Influence of E-cadherin methylation on prognosis in children with acute lymphoblastic leukemia

QI Feng-Qi; HAN Wei; YAN Jing; XIN Cong; LI Yan; GUO Lei; WANG Wen-Peng; GAO Ji-Zhao

doi:10.7499/j.issn.1008-8830.2208018

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Chinese Journal of Contemporary Pediatrics ›› 2023, Vol. 25 ›› Issue (1) : 46-50. DOI: 10.7499/j.issn.1008-8830.2208018

CLINICAL RESEARCH

Influence of E-cadherin methylation on prognosis in children with acute lymphoblastic leukemia

QI Feng-Qi, HAN Wei, YAN Jing, XIN Cong, LI Yan, GUO Lei, WANG Wen-Peng, GAO Ji-Zhao

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Abstract

Objective To study the significance of E-cadherin and the association between E-cadherin methylation status and prognosis in children with acute lymphoblastic leukemia (ALL) by examining the mRNA and protein expression of E-cadherin and its gene methylation status in bone marrow mononuclear cells of children with ALL. Methods The samples of 5 mL bone marrow blood were collected from 42 children with ALL who were diagnosed for the first time at diagnosis (pre-treatment group) and on day 33 of induction chemotherapy (post-treatment group). RT-qPCR, Western blot, and methylation-specific PCR were used to measure the mRNA and protein expression of E-cadherin and the methylation level of the E-cadherin gene. The changes in each index after induction chemotherapy were compared. Results The mRNA and protein expression levels of E-cadherin in the post-treatment group were significantly higher than those in the pre-treatment group (P<0.05), while the positive rate of E-cadherin gene methylation in the post-treatment group was significantly lower than that in the pre-treatment group (P<0.05). At the end of the test, the children with negative methylation had significantly higher overall survival rate and event-free survival rate than those with positive methylation (P<0.05). Conclusions E-cadherin expression is associated with the development of ALL in children, and its decreased expression and increased methylation level may indicate a poor prognosis.

Key words

Acute lymphoblastic leukemia / E-cadherin / Methylation / Prognosis / Child

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QI Feng-Qi, HAN Wei, YAN Jing, XIN Cong, LI Yan, GUO Lei, WANG Wen-Peng, GAO Ji-Zhao. Influence of E-cadherin methylation on prognosis in children with acute lymphoblastic leukemia[J]. Chinese Journal of Contemporary Pediatrics. 2023, 25(1): 46-50 https://doi.org/10.7499/j.issn.1008-8830.2208018

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