Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells

JIANG Wen, SHI Ding-Hua, HE Yan-Juan, CHEN Chun-Yuan

Chinese Journal of Contemporary Pediatrics ›› 2023, Vol. 25 ›› Issue (5) : 521-526.

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Chinese Journal of Contemporary Pediatrics ›› 2023, Vol. 25 ›› Issue (5) : 521-526. DOI: 10.7499/j.issn.1008-8830.2301031
EXPERIMENTAL RESEARCH

Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells

  • JIANG Wen, SHI Ding-Hua, HE Yan-Juan, CHEN Chun-Yuan
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Abstract

Objective To study the effect of procalcitonin (PCT) on lipopolysaccharide (LPS)-induced expression of the pyroptosis-related proteins nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and caspase-1 in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were induced by LPS to establish a model of sepsis-induced inflammatory endothelial cell injury. The experiment was divided into two parts. In the first part, HUVECs were randomly divided into four groups: normal control, LPS (1 μg/mL), PCT (10 ng/mL), and LPS+PCT (n=3 each). In the second part, HUVECs were randomly grouped: normal control, LPS, and LPS+PCT of different concentrations (0.1, 1, 10, and 100 ng/mL) (n=3 each). Quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NLRP3 and caspase-1 in each group. Results In the first experiment: compared with the normal control group, the PCT, LPS, and LPS+PCT groups had significantly upregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05); compared with the LPS group, the LPS+PCT group had significantly downregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05). In the second experiment: compared with those in the LPS group, the mRNA and protein expression levels of NLRP3 and caspase-1 in the LPS+PCT of different concentrations groups were significantly downregulated in a concentration-dependent manner (P<0.05). Conclusions LPS can promote the expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs, while PCT can inhibit the LPS-induced expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs in a concentration-dependent manner.

Key words

Sepsis / Procalcitonin / Pyroptosis / Nucleotide-binding oligomerization domain-like receptor protein 3 / Caspase-1 / Human umbilical vein endothelial cell

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JIANG Wen, SHI Ding-Hua, HE Yan-Juan, CHEN Chun-Yuan. Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells[J]. Chinese Journal of Contemporary Pediatrics. 2023, 25(5): 521-526 https://doi.org/10.7499/j.issn.1008-8830.2301031

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