Acetylcholine level changes in neonatal rats with cerebral hypoxia and ischemia
HUANG Yu-Chun, ZHANG Li, PAN Li-Li, HAN Yu-Kun
Department of Pediatrics, Second Teaching Hospital, China Medical University, Shenyang 110003
Abstract Objective To study the long-term effect of cerebral hypoxia and ischemia (HI) on the central acetylcholine (Ach) system and its treatments in neonatal rats. Methods Neonatal rats (7 days after birth) were subjected to permanent left common carotid artery ligation followed by 90 min of hypoxia with 10% oxygen at 36 ℃. The Ach level was measured immediately after HI (Group 1) and 14 days after HI (Group 2) using basic hydroxylamine chromatometry. In Group 3, basic fibroblast growth factor (bFGF) was injected (4μg ip) for 7 days immediately after HI. bFGF was given in the same way 3 days after HI in Group 4. The Ach level was measured 2 weeks after HI in Groups 3 and 4. Results The Ach level in the left globe decreased significantly immediately after HI (P<0.05). It did not return to normal until 14 days after HI. bFGF did not change the decreased Ach level obviously. Conclusions HI can damage the central Ach system of neonatal rat, moreover, the abnormality can persist for a long time. The bFGF has no protective effect on the damages of the Ach system .
Key words :
Hypoxia
Ischemia
Brain damage
Ach
Neonatal rat
bFGF
Cite this article:
HUANG Yu-Chun,ZHANG Li,PAN Li-Li et al. Acetylcholine level changes in neonatal rats with cerebral hypoxia and ischemia[J]. 中国当代儿科杂志, 1999, 1(3): 143-145.
HUANG Yu-Chun,ZHANG Li,PAN Li-Li et al. Acetylcholine level changes in neonatal rats with cerebral hypoxia and ischemia[J]. CJCP, 1999, 1(3): 143-145.
URL:
http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y1999/V1/I3/143
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