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Effects of hyperbaric oxygen on intrauterine hypoxic-ischemic brain damage in neonatal rats
CHEN Jing, CHEN Yan-Hui
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (05) : 380-384.
PDF(1253 KB)
PDF(1253 KB)
Effects of hyperbaric oxygen on intrauterine hypoxic-ischemic brain damage in neonatal rats
OBJECTIVE: The application and the efficacy of hyperbaric oxygen (HBO) in hypoxic-ischemic brain damage (HIBD) remain controversial. This study aimed to explore the effects of HBO on brain functional outcome and possible repair mechanisms in neonatal rats with intrauterine HIBD in aspects of the number of survived neurons and the central nervous electrophysiological conduction velocity. METHODS: A rat model of intrauterine HIBD was prepared. Subjects were divided into four groups at random: HIBD, HBO-treated HIBD group, normal control and HBO-treated normal control. After 24 hrs of the operation, the two HBO-treated groups received HBO treatment (0.02 MPa, 1 hr/d) for 14 days. When the rats were 4 weeks old, the electrophysiological changes in the central nervous system (CNS) were observed by brainstem auditory evoked potential (BAEP) for assessing brain function. Hematoxylin and eosin (HE) staining and Nissl's stainting were employed to observe the pathological change and the number of neurons in the hippocampus. RESULTS: The peak latency of waves II and IV and the interpeak latency of waves I-IV in the HBO-treated HIBD group were shortened compared with those in the untreated HIBD group (P<0.05). HE staining displayed that the pathological injuries in the hippocampus were alleviated in the HBO-treated HIBD group when compared with the untreated HIBD group. Nissl's staining showed that survived neurons in the HBO-treated HIBD group were more than the untreated HIBD group (P<0.05). The HBO-treated control group showed increased survived neurons compared with the untreated control group (P<0.05). CONCLUSIONS: Early HBO treatment might improve brain functional outcome through increasing synaptic transmission efficiency, improving central nervous electrophysiological conduction velocity and reducing neuron death in neonatal rats with intrauterine HIBD.［Chin J Contemp Pediatr, 2009, 11 (5):380-384］
Hypoxic-ischemic brain damage / Hyperbaric oxygen / Brainstem auditory evoked potential / Neuron death / Neonatal rats
［1］刘美娜，庄思齐，张红宇，覃肇源，李晓瑜.早期高压氧治疗对缺氧缺血性脑损伤新生大鼠的远期保护作用［J］.中国当代儿科杂志, 2006, 8(3):216-220.
［2］Li YH, Gong PL. Neuroprotective effects of dauricine against apoptosis induced by transient focal cerebral ischaemia in rats via a mitochondrial pathway［J］. Clin Exp Pharmacol Physiol, 2007, 34(3):177-184.
［3］Xiao N, Li S, Zhang X, Chen S. Effect of ephedrine on neuronal plasticity of hypoxic-ischemic brain damage in neonatal rats［J］.Neurosci Lett, 2008, 1435(2):99-102.
［4］Bjelke B, Andersson K, Ogren SO, Bolme P. Asphyctic lesion: proliferation of tyrosine hydroxylase-immunoreactive nerve cell bodies in the rat substantia nigra and functional changes in dopamine neurotransmission［J］.Brain Res, 1991, 543(1):1-9.
［5］Pazaiti A, Soubasi V, Spandou E, Karkavelas G, Georgiou T, Karalis P, et al. Evaluation of long-lasting sensorimotor consequences following neonatal hypoxic-ischemic brain injury in rats: the neuroprotective role of MgSO4［J］. Neonatology, 2008, 95(1):33-40.
［6］陈惠金，张忠德,周建德, 周泽汉，吴圣楣．脑缺氧缺血性损伤新生大鼠的脑形态学研究及病理量化评分［J］.上海医学, 2000, 23(11):682-684.
［7］Hou H, Grinberg O, Williams B, Grinberg S, Yu H, Alvarenga DL, et a1．The effect of oxygen therapy on brain damage and cerebral pO2 in transient focal cerebral ischemia in the rat［J］. Physiol Meas, 2007, 28(8):963-976.
［8］Li Z, Liu W, Kang Z, Lv S, Han C, Yun L, et al. Mechanism of hyperbaric oxygen preconditioning in neonatal hypoxia-ischemia rat model［J］.Brain Res, 2008, 1196:151-156.
［9］吴敬杰，刘敏，闫红涛，关重，王晔，李斌，等.闭合性颅脑损伤后大鼠BAEP及脑损伤的动态变化［J］. 法医学杂志, 2006, 22(5):324327.
［10］Culling FA著，孔庆雷译.组织病理学与组织化学技术手册［M］.北京:中国科技出版社, 1982, 92-102.
［11］王平宇.大白鼠中枢神经系统解剖学基础［M］.北京:人民卫生出版社，1986, 465-475．
［12］潘映辐.临床诱发电位学［M］.第2版.北京:人民卫生出版社, 2000, 258-293.
［13］Ostrowski RP, Graupner G, Titova E, Zhang J, Chiu J, Dach N, et al.The hyperbaric oxygen preconditioning-induced brain protection is mediated by a reduction of early apoptosis after transient global cerebral ischemia［J］.Neurobiol Dis, 2008, 29(1):1-13.
［14］巨容,封志纯.高压氧对缺氧缺血性脑损伤的保护作用及机制研究概述［J］.中国新生儿科杂志, 2007, 22(3):186-188.
［15］陈敏榕,陈燕惠,陈达光,陈敏.早期干预对缺氧缺血性脑损伤大鼠学习记忆能力及神经元凋亡的影响［J］.福建医科大学学报, 2004, 38(3):285-287.
［16］王晓莉，杨于嘉，王庆红，谢岷，余小河，刘沉涛，等.高压氧促HIBD新生大鼠内源性神经干细胞增殖过程中Wnt3蛋白的变化［J］.中国当代儿科杂志，2007, 9(3):241-246.
［17］王晓莉，杨于嘉，王庆红，余小河，谢岷，刘沉涛，等.不同时间窗高压氧治疗对HIBD新生大鼠白质损伤的影响［J］.中国当代儿科杂志，2007，9（4）：308-312.
［18］周北燕，卢光进，黄彦清，叶贞志，韩玉昆.不同压力高压氧治疗对新生儿HIE疗效观察［J］.中国当代儿科杂志，2008，10（2）：133-135.
