Molecular epidemiological characteristics of the virus in 96 children with acute diarrhea in Changdu of Tibet, China

MAO Jun-Wen, YANG Ya-Li, SHI Chang-Chun, CHEN Zhu, LI Chun, WANG Yong-Ming, LI Lin-Bin, CHEN Jun-Hua

Chinese Journal of Contemporary Pediatrics ›› 2022, Vol. 24 ›› Issue (3) : 266-272.

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Chinese Journal of Contemporary Pediatrics ›› 2022, Vol. 24 ›› Issue (3) : 266-272. DOI: 10.7499/j.issn.1008-8830.2110012
CLINICAL RESEARCH

Molecular epidemiological characteristics of the virus in 96 children with acute diarrhea in Changdu of Tibet, China

  • MAO Jun-Wen, YANG Ya-Li, SHI Chang-Chun, CHEN Zhu, LI Chun, WANG Yong-Ming, LI Lin-Bin, CHEN Jun-Hua
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Abstract

Objective To study the molecular epidemiological characteristics of the virus in children with acute viral diarrhea in Changdu of Tibet, China. Methods Fecal specimens were collected from 96 children with acute diarrhea who visited the People's Hospital of Changdu, Tibet, from November 2018 to November 2020 and were tested for adenovirus, norovirus, astrovirus, sapovirus, and rotavirus. Gene sequencing was performed for the genotypes of these viruses. Results The overall positive rate of the five viruses was 39% (37/96), among which astrovirus had the highest positive rate of 17%, followed by norovirus (9%), rotavirus (8%), adenovirus (7%), and sapovirus (5%). There was no significant difference in the positive rate of the five viruses among different age groups (P>0.05). Only the positive rate of astrovirus was significantly different among the four seasons (P<0.05). For adenovirus, 6 children had F41 type and 1 had C2 type; for norovirus, 6 had GⅠ.3 type, 1 had GⅠ.7 type, 1 had GⅡ.3 type, and 2 had GⅡ.4 Sydney_2012 type; HAstrV-1 type was observed in all children with astrovirus infection; for sapovirus, 1 child each had sporadic GⅠ.2, GⅠ.6, and GⅡ.1 sapovirus and 2 children had unknown type; 6 children had rotavirus G9[P8]. Conclusions Astrovirus and norovirus are important pathogens in children with acute diarrhea in Changdu, Tibet. The positive rate of adenovirus, norovirus, astrovirus, sapovirus, and rotavirus is not associated with age, and only the positive rate of astrovirus has obvious seasonality. F41 type is the dominant genotype of adenovirus; GⅠ.3 is the dominant genotype of norovirus; HAstrV-1 is the dominant genotype of astrovirus; sporadic GⅠ.2, GⅠ.6, and GⅡ.1 are the dominant genotypes of sapovirus; G9[P8] is the dominant genotype of rotavirus.

Key words

Viral diarrhea / Molecular epidemiology / Genotype / Child

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MAO Jun-Wen, YANG Ya-Li, SHI Chang-Chun, CHEN Zhu, LI Chun, WANG Yong-Ming, LI Lin-Bin, CHEN Jun-Hua. Molecular epidemiological characteristics of the virus in 96 children with acute diarrhea in Changdu of Tibet, China[J]. Chinese Journal of Contemporary Pediatrics. 2022, 24(3): 266-272 https://doi.org/10.7499/j.issn.1008-8830.2110012

References

1 Walker CLF, Rudan I, Liu L, et al. Global burden of childhood pneumonia and diarrhoea[J]. Lancet, 2013, 381(9875): 1405-1416. PMID: 23582727. PMCID: PMC7159282. DOI: 10.1016/S0140-6736(13)60222-6.
2 Yu JX, Jing HQ, Lai SJ, et al. Etiology of diarrhea among children under the age five in China: results from a five-year surveillance[J]. J Infect, 2015, 71(1): 19-27. PMID: 25753104. PMCID: PMC4667737. DOI: 10.1016/j.jinf.2015.03.001.
3 耿启彬, 赖圣杰, 余建兴, 等. 中国26省(直辖市、自治区)2011-2014年5岁以下儿童腹泻病例轮状病毒流行特征分析[J]. 疾病监测, 2016, 31(6): 463-470. DOI: 10.3784/j.issn.1003-9961.2016.06.006.
4 景怀琦, 黄留玉. 腹泻症候群病原学监测与检测技术[M]. 广州:中山大学出版社, 2016: 155-182.
5 Chen J, Wan CM, Gong ST, et al. Chinese clinical practice guidelines for acute infectious diarrhea in children[J]. World J Pediatr, 2018, 14(5): 429-436. PMID: 30269306. DOI: 10.1007/s12519-018-0190-2.
6 中华医学会儿科学分会消化学组, 《中华儿科杂志》编辑委员会. 中国儿童急性感染性腹泻病临床实践指南[J]. 中华儿科杂志, 2016, 54(7): 483-488. PMID: 27412736. DOI: 10.3760/cma.j.issn.0578-1310.2016.07.002.
7 Mans J. Norovirus infections and disease in lower-middle- and low-income countries, 1997-2018[J]. Viruses, 2019, 11(4): 341. PMID: 30974898. PMCID: PMC6521228. DOI: 10.3390/v11040341.
8 余建兴, 赖圣杰, 王鑫, 等. 中国27省(市、自治区)2009-2013年门诊腹泻病例诺如病毒流行特征分析[J]. 中华流行病学杂志, 2015, 36(3): 199-204. PMID: 25975393. DOI: 10.3760/cma.j.issn.0254-6450.2015.03.003.
9 商晓春, 帅慧群, 赵雪琴, 等. 2012-2019年浙江省杭州市哨点医院5岁以下儿童病毒性腹泻病原学和流行病学特征分析[J]. 疾病监测, 2020, 35(9): 809-814. DOI: 10.3784/j.issn.1003-9961.2020.09.009.
10 金晶, 张业成, 刘映乐, 等. 2015年湖北省武汉市5岁以下儿童病毒性腹泻病原学研究[J]. 疾病监测, 2018, 33(11): 940-944. DOI: 10.3784/j.issn.1003-9961.2018.11.014.
11 李静, 张婷, 邹文菁, 等. 2018-2019年湖北省5岁及以下腹泻儿童诺如病毒感染特征和病原学分析[J]. 疾病监测, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006.
12 Wang P, Goggins WB, Chan EYY. A time-series study of the association of rainfall, relative humidity and ambient temperature with hospitalizations for rotavirus and norovirus infection among children in Hong Kong[J]. Sci Total Environ, 2018, 643: 414-422. PMID: 29940452. DOI: 10.1016/j.scitotenv.2018.06.189.
13 Lopman B, Armstrong B, Atchison C, et al. Host, weather and virological factors drive norovirus epidemiology: time-series analysis of laboratory surveillance data in England and Wales[J]. PLoS One, 2009, 4(8): e6671. PMID: 19701458. PMCID: PMC2726937. DOI: 10.1371/journal.pone.0006671.
14 Glass RI, Parashar UD, Estes MK. Norovirus gastroenteritis[J]. N Engl J Med, 2009, 361(18): 1776-1785. PMID: 19864676. PMCID: PMC3880795. DOI: 10.1056/NEJMra0804575.
15 Chen SY, Chiu CH. Worldwide molecular epidemiology of norovirus infection[J]. Paediatr Int Child Health, 2012, 32(3): 128-131. PMID: 22824658. DOI: 10.1179/2046905512Y.0000000031.
16 方玉莲, 王维, 王萍, 等. 天津地区急性胃肠炎患儿诺如病毒分子流行病学分析[J]. 中国当代儿科杂志, 2019, 21(2): 139-143. PMID: 30782275. PMCID: PMC7389835. DOI: 10.7499/j.issn.1008-8830.2019.02.006.
17 Chiu SC, Hsu JK, Hu SC, et al. Molecular epidemiology of GⅠ.3 norovirus outbreaks from acute gastroenteritis surveillance system in Taiwan, 2015-2019[J]. Biomed Res Int, 2020, 2020: 4707538. PMID: 32104692. PMCID: PMC7040384. DOI: 10.1155/2020/4707538.
18 Lee B, Damon CF, Platts-Mills JA. Pediatric acute gastroenteritis associated with adenovirus 40/41 in low-income and middle-income countries[J]. Curr Opin Infect Dis, 2020, 33(5): 398-403. PMID: 32773498. PMCID: PMC8286627. DOI: 10.1097/QCO.0000000000000663.
19 沙比热木·托合塔木, 阿依古丽·伊尔哈力, 刘红斌, 等. 2010年乌鲁木齐市住院儿童病毒性腹泻监测结果分析[J]. 疾病监测, 2013, 28(6): 439-442. DOI: 10.3784/j.issn.1003-9961.2013.6.006.
20 李洁, 杨扬, 梁志超, 等. 北京地区五岁以下儿童病毒性急性胃肠炎病原构成与临床特征分析[J]. 中华预防医学杂志, 2020, 54(10): 1104-1110. PMID: 33115197. DOI: 10.3760/cma.j.cn112150-20191129-00892.
21 宫硕敏, 张宇耕, 闫绍宏, 等. 呼和浩特市2017-2019年5岁以下儿童病毒性腹泻病原学研究[J]. 中国病毒病杂志, 2020, 10(5): 342-345. DOI: 10.16505/j.2095-0136.2020.0046.
22 Kumthip K, Khamrin P, Ushijima H, et al. Enteric and non-enteric adenoviruses associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017[J]. PLoS One, 2019, 14(8): e0220263. PMID: 31369615. PMCID: PMC6675392. DOI: 10.1371/journal.pone.0220263.
23 周慧, 徐彬, 金威. 湖北地区学龄前儿童腹泻病毒性病原学情况调查分析[J]. 中国妇幼保健, 2018, 33(21): 4915-4918. DOI: 10.7620/zgfybj.j.issn.1001-4411.2018.21.39.
24 Lu LJ, Zhong HQ, Xu MH, et al. Molecular and epidemiological characterization of human adenovirus and classic human astrovirus in children with acute diarrhea in Shanghai, 2017-2018[J]. BMC Infect Dis, 2021, 21(1): 713. PMID: 34325664. PMCID: PMC8320412. DOI: 10.1186/s12879-021-06403-1.
25 Cortez V, Meliopoulos VA, Karlsson EA, et al. Astrovirus biology and pathogenesis[J]. Annu Rev Virol, 2017, 4(1): 327-348. PMID: 28715976. DOI: 10.1146/annurev-virology-101416-041742.
26 张会芳, 孔翔羽, 靳淼, 等. 2015年我国17省市5岁以下腹泻患儿星状病毒感染监测结果分析[J]. 国际病毒学杂志, 2017, 24(1): 5-8. DOI: 10.3760/cma.j.issn.1673-4092.2017.01.002.
27 Khamrin P, Thongprachum A, Okitsu S, et al. Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan[J]. J Med Virol, 2016, 88(2): 356-360. PMID: 26212039. DOI: 10.1002/jmv.24337.
28 Kumthip K, Khamrin P, Ushijima H, et al. Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011-2016[J]. Infect Genet Evol, 2018, 65: 373-379. PMID: 30153477. DOI: 10.1016/j.meegid.2018.08.024.
29 王森, 王永全, 苗元, 等. 2013年北京地区5岁以下儿童病毒性腹泻病原学研究[J]. 疾病监测, 2014, 29(5): 344-348. DOI: 10.3784/j.issn.1003-9961.2014.05.004.
30 Oka T, Wang QH, Katayama K, et al. Comprehensive review of human sapoviruses[J]. Clin Microbiol Rev, 2015, 28(1): 32-53. PMID: 25567221. PMCID: PMC4284302. DOI: 10.1128/CMR.00011-14.
31 周永康, 靳淼, 孔翔羽, 等. 2012-2014年中国八省区5岁以下腹泻儿童中札如病毒感染分析[J]. 国际病毒学杂志, 2016, 23(2): 81-84. DOI: 10.3760/cma.j.issn.1673-4092.2016.02.003.
32 Li W, Xiang WQ, Li CX, et al. Molecular epidemiology of rotavirus A and adenovirus among children with acute diarrhea in Hangzhou, China[J]. Gut Pathog, 2020, 12: 19. PMID: 32313556. PMCID: PMC7155314. DOI: 10.1186/s13099-020-00359-4.
33 李静, 邹文菁, 张婷, 等. 2018—2019年湖北地区5岁及以下儿童轮状病毒A组流行特征和病原学分析[J]. 公共卫生与预防医学, 2021, 32(4): 50-54. DOI: 10.3969/j.issn.1006-2483.2021.04.011.
34 Zhao LF, Shi XH, Meng DQ, et al. Prevalence and genotype distribution of group A rotavirus circulating in Shanxi Province, China during 2015-2019[J]. BMC Infect Dis, 2021, 21(1): 94. PMID: 33478417. PMCID: PMC7818068. DOI: 10.1186/s12879-021-05795-4.
35 包林, 艾静, 付建光, 等. 2015-2019年苏州市5岁以下儿童轮状病毒腹泻流行病学特征分析[J]. 国际病毒学杂志, 2021, 28(2): 111-115. DOI: 10.3760/cma.j.issn.1673-4092.2021.02.006.
36 Cho SR, Chae SJ, Jung S, et al. Trends in acute viral gastroenteritis among children aged ≤5 years through the national surveillance system in South Korea, 2013-2019[J]. J Med Virol, 2021, 93(8): 4875-4882. PMID: 33219526. PMCID: PMC8360024. DOI: 10.1002/jmv.26685.
37 Yuzhakov A, Yuzhakova K, Kulikova N, et al. Prevalence and genetic diversity of group A rotavirus genotypes in Moscow (2019-2020)[J]. Pathogens, 2021, 10(6): 674. PMID: 34070814. PMCID: PMC8228337. DOI: 10.3390/pathogens10060674.
38 Mohanty P, Kumar D, Mansingh A, et al. Rotavirus gastroenteritis hospitalizations among under-five children in Bhubaneswar, Odisha, India[J]. Indian J Pediatr, 2021, 88(Suppl 1): 53-58. PMID: 33417182. DOI: 10.1007/s12098-020-03607-2.
39 袁影, 赵艳玲, 孙利伟, 等. 吉林省小儿轮状病毒急性腹泻的病原学及临床特征分析[J]. 中国实验诊断学, 2018, 22(7): 1229-1230. DOI: 10.3969/j.issn.1007-4287.2018.07.049.
40 张佳莹, 田祎, 高志勇, 等. 亚洲轮状病毒腹泻流行病学特征[J]. 国际病毒学杂志, 2021, 28(2): 166-169. DOI: 10.3760/cma.j.issn.1673-4092.2021.02.018.
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