for Extremely Preterm Infants, Li Y, Meng DH, et al. Neurodevelopmental outcomes of extremely preterm infants in southern China: a multicenter study[J]. Early Hum Dev, 2019, 133: 5-10. PMID: 30991237. DOI: 10.1016/j.earlhumdev.2019.04.002.
Parsons E, Claud K, Petrof EO. The infant microbiome and implications for central nervous system development[J]. Prog Mol Biol Transl Sci, 2020, 171: 1-13. PMID: 32475519. DOI: 10.1016/bs.pmbts.2020.04.007.
Schwarz E, Maukonen J, Hyyti?inen T, et al. Analysis of microbiota in first episode psychosis identifies preliminary associations with symptom severity and treatment response[J]. Schizophr Res, 2018, 192: 398-403. PMID: 28442250. DOI: 10.1016/j.schres.2017.04.017.
Matamoros S, Gras-Leguen C, Le Vacon F, et al. Development of intestinal microbiota in infants and its impact on health[J]. Trends Microbiol, 2013, 21(4): 167-173. PMID: 23332725. DOI: 10.1016/j.tim.2012.12.001.
Beghetti I, Barone M, Turroni S, et al. Early-life gut microbiota and neurodevelopment in preterm infants: any role for Bifidobacterium?[J]. Eur J Pediatr, 2022, 181(4): 1773-1777. PMID: 34842970. DOI: 10.1007/s00431-021-04327-1.
Cani PD, Depommier C, Derrien M, et al. Akkermansia muciniphila: paradigm for next-generation beneficial microorganisms[J]. Nat Rev Gastroenterol Hepatol, 2022, 19(10): 625-637. PMID: 35641786. DOI: 10.1038/s41575-022-00631-9.
