Objective To study the effect of breastfeeding on immune function in infants with human cytomegalovirus (HCMV) infection. Methods A retrospective analysis was performed on the medical data of 135 infants with HCMV infection who were admitted to Children's Hospital Affiliated to Zhengzhou University from January 2021 to May 2022, and all these infants received breastfeeding. According to the results of breast milk HCMV-DNA testing, the infants were divided into two groups: breast milk HCMV positive (n=78) and breast milk HCMV negative (n=57). According to the median breast milk HCMV-DNA load, the infants in the breast milk HCMV positive group were further divided into two subgroups: high viral load and low viral load (n=39 each). Related indicators were compared between the breast milk positive and negative HCMV groups and between the breast milk high viral load and low viral load subgroups, including the percentages of peripheral blood lymphocyte subsets (CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, and CD19+ B cells), CD4+/CD8+ ratio, IgG, IgM, IgA, and urine HCMV-DNA load. Results There were no significant differences in the percentages of CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, and CD19+ B cells, CD4+/CD8+ ratio, IgG, IgM, IgA, and urine HCMV-DNA load between the breast milk HCMV positive and HCMV negative groups, as well as between the breast milk high viral load and low viral load subgroups (P>0.05). Conclusions Breastfeeding with HCMV does not affect the immune function of infants with HCMV infection.
Key words
Cytomegalovirus /
Breast milk /
Immune function /
Viral load /
Infant
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References
1 Martí-Carreras J, Maes P. Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges[J]. Virus Genes, 2019, 55(2): 138-164. PMID: 30604286. PMCID: PMC6458973. DOI: 10.1007/s11262-018-1627-3.
2 王淑珍, 孙文君. 不同喂养方式和分娩方式对出生胎龄≥32周新生儿/婴儿巨细胞病毒感染的影响以及感染结局[J]. 中国妇幼保健, 2018, 33(17): 3942-3945. DOI: 10.7620/zgfybj.j.issn.1001-4411.2018.17.33.
3 G?tting J, Lazar K, Suárez NM, et al. Human cytomegalovirus genome diversity in longitudinally collected breast milk samples[J]. Front Cell Infect Microbiol, 2021, 11: 664247. PMID: 33937103. PMCID: PMC8085339. DOI: 10.3389/fcimb.2021.664247.
4 冉丽红, 包蕾. 早产儿经母乳获得性巨细胞病毒感染的研究现状[J]. 中国当代儿科杂志, 2016, 18(10): 931-936. PMID: 27751206. PMCID: PMC7389540. DOI: 10.7499/j.issn.1008-8830.2016.10.003.
5 Berry R, Watson GM, Jonjic S, et al. Modulation of innate and adaptive immunity by cytomegaloviruses[J]. Nat Rev Immunol, 2020, 20(2): 113-127. PMID: 31666730. DOI: 10.1038/s41577-019-0225-5.
6 韦爱萍, 宋雅琴, 彭薇, 等. 人巨细胞病毒感染机体细胞免疫学机制的研究进展[J]. 医学综述, 2022, 28(7): 1308-1315. DOI: 10.3969/j.issn.1006-2084.2022.07.011.
7 吴伟平, 金晶, 曲春生, 等. 人巨细胞病毒感染婴儿肝功能相关指标分析[J]. 中国卫生检验杂志, 2017, 27(6): 827-829.
8 Ibrahim S, Siddiqui AA, Siddiqui AR, et al. Sociodemographic factors associated with IgG and IgM seroprevalence for human cytomegalovirus infection in adult populations of Pakistan: a seroprevalence survey[J]. BMC Public Health, 2016, 16(1): 1112. PMID: 27770770. PMCID: PMC5075404. DOI: 10.1186/s12889-016-3772-8.
9 丁光伟, 段淑芬, 高春耕, 等. 更昔洛韦联合还原型谷胱甘肽对巨细胞病毒性肝炎患者肝功能及Th1/Th2平衡的影响[J]. 中西医结合肝病杂志, 2021, 31(5): 424-427. DOI: 10.3969/j.issn.1005-0264.2021.05.011.
10 Fisher RA. Cytomegalovirus infection and disease in the new era of immunosuppression following solid organ transplantation[J]. Transpl Infect Dis, 2009, 11(3): 195-202. PMID: 19228345. DOI: 10.1111/j.1399-3062.2009.00372.x.
11 Einsele H, Roosnek E, Rufer N, et al. Infusion of cytomegalovirus (CMV)-specific T cells for the treatment of CMV infection not responding to antiviral chemotherapy[J]. Blood, 2002, 99(11): 3916-3922. PMID: 12010789. DOI: 10.1182/blood.v99.11.3916.
12 Macesic N, Langsford D, Nicholls K, et al. Adoptive T cell immunotherapy for treatment of ganciclovir-resistant cytomegalovirus disease in a renal transplant recipient[J]. Am J Transplant, 2015, 15(3): 827-832. PMID: 25648555. DOI: 10.1111/ajt.13023.
13 Armistead B, Jiang Y, Carlson M, et al. Spike-specific T cells are enriched in breastmilk following SARS-CoV-2 mRNA vaccination[J]. medRxiv[Preprint]. (2022-09-28) [2022-10-09]. PMID: 36203549. PMCID: PMC9536058. DOI: 10.1101/2021.12.03.21267036.
14 Godhia ML, Patel N. Colostrum—its composition, benefits as a nutraceutical—a review[J]. Curr Res Nutr Food Sci, 2013, 1(1): 37-47. DOI: 10.12944/CRNFSJ.1.1.04.
15 Moylan DC, Pati SK, Ross SA, et al. Breast milk human cytomegalovirus (CMV) viral load and the establishment of breast milk CMV-pp65-specific CD8 T cells in human CMV infected mothers[J]. J Infect Dis, 2017, 216(9): 1176-1179. PMID: 28968907. PMCID: PMC5853445. DOI: 10.1093/infdis/jix457.
16 Rabe T, Lazar K, Cambronero C, et al. Human cytomegalovirus (HCMV) reactivation in the mammary gland induces a proinflammatory cytokine shift in breast milk[J]. Microorganisms, 2020, 8(2): 289. PMID: 32093317. PMCID: PMC7074878. DOI: 10.3390/microorganisms8020289.
17 Lokossou GAG, Kouakanou L, Schumacher A, et al. Human breast milk: from food to active immune response with disease protection in infants and mothers[J]. Front Immunol, 2022, 13: 849012. PMID: 35450064. PMCID: PMC9016618. DOI: 10.3389/fimmu.2022.849012.
18 中华医学会儿科学分会感染学组, 全国儿科临床病毒感染协作组, 《中华儿科杂志》编辑委员会. 儿童巨细胞病毒性疾病诊断和防治的建议[J]. 中华儿科杂志, 2012, 50(4): 290-292. DOI: 10.3760/cma.j.issn.0578-1310.2012.04.013.
19 王淑珍, 孙文君. 不同喂养方式和分娩方式对出生胎龄≥32周新生儿/婴儿巨细胞病毒感染的影响以及感染结局[J].中国妇幼保健, 2018, 33(17): 3942-3945. DOI:10.7620/zgfybj.j.issn.1001-4411.2018.17.33.
20 Antona D, Lepoutre A, Fonteneau L, et al. Seroprevalence of cytomegalovirus infection in France in 2010[J]. Epidemiol Infect, 2017, 145(7): 1471-1478. PMID: 28166842. PMCID: PMC9203352. DOI: 10.1017/S0950268817000103.
21 Wang S, Wang T, Zhang W, et al. Cohort study on maternal cytomegalovirus seroprevalence and prevalence and clinical manifestations of congenital infection in China[J]. Medicine (Baltimore), 2017, 96(5): e6007. PMID: 28151899. PMCID: PMC5293462. DOI: 10.1097/MD.0000000000006007.
22 俞惠民. 早产儿母乳喂养与获得性巨细胞病毒感染[J]. 中华新生儿科杂志, 2020, 35(3): 161-163. DOI:10.3760/cma.j.issn.2096-2932.2020.03.001.
23 刘胜, 何杰书. 母婴喂养方式与分娩方式对婴儿巨细胞感染的影响情况分析[J]. 智慧健康, 2022, 8(5): 79-81. DOI: 10.19335/j.cnki.2096-1219.2022.05.025.
24 张琳, 王淮燕, 梅涛, 等. 母乳喂养对婴儿唾液巨细胞病毒载量的影响[J]. 中华实用儿科临床杂志, 2014, 29(10): 751-753. DOI: 10.3760/j.issn.2095-428X.2014.10.008.
25 杨洋, 王春晖, 王宝西, 等. 不同喂养方式对先天性巨细胞病毒感染新生儿病毒载量的影响[J]. 海南医学, 2020, 31(16): 2105-2107. DOI: 10.3969/j.issn.1003-6350.2020.16.018.
26 Lazar K, Kussmann T, Pawelec G, et al. Immunomonitoring of human breast milk cells during HCMV-reactivation[J]. Front Immunol, 2021, 12: 723010. PMID: 34566980. PMCID: PMC8462275. DOI: 10.3389/fimmu.2021.723010.
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