Decreased circulating endothelial progenitor cell function: relationship with serum concentrations of high-sensitivity C-reactive protein in children with Kawasaki disease

XU Ming-Guo, MEN Li-Na, WANG Hai-Xia, Zu Ying, ZHAO Chun-Yu, ZHAO Xia, CAI Hua-Bo, MENG Xiang-Chun, WANG Tao

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (07) : 513-517.

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (07) : 513-517.
CLINICAL RESEARCH

Decreased circulating endothelial progenitor cell function: relationship with serum concentrations of high-sensitivity C-reactive protein in children with Kawasaki disease

  • XU Ming-Guo, MEN Li-Na, WANG Hai-Xia, Zu Ying, ZHAO Chun-Yu, ZHAO Xia, CAI Hua-Bo, MENG Xiang-Chun, WANG Tao
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Abstract

OBJECTIVE: To study the function of circulating endothelial progenitor cells and its relationship with serum concentrations of high-sensitivity C-reactive protein (Hs-CRP) in children with Kawasaki disease. METHODS: Ten children with Kawasaki disease and ten healthy children as a control group were enrolled. The peripheral mononuclear cells were induced into endothelial progenitor cells using Dulbecco’s Modified Eagle Medium containing vascular endothelial growth factor and basic fibroblast growth factor. The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells were assessed by MTT methods, modified Boyden chamber methods and cell culture plate adhesion method, respectively. The concentrations of serum Hs-CRP were measured by latex enhanced turbidimetric immunoassay. RESULTS: The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells in the Kawasaki disease group were significantly lower than those in the control group (P<0.01). The serum concentrations of Hs-CRP in the Kawasaki disease group were significantly higher than those in the control group (87.1±30.2 mg/L vs 5.3±3.4 mg/L; P<0.01). The function of circulating endothelial progenitor cells was negatively correlated with serum concentrations of Hs-CRP in the Kawasaki disease group. CONCLUSIONS: The function of circulating endothelial progenitor cells is decreased in children with Kawasaki disease, which may be associated with the abnormal expression of inflammatory mediators.[Chin J Contemp Pediatr, 2010, 12 (7):513-517]

Key words

Kawasaki disease / Endothelial progenitor cell / Endothelial cell / High-sensitivity C-reactive protein / Child

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XU Ming-Guo, MEN Li-Na, WANG Hai-Xia, Zu Ying, ZHAO Chun-Yu, ZHAO Xia, CAI Hua-Bo, MENG Xiang-Chun, WANG Tao. Decreased circulating endothelial progenitor cell function: relationship with serum concentrations of high-sensitivity C-reactive protein in children with Kawasaki disease[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(07): 513-517

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