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Effect of high-fat diet on expression of transient receptor potential vanilloid 1 in respiratory tract and dorsal root ganglion of mice
ZHU Lian, XU Zhi-Liang
Chinese Journal of Contemporary Pediatrics ›› 2017, Vol. 19 ›› Issue (7) : 826-831.
PDF(1419 KB)
PDF(1419 KB)
Effect of high-fat diet on expression of transient receptor potential vanilloid 1 in respiratory tract and dorsal root ganglion of mice
Objective To investigate the effect of high-fat diet on the expression of transient receptor potential vanilloid 1 (TRPV1) in the respiratory system and the dorsal root ganglion (DRG) of mice, as well as its effect on the excitability of sensory neurons. Methods A total of 20 C57BL/6 mice were randomly divided into normal-diet (ND) group and high-fat diet (HFD) group, with 10 mice in each group. The mice were given corresponding diets and body weights were monitored. After 7 weeks of feeding, lung tissue, bronchial tissue, and DRG at thoracic segments 3-4 were collected and immunohistochemical staining was performed. A patch clamp was used to measure the number of action potentials and TRPV1 current intensity in the DRG. Results After 7 weeks of feeding, the HFD group had signifcantly greater mean weight gain than the ND group (6.4±2.6 g vs 2.3±0.5 g; P < 0.001). The HFD group had signifcantly higher expression of TRPV1 in the bronchus, pulmonary alveoli, and DRG than the ND group (P < 0.05). Compared with the ND group, the HFD group had signifcant increases in the TRPV1 current intensity and number of action potentials in the DRG (P < 0.05). Conclusions High-fat diet induces a signifcant increase in body weight and leads to high expression of TRPV1 and high excitability in the respiratory system and the peripheral sensory neurons. This suggests that TRPV1 may be an important factor in the physiopathological mechanisms of bronchial hyperresponsiveness.
High-fat diet / Airway / Dorsal root ganglion / Transient receptor potential vanilloid 1 / Neural excitability / Mice
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