PDF(1044 KB)
Expression of heat shock protein 90 in peripheral blood mononuclear cells of children with myasthenia gravis
LIU Run-Ning, HUANG Zhi
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (06) : 453-456.
PDF(1044 KB)
PDF(1044 KB)
Expression of heat shock protein 90 in peripheral blood mononuclear cells of children with myasthenia gravis
OBJECTIVE: Myasthenia gravis is an autoimmunity disease and its pathogenesis has not been fully identified. Heat shock protein 90 (HSP90) shows an abnormal expression in other autoimmunity diseases. This study examined the mRNA expression of two isoforms of HSP90 in peripheral blood mononuclear cells (PBMC) and serum cortisol content in children with myasthenia gravis. METHODS: Thirty-six children with myasthenia gravis and 19 healthy children were enrolled. Serum cortisol content was measured by the chemiluminescence assay. The expression of HSP90α and HSP90β mRNA in PBMC was detected by the RT-PCR technique. RESULTS: The mRNA expression of HSP90α (0.7329±0.2120) and HSP90β (0.7193±0.2869) in children with myasthenia gravis was significantly higher than that in healthy controls (0.5574±0.2084 and 0.4892±0.2104 respectively) (P<0.01). Serum cortisol content (285.04±146.39 nmol/L) in children with myasthenia gravis was also higher than that in the healthy controls (196.25±64.52 nmol/L) (P<0.01). CONCLUSIONS: The high mRNA expression of HSP90α and HSP90β in PBMC might be associated with the development of myasthenia gravis. The high serum cortisol level indicates a high stress state or might be correlated to the glucocorticoid receptor abnormality in children with myasthenia gravis.［Chin J Contemp Pediatr, 2009, 11 (6):453-456］
Myasthenia gravis / Heat shock protein 90 / Cortisol / Child
［1］李久宏，韩世新，高奎斌.狼疮肾炎患者热休克蛋白90及亚型mRNA表达的研究［J］.中国麻风皮肤病杂志, 2005, 21(1):1-3.
［2］Somji S, Ann Sens M, Garrett SH, Gurel V, Todd JH, Sens DA. Expression of hsp 90 in the human kidney and in proximal tubule cells exposed to heat, sodium arsenite and cadmium chloride［J］. Toxicol Lett, 2002, 133(2-3):241-254.
［3］Murphy PJ, Morishima Y, Kovacs JJ, Yao TP, Pratt WB. Regulation of the dynamics of hsp90 action on the glucocorticoid receptor by acetylation/deacetylation of the chaperone［J］. Biological Chemistry, 2005, 280(40):33792-33799.
［4］欧阳涓，姜傥. 热休克蛋白90与类固醇激素受体功能的研究进展［J］.国外医学临床生物化学与检验学分册, 2005, 26(4)：217-219.
［5］钱小顺，朱元珏，许文兵，林耀广.哮喘患者外周血单个核细胞中热休克蛋白90和糖皮质激素受体mRNA的表达［J］.中华结核和呼吸杂志, 2001, 24(7)：425-428.
［6］谢琰臣，矫毓娟，许贤豪，王红，殷剑.重症肌无力患者外周血单个核细胞中糖皮质激素受体不同五型表达和意义［J］. 中华内科杂志, 2007, 46(1):56-59.
［7］李亚琴，曹兰芳.肾上腺糖皮质激素在幼年特发性关节炎中的应用进展［J］.中国当代儿科杂志, 2009, 11(3):240-242.
［8］Elbi C, Walker DA, Lewis M, Romero G, Sullivan WP. Molecular chaperones function as steroid receptor nuclear mobility factors［J］. Proc Natl Acad Sci USA, 2004, 101(9):2876-2881.
［9］Grad I, Picard D. The glucocorticoid responses are shaped by molecular chaperones［J］. Mol Cell Endocrinol, 2007, 275(1-2):2-12.
［10］Zhang X, Clark AF, Yorio T. Heat shock protein 90 is an essential molecular chaperone for nuclear transport of glucocorticoid receptor beta［J］. Invest Ophthalmol Vis Sci, 2006, 47(2):700-708.
