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Effects of glutamine on extracellular signal regulated kinase and p38MAPK expression in the kidney in young rats with endotoxemia
ZHOU Ping, TUN Yu-Bin, ZHAO Cheng-Guang, TENG Xu, LI Li, LI Bing
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (04) : 301-305.
PDF(1769 KB)
PDF(1769 KB)
Effects of glutamine on extracellular signal regulated kinase and p38MAPK expression in the kidney in young rats with endotoxemia
OBJECTIVE: Glutamine has protective effects against renal injuries. This study was designed to explore the possible mechanism underlying the protections by examining the effects of glutamine on extracellular signal regulated kinase (ERK) and p38MAPK expression in the kidney in rats with endotoxemia. METHODS: One hundred and twenty-one 18-day-old Wistar rats were randomly injected with LPS (4 mg/kg; n=55), LPS (4 mg/kg)+glutamine (1 mL/kg) (n=55), or normal saline (control group; n=11). The two LPS groups were subdivided into five groups sacrificed at 2, 4, 6, 24 and 72 hrs after administration (n=11 each). ERK-2 and p38MAPK mRNA and protein expression in the kidney were measured by RT-PCR and immunohistrochemistry. RESULTS: Compared to the control group, the mRNA and protein expression of both ERK-2 and p38MAPK in the LPS group significantly increased 2, 4, 6, 24 and 72 hrs after administration (P<0.01), and reached a peak at 6 hrs (P<0.01). In the LPS+glutamine group, the trend of ERK-2 and p38MAPK expression was similar to the LPS group but their expressional levels were significantly lower than those in the LPS group at all time points (P<0.05 or 0.01). CONCLUSIONS: Both ERK-2 and p38MAPK expression increased in young rats with LPS-induced endotoxemia. Glutamine alleviates renal injuries possibly by decreasing the expression of both.［Chin J Contemp Pediatr, 2009, 11 (4):301-305］
Endotoxemia / Glutamine / Extracellular signal regulated kinase / p38MAPK / Rats
［1］ 段惠军， 李英，张涛，刘茂东，史永红.高糖对系膜细胞胞外调节蛋白激酶表达及细胞外基质合成的影响［J］. 中国中西医结合肾病杂志，2008，9（1）：47-49.
［2］韩梅，吴玉斌.谷氨酰胺对内毒素血症幼年大鼠肾脏细胞凋亡超微结构的影响［J］.中国现代医学杂志,2008，18(6):725-728.
［3］Diks SH, Richel DJ, Peppelenbosch MP. LPS signal transduction:the picture is becoming more complex［J］. Curr Top Med Chem, 2004, 4(11):1115-1126.
［4］Palsson-McDermott EM, O′Neill LA. Signal transduction by the lipopolysa-ccharide receptor, Toll-like receptor-4［J］. Immunology, 2004, 113(2):153-162.
［5］Kyriakis JM, Avruch J. Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation［J］. Physiol Rev, 2001, 81(2):807-869.
［6］Dunn KL, Espino PS, Drobic B, He S, Davie JR. The Ras-MAPK signal transduction pathway, cancer and chromatin remodeling［J］.Biochem Cell Biol, 2005, 83(1):1-14.
［7］刘茹, 郑军, 纪庆红.谷氨酰胺药物的临床应用进展［J］.中国药事, 2008, 22 （4）：346-347.
［8］Oehler R, Roth E. Regulative capacity of glutamine［J］.Curr Opin Clin Nutr Metab Care, 2003, 6（3）:277-282.
［9］Yi GF, Carroll JA, Allee GL, Gaines AM, Kendall DC, Usry JL, et al. Effect of glutamine and spray-dried plasma on growth perfomance, small intestinal morphology, and immune responses of Escherichia coli K88+-challenged weaned pigs［J］. J Anim Sci, 2005, 83(3):634-643.
［10］Evans ME, Jones DP, Ziegler TR. Glutamine prevents cytokine-induced apoptosis in human colonic epithelial cells［J］. J Nutr, 2003, 133(10):3065-3071.
［11］吴秀清，舒林华，孙梅，王虹，高红.谷氨酰胺对内毒素血症幼年大鼠小肠上皮细胞凋亡的作用及其抑制探讨［J］.中国当代儿科杂志，2006，8（6）：496-498.
［12］饶小平，朱绦绮，连惠红.谷氨酰胺对失血性休克幼兔肠屏障功能保护作用的研究［J］.中国当代儿科杂志，2006，8（1）：66-70.
［13］Blanc MC, Moinard C, Béziel A, Darguv S, Cynober L, De Bandt JP. Arginine and glutamine availability and macrophage functions in the obese insulin-resistant Zucker rat［J］. J Cell Physiol, 2005, 202(1):153-159.
［14］Cao Y, Kennedy R, Klimberg VS. Glutamine protects against doxorubicin-induced cardiotoxisity［J］. J Surg Res, 1999, 85(1):178-182.
