Impact of neonatal bacillus Calmette-Guerin vaccination on lung Th17 cells and IL-17 in murine asthma model

CHEN Wei-Chao; LIU En-Mei; DENG Yu; HE Yun; CHEN Jie-Hua; LI Xin; LIU Wei

PDF(1119 KB)

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (08) : 647-650.

EXPERIMENTAL RESEARCH

Impact of neonatal bacillus Calmette-Guerin vaccination on lung Th17 cells and IL-17 in murine asthma model

CHEN Wei-Chao, LIU En-Mei, DENG Yu, HE Yun, CHEN Jie-Hua, LI Xin, LIU Wei

Author information +

History +

Abstract

OBJECTIVE: To study the impact of neonatal bacillus Calmette-Guerin(BCG) vaccination on lung Th17 cells and IL-17 in murine asthma model. METHODS: Neonatal BALB/c mice were divided into three groups: control, OVA and BCG/OVA groups. BCG was administerd in the BCG/OVA group on postnatal day 2 or 3. Except the control group, the mice in the other two groups were sensitized and undergone OVA challenge. Inflammatory cell numbers and morphological identification of leucocytes in bronchoalveolar lavage fluid (BALF) were measured by light microscopy. Cytokine IFN-γ and IL-17 levels in BALF were measured using ELISA. The percentage of lung Th17 cells were assayed by flow cytometry. RESULTS: There was significantly larger number of total cells, lymphocytes, eosinophils and neutrophils in BALF in the OVA and BCG/OVA groups compared with the control group. The number or percentage of those cells in the BCG/OVA group was lower than that in the OVA group. The level of IL-17 in BALF was significantly higher in the OVA and the BCG/OVA groups compared with the control group, while the level of IFN-γ was lower. The OVA group had higher level of IL-17 than the BCG/OVA group. The mice in the OVA and the BCG/OVA groups had a higher percentage of Th17 cells in lungs compared with the control group, but there were no significant differences in the percentage of Th17 cells between the OVA and the BCG/OVA groups. CONCLUSIONS: Th17 cells and IL-17 play roles in the pathogenesis of asthma. BCG vaccination can reduce the level of IL-17 in BALF and the reduced IL-17 may be mainly from other IL-17-producing cells in the lungs, not Th17 cells. ［Chin J Contemp Pediatr, 2010, 12 (8):650-653］

Key words

Bacillus Calmette-Guerin / Asthma / Th17 cell / IL-17 / Neonatal mice

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHEN Wei-Chao, LIU En-Mei, DENG Yu, HE Yun, CHEN Jie-Hua, LI Xin, LIU Wei. Impact of neonatal bacillus Calmette-Guerin vaccination on lung Th17 cells and IL-17 in murine asthma model[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(08): 647-650

References

［1］Nakae S, Komiyama Y, Nambu A, Sudo K, Iwase M, Homma I, et al. Antigen-specific T cell sensitization is impaired in IL-17-deficient mice, causing suppression of allergic cellular and humoral responses［J］. Immunity, 2002, 17(3):375-387.
［2］Wilson RH, Whitehead GS, Nakano H, Free ME, Kolls JK, Cook DN. Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness［J］. Am J Respir Crit Care Med, 2009, 180(8):720-730.
［3］Shirakawa T, Enomoto T, Shimazu S, Hopkin JM. The inverse association between tuberculin responses and atopic disorder［J］. Science, 1997, 275(5296):77-79.
［4］Hopfenspirger MT, Agrawal DK. Airway hyperresponsiveness, late allergic response, and eosinophilia are reversed with mycobacterial antigens in ovalbumin-presensitized mice［J］. J Immunol, 2002, 168(5):2516-2522.
［5］Yang X, Fan Y, Wang S, Han X, Yang J, Bilenki L, et al. Mycobacterial infection inhibits established allergic inflammatory responses via alteration of cytokine production and vascular cell adhesion molecule-1 expression［J］. Immunology, 2002, 105(3):336-343.
［6］李睿，刘恩梅，杨锡强，王莉佳.卡介苗新生期接种和呼吸道合胞病毒感染对小鼠实验性哮喘的联合作用［J］.中华儿科杂志，2006，44（6）：420-424.
［7］Lee J, Reinke EK, Zozulya AL, Sandor M, Fabry Z. Mycobacterium bovis bacille Calmette-Guérin infection in the CNS suppresses experimental autoimmune encephalomyelitis and Th17 responses in an IFN-gamma-independent manner［J］. J Immunol, 2008, 181(9):6201-6212.
［8］Infante-Duarte C, Horton HF, Byrne MC, Kamradt T. Microbial lipopeptides induce the production of IL-17 in Th cells［J］. J Immunol, 2000, 165(11):6107-6115.
［9］Bettelli E, Korn T, Oukka M, Kuchroo VK. Induction and effector functions of T(H)17 cells［J］. Nature, 2008, 453(7198):1051-1057.
［10］Hung LY, Velichko S, Huang F, Thai P, Wu R. Regulation of airway innate and adaptive immune responses: the IL-17 paradigm［J］. Crit Rev Immunol, 2008, 28(4):269-279.
［11］Hashimoto T, Akiyama K, Kobayashi N, Mori A. Comparison of IL-17 production by helper T cells among atopic and nonatopic asthmatics and control subjects［J］. Int Arch Allergy Immunol, 2005, 137 (Suppl 1):51-54.
［12］Hellings PW, Kasran A, Liu Z, Vandekerckhove P, Wuyts A, Overbergh L, et al. Interleukin-17 orchestrates the granulocyte influx into airways after allergen inhalation in a mouse model of allergic asthma［J］. Am J Respir Cell Mol Biol, 2003, 28(1):42-50.
［13］Cruz A, Khader SA, Torrado E, Fraga A, Pearl JE, Pedrosa J, et al. Cutting edge: IFN-gamma regulates the induction and expansion of IL-17producing CD4 T cells during mycobacterial infection［J］. J Immunol, 2006, 177(3):1416-1420.
［14］Song C, Luo L, Lei Z, Li B, Liang Z, Liu G, et al. IL-17-producing alveolar macrophages mediate allergic lung inflammation related to asthma［J］. J Immunol, 2008, 181(9): 6117-6124.