PDF(1107 KB)
Expression of microRNA in neonatal rats with hypoxic-ischemic brain damage
PENG Tao, JIA Yan-Jie, WEN Quan-Qing, GUAN Wen-Juan, ZHAO Er-Yi, ZHANG Bo-Ai
Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (05) : 373-376.
PDF(1107 KB)
PDF(1107 KB)
Expression of microRNA in neonatal rats with hypoxic-ischemic brain damage
OBJECTIVE: To study the changes of microRNA expression in cortex tissues in neonatal rats with hypoxic-ischemic brain damage（HIBD）and the possible roles of microRNA in the pathogenesis of HIBD.MethodsRat HIBD model was prepared. The cortex tissues were obtained 14 days after the HIBD event. The microRNA expression profiles were measured using microRNA microarray. Expression of 9 microRNAs (miR-126,-26a,-674-5p,-21,-25,-290, miR-124,-125b-5p and microRNA-9a) was determined by quantitative real-time PCR. RESULTS: he results of microRNA expression profiles indicated that 27 pieces of microRNA were up-regulated more than 2 folds and 60 pieces were down-regulated more than 2 folds compared with the normal control group. The results of the 9 microRNAs detected by quantitative real-time PCR were consistent with those detected by microRNA microarray. CONCLUSIONS: HIBD rats have significant changes in microRNA expression, suggesting that microRNA expression may play important roles in the pathogenesis of HIBD.［Chin J Contemp Pediatr, 2010, 12 (5):373-376］
［1］Barrett RD, Bennet L, Davidson J, Dean JM, George S, Emerald BS, et al. Destruction and reconstruction: hypoxia and the developing brain［J］.Birth Defects Res C Embryo Today, 2007, 81（3）:163-176.
［2］Chuang JC, Jones PA. Epigenetics and microRNAs［J］. Pediatr Res, 2007, 61（5 Pt 2）:24R-29R.
［3］Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of posttranscriptional regulation by microRNAs: are the answers in sight?［J］. Nat Rev Genet, 2008, 9(2):102-114.
［4］Rice JE, Vannucci RC, Brierley JB. The inflluence lf immaturity of hypoxic-ischemic brain damage in the rat［J］.Ann Neurol, 1981, 9(2):131-134.
［5］刘丽旭，杨于嘉. 高压氧治疗新生大鼠缺氧缺血性脑损伤量效及时效关系［J］.中国当代儿科杂志, 2001, 3(4):355-358.
［6］文全庆, 贾延劼, 王明闯, 赵二义, 王留东, 张博爱,等.大鼠脑缺血急性期脑组织miRNA的表达变化［J］.重庆医科大学学报, 2008, 33(S1):23-26.
［7］Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, et al. A mammalian microRNA expression atlas based on small RNA library sequencing［J］. Cell, 2007, 129(7):1401-1414.
［8］Gao FB. Posttranscriptional control of neuronal development by microRNA networks［J］. Trends Neurosci, 2008, 31(1):20-26.
［9］Jeyaseelan K, Lim KY, Armugam A. MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion ［J］. Stroke, 2008, 39(3):959-966.
［10］Dharap A, Bowen K, Place R, Li LC, Vemuganti R. Transient focal ischemia induces extensive temporal changes in rat cerebral microRNAome［J］. J Cereb Blood Flow Metab, 2009, 29(4):675-687.
［11］Kulshreshtha R, Davuluri RV, Calin GA, Ivan M. A microRNA component of the hypoxic response［J］. Cell Death Differ, 2008, 15(4):667-671.
［12］van Solingen C, Seghers L, Bijkerk R, Duijs JM, Roeten MK, van Oeveren-Rietdijk AM, et al. AntagomiR-mediated silencing of endothelial cell specific microRNA-126 impairs ischemia-induced angiogenesis［J］. J Cell Mol Med, 2009, 13(8A):1577-1585.
［13］Urbich C, Kuehbacher A, Dimmeler S. Role of microRNAs in vascular diseases, inflammation, and angiogenesis［J］. Cardiovasc Res, 2008, 79(4):581-588.
［14］Cheng LC, Pastrana E, Tavazoie M, Doetsch F. miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche［J］. Nat Neurosci, 2009, 12 (4):399-408.
［15］Le MT, Xie H, Zhou B, Chia PH, Rizk P, Um M, et al. MicroRNA-125b promotes neuronal differentiation in human cells by repressing multiple targets［J］. Mol Cell Biol, 2009, 29(19):5290-5305.
［16］Denli AM, Cao X, Gage FH. miR-9 and TLX: chasing tails in neural stem cells［J］. Nat Struct Mol Biol, 2009, 16(4):346-347.
