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CJCP  2023, Vol. 25 Issue (6): 579-586    DOI: 10.7499/j.issn.1008-8830.2301086
TOPIC OF KAWASAKI DISEASE Current Issue| Next Issue| Archive| Adv Search  | 
Role and mechanism of platelet-derived growth factor BB in thrombocytosis in Kawasaki disease
SHEN Xi-Wei, TANG Zhi-Yuan, SHEN Xian-Juan, ZHAO Jian-Mei
Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China
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Abstract  Objective To study the role and mechanism of platelet-derived growth factor BB (PDGF-BB) on platelet production in Kawasaki disease (KD) mice and human megakaryocytic Dami cells through in vitro and in vivo experiments. Methods ELISA was used to measure the expression of PDGF in the serum of 40 children with KD and 40 healthy children. C57BL/6 mice were used to establish a model of KD and were then randomly divided into a normal group, a KD group, and an imatinib group (30 mice in each group). Routine blood test was performed for each group, and the expression of PDGF-BB, megakaryocyte colony forming unit (CFU-MK), and the megakaryocyte marker CD41 were measured. CCK-8, flow cytometry, quantitative real-time PCR, and Western blot were used to analyze the role and mechanism of PDGF-BB in platelet production in Dami cells. Results PDGF-BB was highly expressed in the serum of KD children (P<0.001). The KD group had a higher expression level of PDGF-BB in serum (P<0.05) and significant increases in the expression of CFU-MK and CD41 (P<0.001), and the imatinib group had significant reductions in the expression of CFU-MK and CD41 (P<0.001). In vitro experiments showed that PDGF-BB promoted Dami cell proliferation, platelet production, mRNA expression of PDGFR-β, and protein expression of p-Akt (P<0.05). Compared with the PDGF-BB group, the combination group (PDGF-BB 25 ng/mL + imatinib 20 μmol/L) had significantly lower levels of platelet production, mRNA expression of PDGFR-β, and protein expression of p-Akt (P<0.05). Conclusions PDGF-BB may promote megakaryocyte proliferation, differentiation, and platelet production by binding to PDGFR-β and activating the PI3K/Akt pathway, and the PDGFR-β inhibitor imatinib can reduce platelet production, which provides a new strategy for the treatment of thrombocytosis in KD.
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SHEN Xi-Wei
TANG Zhi-Yuan
SHEN Xian-Juan
ZHAO Jian-Mei
Key wordsKawasaki disease      Thrombocytosis      Platelet-derived growth factor BB      Dami cell      Child      Mouse     
Received: 01 February 2023     
Corresponding Authors: Zhao J-M,E-mail:2687084388@qq.com     E-mail: 2687084388@qq.com
Cite this article:   
SHEN Xi-Wei,TANG Zhi-Yuan,SHEN Xian-Juan et al. Role and mechanism of platelet-derived growth factor BB in thrombocytosis in Kawasaki disease[J]. CJCP, 2023, 25(6): 579-586.
SHEN Xi-Wei,TANG Zhi-Yuan,SHEN Xian-Juan et al. Role and mechanism of platelet-derived growth factor BB in thrombocytosis in Kawasaki disease[J]. CJCP, 2023, 25(6): 579-586.
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