ORMDL3 polymorphisms and their relationship with OPN and TGF-β1 levels in children with asthma in Hunan, China: an analysis of 98 cases

YANG Ai-Mei; HUANG Rong; JIN Shi-Jie

doi:10.7499/j.issn.1008-8830.2016.04.009

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (4) : 324-328. DOI: 10.7499/j.issn.1008-8830.2016.04.009

CLINICAL RESEARCH

ORMDL3 polymorphisms and their relationship with OPN and TGF-β1 levels in children with asthma in Hunan, China: an analysis of 98 cases

YANG Ai-Mei¹, HUANG Rong¹, JIN Shi-Jie²

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Abstract

Objective To investigate ORMDL3 polymorphisms in children with asthma in Hunan, China, and to determine the relationship between ORMDL3 polymorphisms and serum osteopontin (OPN) and transforming growth factor-β1 (TGF-β1) levels. Methods Peripheral blood samples were collected in children with asthma (n=98; astma group) or without asthma (n=30; control group) from Hunan, China. The asthma group was subdivided into atopic (n=62) and non-atopic (n=36) subgroups. Single nucleotide polymorphism (SNP) analysis was performed, and serum OPN and TGF-β1 levels were measured. Results There were no significant differences in genotype and allele frequencies of rs7216389 of the ORMDL3 gene between the asthma and control groups. The serum level of OPN in the asthma group was significantly higher than in the control group (P<0.05). Both the atopic and non-atopic subgroups showed increased serum levels of OPN compared with the control group (P<0.05). The serum level of TGF-β1 in the atopic subgroup was significantly higher than in the control group (P<0.05). The serum levels of OPN and TGF-β1 showed no significant differences in asthmatic children with different genotypes. The serum levels of OPN and TGF-β1 were in a positive linear correlation in the asthma group (r=0.620; P<0.01) and its two subgroups (r=0.734, 0.649 respectively; P<0.01). Conclusions In children from Hunan, China, the SNP (rs7216389) of ORMDL3 is not related to asthma susceptibility. OPN and TGF-β1 may be involved in the development of asthma, and they are in a positive linear correlation. The SNP (rs7216389) of ORMDL3 does not influence the expression of OPN and TGF-β1, suggesting that it may not be associated with airway remodeling.

Key words

Asthma / ORMDL3 gene / Atopic status / Single nucleotide polymorphism / Osteopontin / Transforming growth factor-β1 / Child

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YANG Ai-Mei, HUANG Rong, JIN Shi-Jie. ORMDL3 polymorphisms and their relationship with OPN and TGF-β1 levels in children with asthma in Hunan, China: an analysis of 98 cases[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(4): 324-328 https://doi.org/10.7499/j.issn.1008-8830.2016.04.009

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