OBJECTIVE:  To introduce a simple, rapid and stable method for the determination of plasma acylation stimulating protein (ASP) and to explore the value of plasma ASP determination. METHODS: Sixty-three simple obese children and 98 healthy controls were included in this study. The ELISA technique was employed to determine the plasma ASP concentration. RESULTS: The best linearity of test was between 0.5-10 ng/mL. The intra-assay coefficient of variation (CV) was 8.17% and inter-assay CV, 9.72%. The average plasma ASP concentration in healthy children was 69.14±25.58 nM and 95.64±36.24 nM in obese children (P<0.001). Plasma ASP was positively correlated with body mass index in both healthy and obese children (r=0.2883 and 0.337, respectively, both P<0.01). CONCLUSIONS: Plasma ASP can be quantified by the ELISA method. Plasma ASP determination may be recommended as a new method for lipid metabolism determination in children.