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Expression of blood Toll-like receptors and cytokines in children with recurrent herpes simplex
TANG Jian-Ping, SUN Lei
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (9) : 722-724.
PDF(1070 KB)
PDF(1070 KB)
Expression of blood Toll-like receptors and cytokines in children with recurrent herpes simplex
OBJECTIVE: This study examined the expression of Toll-like receptors (TLR) in peripheral blood mononuclear cells (PBMC) and serum levels of IL-6, IL-10 and TNF-α in children with recurrent herpes simplex, in order to investigate the role of TLR2 and TLR9 in recurrent herpes simplex. METHODS: The expression of TLR2 and TLR9 in PBMC was examined by flow cytometer, and serum levels of IL-6, IL-10 and TNF-α were detected using ELISA in 22 children with recurrent herpes simplex and in 13 age-matched healthy volunteers. RESULTS: The expression of both TLR2 and TLR9 obviously increased in children with recurrent herpes simplex compared with that in healthy controls (P<0.01). Serum levels of IL-6 and IL-10 increased, while serum TNF-α levels decreased significantly in children with recurrent herpes simplex compared with those in healthy controls (P<0.01). There were positive correlations between TLR2 and TLR9 expression and serum IL-6 and IL-10 levels in children with recurrent herpes simplex (P<0.01). CONCLUSIONS: TLR2 and TLR9 in PBMC may participate in the recognition of herpes simplex virus and activate the signal pathway of TLR in children with recurrent herpes simplex. The production and release of IL-6 and IL-10 might be mediated by TLR2 and TLR9.［Chin J Contemp Pediatr, 2009, 11 (9):722-724］
Recurrent herpes simplex / Peripheral blood mononuclear cell / Toll-like receptor / Cytokine / Child Recurrent herpes simplex / Peripheral blood mononuclear cell / Toll-like receptor / Cytokine / Child
［1］徐文严．生殖器疱疹［M］.// 赵辨．临床皮肤病学．第3版．南京：江苏科学技术出版社，2003，541-546．
［2］Hochrein H, Schlatter B, O′Keeffe M, Wagner C, Schmitz F, Schiemann M,et al. Herpes simplex virus type-1 induces IFN-α production via Toll-like receptor 9-dependent and -independent pathways［J］. Proc Natl Acad Sci USA, 2004, 101(31):11416-11421.
［3］Lund J, Sato A, Akira S, Medzhitov R, Iwasaki A.Toll-like receptor 9-mediated recognition of herpes simplex virus-2 by plasmacytoid dendritic cells［J］. J Exp Med, 2003, 198(3):513-520.
［4］Sato A, Linehan M, Iwasaki A.Dual recognition of herpes simplex viruses by TLR2 and TLR9 in dendritic cells［J］. PNAS, 2006, 103 (46):17343-17348.
［5］Sergerie Y, Rivest S, Boivin G. Tumor necrosis factor-α and interleukin-1 beta play a critical role in the resistance against lethal herpes simplex virus encephalitis［J］. J Infect Dis, 2007, 196(6):853-860.
［6］Kurt-Jones EA, Chan M, Zhou S, Wang J, Reed G. Herpes simplex virus 1 interaction with Toll-like receptor 2 contributes to lethal encephalitis［J］. Proc Natl Acad Sci USA, 2004, 101(5):1315-1320.
［7］Gill N, Davies EJ, Ashkar AA. The role of toll-like receptor ligands/agonists in protection against genital HSV-2 infection［J］. Am J Reprod Immunol, 2008, 59(1):35-43.
［8］Hayashi K, Hooper LC, Chin MS, Nagineni CN, Detrick B, Hooks JJ. Herpes simplex virus 1 (HSV-1) DNA and immune complex (HSV-1human IgG) elicit vigorous interleukin 6 release from infected corneal cells via Toll-like receptors［J］. J Gen Virol, 2006, 87(Pt 8):2161-2169.
