Effects of prenatal taurine on mRNA expression of PKA-CREB signal pathway and glial cell line-derived neurotrophic factor in fetal rat brains of intrauterine growth restriction

CHEN Hui; LI Jian; LIU Jing; LIU Li; LIU Na; SONG Yi-Zhi

PDF(1029 KB)

Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (12) : 923-926.

Effects of prenatal taurine on mRNA expression of PKA-CREB signal pathway and glial cell line-derived neurotrophic factor in fetal rat brains of intrauterine growth restriction

CHEN Hui, LI Jian, LIU Jing, LIU Li, LIU Na, SONG Yi-Zhi

Author information +

History +

Abstract

OBJECTIVE: This study examined the effects of prenatal application of taurine on mRNA expression of protein kinase A-cAMP response element binding protein (PKA-CREB) signal pathway and glial cell line-derived neurotrophic factor (GDNF) in fetal rat brains of intrauterine growth restriction (IUGR). METHODS: Pregnant rats were randomly divided into 4 groups: normal control, IUGR model, low-dose (100 mg/kg?d) and high-dose (300 mg/kg?d) taurine treatment IUGR (n=5 each). IUGR was induced by food restriction throughout pregnancy. PKA, CREB and GDNF mRNA expression in brains of newborn rats was detected by reverse transcription-polymerase chain reaction (RT-PCR). RETHODS: PKA, CREB and GDNF mRNA expression in the IUGR model group was significantly higher than that in the normal control group (P<0.05). Compared with the IUGR model group, mRNA expression of PKA and CREB in both the low-dose and high-dose taurine treatment groups increased significantly (P<0.05); GDNF mRNA expression in the high-dose taurine treatment group also increased significantly (P<0.01). CONCLUSIONS: Taurine can increase mRNA expression of PKA, CREB and GDNF in fetal rat brains of IUGR. This suggests that prenatal application of taurine may increase neurogenesis of the central nervous system and endogenous secretion of neurotrophic factors, thus providing neuroprotective effects. ［Chin J Contemp Pediatr, 2009, 11 (11):923-926］

Key words

Intrauterine growth restriction / Taurine / cAMP response element binding protein / Glial cell line-derived neurotrophic factor / Fetal rats

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHEN Hui, LI Jian, LIU Jing, LIU Li, LIU Na, SONG Yi-Zhi. Effects of prenatal taurine on mRNA expression of PKA-CREB signal pathway and glial cell line-derived neurotrophic factor in fetal rat brains of intrauterine growth restriction[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(12): 923-926

References

［1］Jacobsson B, Ahlin K, Francis A, Hagberg G, Hagberg H, Gardosi J. Cerebral palsy and restricted growth status at birth: population-based case-control study［J］. BJOG, 2008, 115(10):1250-1255.
［2］陈慧,刘敬.宫内生长受限对围产期脑发育的影响［J］.中华儿科杂志, 2009, 47(1):33-35.
［3］黄婷婷,丘小汕,杜敏联,沈振宇，柯志勇，赖峰.宫内生长迟缓大鼠脂肪组织肿瘤坏死因子αmRNA表达与胰岛素抵抗关系的研究［J］.中华儿科杂志,2005,43(1):39-42.
［4］李新娟,葛治国,李东亮,李庆岗,李明阳,秦宇,等.牛磺酸对大鼠脑损伤后认知功能及海马凋亡相关蛋白表达的影响［J］.新乡医学院学报, 2006, 23(6):569-571.
［5］Cetin I, Alvino G. Intrauterine growth restriction:implications for placental metabolism and transport. A review［J］. Placenta, 2009, 30(Suppl A):S77-S82.
［6］Samuelsen GB, Pakkenberg B, Bogdanovic N, Gundersen HJ, Larsen JF, Graem N, et al. Severe cell reduction in the future brain cortex in human growth-restricted fetuses and infants［J］. Am J Obstet Gynecol, 2007, 197(1):56.e1-7.
［7］Lodygensky GA, Seghier ML, Warfield SK, Tolsa CB, Sizonenko S, Lazeyras F, et al. Intrauterine growth restriction affects the preterm Infant′s hippocampus［J］. Pediatr Res, 2008, 63(4):438-443.
［8］刘丽,陈慧,刘娜,刘敬,景鹏,杨连雪.牛磺酸对宫内生长受限新生幼鼠大脑Bcl-2,Bax及Caspase-3 蛋白表达的影响［J］.实用儿科临床杂志,2009,24(2):109-111.
［9］Hannila SS,Filbin MT.The role of cyclic AMP signaling in promoting axonal regeneration after spinal cord injury［J］.Exp Neurol, 2008, 209(2):321-332.
［10］Kerschensteiner M, Meinl E, Hohlfeld R. Neuro-immune crosstalk in CNS diseases［J］.Results Probl Cell Differ, 2009, 158(3):1122-1132.
［11］Wood MD, Moore AM, Hunter DA, Tuffaha S, Borschel GH, Mackinnon SE, et al. Affinity-based release of glial-derived neurotrophic factor from fibrin matrices enhances sciatic nerve regeneration［J］. Acta Biomaterialia, 2009, 5(4):959-968.
［12］Kaneko N, Sawamoto K. Adult neurogenesis and its alteration under pathological conditions［J］.Neurosci Res, 2009, 63(3):155-164.
［13］Sandhu JK, Gardaneh M, Iwasiow R, Lanthier P, Gangaraju S, Ribecco-Lutkiewicz M, et al. Astrocyte-secreted GDNF and glutathione antioxidant system protect neurons against 6OHDA cytotoxicity［J］.Neurobiol Dis, 2009, 33(3):405-414.
［14］Saavedra A, Baltazar G, Duarte EP. Driving GDNF expression:The green and the red trafficlights［J］. Prog Neurobiol, 2008, 86(3):186-215.
［15］Tatli M, Guzel A, Kizil G, Kavak V, Yavuz M, Kizil M. Comparison of the effects of maternal protein malnutrition and intrauterine growth restriction on redox state of central nervous system in offspring rats［J］.Brain Res, 2007, 1156:21-30.
［16］Wu J, Kohno T, Georgiev SK, Ikoma M, Ishii H, Petrenko AB, et al. Taurine activates glycine and gamma-aminobutyric acid A receptors in rat substantia gelatinosa neurons［J］. Neuroreport, 2008, 19(3):333-337.
［17］del Olmo N, Handler A, Alvarez L, Bustamante J, Martin del Rio R, Solis JM. Taurine-induced synaptic potentiation and the late phase of longterm potentiation are related mechanistically［J］. Neuropharmacology, 2003, 44(1):26-39.
［18］Taranukhin AG, Taranukhina EY, Saransaari P, Djatchkova IM, Pelto-Huikko M, Oja SS. Taurine reduces caspase-8 and caspase-9 expression induced by ischemia in the mouse hypothalamic nuclei［J］.Amino Acids, 2008, 34(1):169-174.