Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura

REN Shao-Min; ZHANG Chun-Xia; LI Wei-Cai

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (1) : 29-31.

CLINICAL RESEARCH

Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura

REN Shao-Min, ZHANG Chun-Xia, LI Wei-Cai

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Abstract

OBJECTIVE: To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-γ and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP. METHODS: Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-γ and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls. RESULTS: Blood T-bet mRNA expression and IFN-γ levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (P<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (P<0.01). CONCLUSIONS: The high expression of T-bet and IFN-γ and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.［Chin J Contemp Pediatr, 2010, 12 (1):29-31］

Key words

Idiopathic thrombocytopenic purpura / Th1/Th2；Transcriptional factor / Cytokine / Child

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REN Shao-Min, ZHANG Chun-Xia, LI Wei-Cai. Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(1): 29-31

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