不同潮气量通气启动新生鼠肺纤维化的特点

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Abstract OBJECTIVE: To study the changes of collagen synthesis following mechanical ventilation with different tidal volume and the possible mechanism. METHODS: Twenty-four neonatal Sprague-Dawley rats were randomized to hyperventilation (tidal volume 25 mL/kg), conventional ventilation (tidal volume 10 mL/kg) and no mechanical ventilation (control group) (n=8 each group). They were sacrificed 5 hrs after ventilation. Left lung samples were used for histopathologic examinations and the detection of connective tissue growth factor (CTGF) expression by immunohistochemistry. Right lung samples were used for the detection of expression of procollagenⅢ mRNA(PcolⅢ mRNA), cysteinyl leukotriene mRNA（CysLT1 mRNA）and CTGF mRNA by PCR. RESULTS: The severity of lung injury and fibrosis increased significantly with the increasing tidal volume compared with the control group. Lung CTGF mRNA expression in the hyperventilation group was significantly higher than that in the control group (P<0.05). Lung PcolⅢ mRNA and CysLT1 mRNA levels increased with the increasing tidal volume when compared with the control group. The differences in PcolⅢ mRNA and CysLT1 mRNA levels between groups were significant (P<0.05). There was a positive correlation between lung PcolⅢ mRNA expression and the severity of lung injury (r=0.78，P<0.01). CTGF and CysLT levels were positively correlated with PcolⅢ expression (r＝0.59，0.86，P<0.01). CONCLUSIONS: Mechanical ventilation using different tidal volume leads to different severities of lung injury, followed by the occurrence of lung fibrosis. The degree of lung fibrosis is consistent with the severity of lung injury. CysLT and CTGF may be involved in the development of lung fibrosis.［Chin J Contemp Pediatr, 2010, 12 (10):799-803］

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	Articles by authors
	HUANG Jin-Jie
	WU Ben-Qing
	DING Lu
	CHEN Li

Key words： Mechanical ventilation Lung fibrosis ProcollagenⅢ Cysteinyl leukotriene Connective tissue growth factor Neonatal rats

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R-33

Cite this article:

HUANG Jin-Jie,WU Ben-Qing,DING Lu et al. Lung fibrosis induced by mechanical ventilation with different tidal volume in neonatal rats[J]. 中国当代儿科杂志, 2010, 12(10): 799-803.

HUANG Jin-Jie,WU Ben-Qing,DING Lu et al. Lung fibrosis induced by mechanical ventilation with different tidal volume in neonatal rats[J]. CJCP, 2010, 12(10): 799-803.

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http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y2010/V12/I10/799

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