Expression of regulatory T cells and Foxp3 gene in peripheral blood of children with aplastic anemia

WANG Xi-Ge; WANG Xiao-Ge; LUAN Bin; HU Ji-Ting

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (04) : 241-243.

CLINICAL RESEARCH

Expression of regulatory T cells and Foxp3 gene in peripheral blood of children with aplastic anemia

WANG Xi-Ge, WANG Xiao-Ge, LUAN Bin, HU Ji-Ting

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Abstract

OBJECTIVE: To investigate the levels of CD4+CD25+CD127low regulatory T cells (Tregs) and the expression of Foxp3 gene in peripheral blood of children with aplastic anemia (AA) and to study their roles in the pathogenesis of AA. METHODS: Twenty-one children with chronic AA, 9 with acute AA and 15 healthy children were enrolled. The proportion of CD4+CD25+ CD127low Tregs in CD4+ T cells was evaluated by flow cytometric analysis. The level of Foxp3 mRNA was ascertained by RT-PCR. RESULTS: The percentage of peripheral blood CD4+T cells and CD4+CD25+ and CD4+CD25+CD127low Tregs in CD4+T cells in both the acute and chronic AA groups was significantly lower than that in the normal control group (P﹤0.05). The acute AA group had more decreased CD4+ T cells and CD4+CD25+ and CD4+CD25+CD127low Tregs percentage compared with the CAA group (P<0.05). The expression of Foxp3 mRNA in peripheral blood decreased obviously in the acute AA group (0.47±0.08%) compared with that in the normal control (0.71±0.12%) and the CAA groups (0.68±0.14%) (P<0.05). CONCLUSIONS: The low expression of Tregs and Foxp3 mRNA in peripheral blood may be involved in pathogenesis of AA. The more decreased Tregs and Foxp3 mRNA expression in acute AA than chronic AA suggests their possible roles in the assessment of the severity of AA.［Chin J Contemp Pediatr, 2010, 12 (4):241-243］

Key words

Aplastic anemia / Regulatory T cell / Flow cytometry / Foxp3 / Child

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WANG Xi-Ge, WANG Xiao-Ge, LUAN Bin, HU Ji-Ting. Expression of regulatory T cells and Foxp3 gene in peripheral blood of children with aplastic anemia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(04): 241-243

References

［1］Brodsky RA, Jones RJ. Aplastic anemia［J］.Lancet, 2005, 365(9471):1647-1656.
［2］中华医学会儿科分会血液组,中华儿科杂志编委会. 小儿再生障碍性贫血的诊疗建议［J］. 中华儿科杂志, 2001, 39（7):422-423.
［3］Sakaguchi S, Sakaguchi N, Asano M, Itoh M, Toda M. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25): breakdown of a single mechanism of self-tolerance causes various autoimmune diseases［J］. J Immunol, 1995, 155(3):1151-1164.
［4］Costantino CM, Baecher-Allan CM, Hafler DA. Human regulatory T cells and autoimmunity［J］. Eur Immunol, 2008, 38(4):921-924.
［5］Sakaguchi S. The origin of FOXP3-expressing CD4+ reguatory T cells：thymus or periphery［J］．J Clin Invest, 2003, 112(9)：1310-1322．
［6］Solomou EE, Rezvani K, Mielke S, Malide D, Keyvanfar K, Visconte V, et al．Deficient CD4+CD25+FOXP3+T regulatory cells in acquired aplastie anemia［J］．Blood, 2007, 110(5):1603-1606．
［7］Chen J, Ellison FM, Eckhaus MA, Smith AL, Keyvanfar K, Calado RT, et al. Minor antigen H60-mediated aplastic anemia is ameliorated by immunosuppression and the infusion of regulatory T cells［J］. J Immunol, 2007, 178(7):4159-4168．
［8］Hartigan-O′Connor DJ, Poon C, Sinclair E, McCune JM. Human CD4+regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells［J］. J Immunol Methods, 2007, 319(1-2): 41-52.
［9］Fontenot JD, Rudensky AY. A well adapted regulatory contriveance: regulatory T cell development and the forkhead family transcription factor Foxp3［J］. NAT Immunol, 2005, 6(4):331-337.