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Protective effect of ginsenoside Re on isoproterenol-induced triggered ventricular arrhythmia in rabbits
CHEN Cai-Xia, ZHANG Hong-Yan
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (05) : 384-388.
PDF(1725 KB)
PDF(1725 KB)
Protective effect of ginsenoside Re on isoproterenol-induced triggered ventricular arrhythmia in rabbits
OBJECTIVE: To study the anti-arrhythmic efficacy of ginsenoside Re (GSRe) and its protective effects against myocardial injuries in rabbits with isoproterenol-induced triggered ventricular arrhythmia (TVA). METHODS: TVA model was prepared by intravenous injections of isoproterenol at a constant speed of 5 mg/kg/min. When TVA appeared, rabbits were randomly injected with GSRe (5, 10 or 20 mg/kg), verapamil (0.4 mg/kg) or placebo. The duration of maintaining sinus rhythm was observed. Meanwhile, isoproterenol was continued to be injected at a constant speed of 5 mg/kg/min. After 1 hr of isoproterenol injection, the rabbits were sacrificed. Cardiac muscles in the cuspidate position of the left ventricle were sampled for optical microscopy and electron microscopy. RESULTS: GSRe and verapamil treatment restored sinus rhythm. The duration of sinus rhythm was 177.00±5.66 s within 3 minutes in the verapamil treatment group and was 177.83±5.31, 21.00±2.83 and 4.50±1.64 s, respectively, in the 20, 10 and 5 mg/kg GSRe treatment groups. Histopathologic examination demonstrated that GSRe treatment (20 and 10 mg/kg) alleviated myocardial injuries induced by TVA. CONCLUSIONS: GSRe has anti-arrhythmic efficacies and protective effects against myocardial injuries in rabbits with TVA. It may therefore be a possible therapy for TVA.［Chin J Contemp Pediatr, 2009, 11 (5):384-388］
Ginsenoside Re / Isoproterenol / Triggered ventricular arrhythmia / Rabbits
［1］Cho WC, Chung WS, Lee SK, Leung AW, Cheng CH, Yue KK.Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats［J］. Eur J Pharmacol, 2006, 550(1-3):173-179.
［2］Zhou XM, Cao YL, Dou DQ. Protective effect of ginsenoside Re against cerebralischenia/reperfusion damage in rats［J］. Biol Pharm Bull, 2006, 29(12):2502-2505.
［3］Lopez MV, Cuadrado MP, RuizPoveda OM, Del Fresno AM, Accame ME.Neuroprotective effect of individual ginsenosides on astrocytes primary culture［J］. Biochim Biophys Acta, 2007, 1770(9):1308-1316.
［4］Kim YS, Kim JJ, Cho KH, Jung WS, Moon SK, Park EK, et al. Biotransformation of ginsenoside Rb1, crocin, amygdalin, geniposide, puerarin, ginsenoside Re, hesperidin, poncirin, glycyrrhizin, and baicalin by human fecal microflora and its relation to cytotoxicity against tumor by human fecal microflora and its relation to cytotoxicity against tumor［J］. J Microbiol Biotechnol, 2008, 18(6):1109-1114.
［5］Chen LM, Zhou XM, Cao YL, Hu WX. Neuroprotection of ginsenoside Re in cerebral ischemia-reperfusion injury in rats［J］. J Asian Nat Prod Res, 2008, 10(5-6):439-445.
［6］Zhang Z, Li X, Lv W, Yang Y, Gao H, Yang J, et al. Ginsenoside Re reduces insulin resistance through inhibition of c-Jun NH2-terminal kinase and nuclear factor-kappaB［J］. Mol Endocrinol, 2008, 22(1):186-195.
［7］López MV, Cuadrado MP, Ruiz-Poveda OM, Del Fresno AM, Accame ME. Neuroprotective effect of individual ginsenosides on astrocytes primary culture［J］. Biochim Biophys Acta, 2007, 1770(9):1308-1316.
［8］Xie JT, Mehendale SR, Li X, Quigg R, Wang X, Wang CZ, et al. Anti-diabetic effect of ginsenoside Re in ob/ob mice［J］. Biochim Biophys Acta, 2005, 1740(3):319-325.
［9］Leung KW, Leung FP, Huang Y, Mak NK, Wong RN. Non-genomic effects of ginsenoside-Re in endothelial cells via glucocorticoid receptor［J］. FEBS Lett, 2007, 581(13):2423-2428.
［10］Igawa M, Aonuma K, Okamoto Y, Hiroe M, Hiraoka M, Isobe M. Anti-arrhythmic efficacy of nifekalant hydrochloride, a pure class III antiarrhythmic agent, in patients with healed myocardial infarction and inducible sustained ventricular tachycardia［J］. J Cardiovasc Pharmacol, 2002, 40(5):735-742.
［11］李才，王丽娟，赵志涛.灶性心肌坏死的定量组织学测定-逐区点测量法［J］.白求恩医科大学学报, 1988, 14（4）：314-317.
［12］Rona G.Catecholamine cardiotoxicity［J］. J Mol Cell Cardiol, 1985, 17(4):291-306.
［13］沈俊海，解春红，童美琴.300例小儿异位快速心律失常的诊断和治疗［J］.中国当代儿科杂志, 2003, 5(3):277-279.
［14］郑若玄，方三曼，李志明，张雄谋.蒲黄对大白鼠心律失常的预防作用［J］.中国中药杂志, 1993, 18（2）：108-110.
［15］Bartel S, Krause EG, Wallukat G, Karczewski P. New insights into beta2-adrenoceptor signaling in the adult rat heart［J］. Cardiovasc Res, 2003, 57(3):694-703.
［16］Tsuchida K, Watajima H. Cyclic AMP-mediated increase in L-type calcium current (ICa,L) by nitroglycerin in guinea-pig ventricular myocytes［J］. Cell Mol Biol (Noisy-le-grand), 2002,48(2):179-185.
［17］Pinelli A,Trivulzio S, Momasoni L, Brenna S, Bonacina E, Vignati S, et al. Myocardial infartion non-invasively induced in rabbits by administering isoproterenol and vasopression:protective effects exerted by verapamil［J］. Fundam Clin Pharmacol, 2004, 18(6):657-667.
［18］Sorbera C, Cohen M, Kuznetsov V, Dhakham S.Verapamil-sensitive left ventricular tachycardia in patients with coronary artery disease:clinical and electrophysiologic features consistent with triggered activity［J］. Heart Dis, 1999, 1(1)：2-7.
［19］AngLee MK, Moss J, Yuan CS. Herbal medicines and perioperative care［J］. JAMA, 2001, 286(2):208-216.
［20］Bai CX, Takahashi K, Masumiya H, Sawanobori T, Furukawa T. Nitric oxide-dependent modulation of the delayed rectifier K+ current and the L-type Ca2+ current by ginsenoside Re, an ingredient of Panax ginseng, in guinea-pig cardiomyocytes［J］. Br J Pharmacologgy, 2004, 142(3):567-575.
［21］Furukawa T, Bai CX, Kaihara A, Ozaki E, Kawano T, Nakaya Y, et al. Ginsenoside Re, a main phytosterol of Panax ginseng, activates cardiac potassium channels via a nongenomic pathway of sex hormones［J］. Mol Pharmacol, 2006, 70(6):1916-1924.
［22］Nakaya Y, Mawatari K, Takahashi A, Harada N, Hata A, Yasui S. The phytoestrogen ginsensoside Re activates potassium channels of vascular smooth muscle cells through PI3K/Akt and nitric oxide pathways［J］. J Med Invest, 2007, 54(3-4):381-384.
［23］Leung KW, Leung FP, Huang Y, Mak NK, Wong RN. Non-genomic effects of ginsenoside-Re in endothelial cells via glucocorticoid receptor［J］. FEBS Lett, 2007, 581(13):2423-2428.
