Effects of inhaled budesonide on the bronchial-pulmonary pathology and expression of thymic stromal lymphopoietin in lung tissues in asthmatic rats

LIU Hui, PAN Jia-Hua, LOU Wan-Ling, HE Jin-Gen

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (10) : 816-819.

PDF(1570 KB)
PDF(1570 KB)
Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (10) : 816-819.
EXPERIMENTAL RESEARCH

Effects of inhaled budesonide on the bronchial-pulmonary pathology and expression of thymic stromal lymphopoietin in lung tissues in asthmatic rats

  • LIU Hui, PAN Jia-Hua, LOU Wan-Ling, HE Jin-Gen
Author information +
History +

Abstract

OBJECTIVE: Thymic stromal lymphopoietin (TSLP) plays an important role in initiating dendritic cell mediated allergic inflammation. This study was designed to examine the effects of inhaled budesonide on TSLP expression in the lung tissues and on the bronchial-pulmonary pathology in asthmatic rats. METHODS: Thirty-two female Sprague-Dawley rats were sensitized and challenged with inhaled ovabumin (OVA) to induce asthma. The asthmatic rats were randomly divided into 2 groups on the 22nd day of OVA challenge: a budesonide treatment group that received inhaled budesonide at 0.32 mg/kg daily for 7 days and an asthma control group that received inhaled 0.9% normal saline for 7 days. TSLP expression in the lung tissues was measured by Western blot and fluorescent-immunohistochemistry 29 and 36 days after OVA challenge. Bronchial-pulmonary pathological changes were evaluated by hematoxylin & eosin and periodic acid-schiff staining. RESULTS: Budesonide treatment alleviated airway inflammation when compared with the asthma control group 29 days after OVA challenge. However, the airway inflammatory reactions were aggravated in the budesonide treatment group 36 days after OVA challenge (7 days after budesonide discontinuance). TSLP expression in the lung tissues was significantly lower in the budesonide treatment group than that in the asthma control group both 29 and 36 days after OVA challenge (P<0.05). CONCLUSIONS: Inhaled budesonide can inhibit the TSLP expression in the lung tissues and alleviate lung inflammatory reactions in asthmatic rats, but there is end-of-dose failure.[Chin J Contemp Pediatr, 2010, 12 (10):816-819]

Key words

Asthma / Thymic stromal lymphopoietin / Budesonid / Rats

Cite this article

Download Citations
LIU Hui, PAN Jia-Hua, LOU Wan-Ling, HE Jin-Gen. Effects of inhaled budesonide on the bronchial-pulmonary pathology and expression of thymic stromal lymphopoietin in lung tissues in asthmatic rats[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(10): 816-819

References

[1]Soumelis V, Reche PA, Kanzler H, Yuan W, Edward G, Homey B, et al. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP[J].Nat Immunol, 2002,3(7):673-680.
[2]张瑞珊,赵源,谭娴玲.普米克令舒吸入佐治小儿毛细支气管炎疗效观察[J].中国实用儿科杂志,2001,16(5):289-291.
[3]叶辉,应小明,童夏生,陈利中,赵蓓.布地奈德对哮喘大鼠中性粒细胞诱导型一氧化氮合酶表达的影响[J].中国临床药理学与治疗学,2009,14(9):1008-1011.
[4]Mauad T, Silva LF, Santos MA, Grinberg L, Bernardi FD, Martins MA, et al. Abnormal alveolar attachments with decreased elastic fiber content in distal lung in fatal asthma[J].Am J Respir Crit Care Med, 2004, 170 (8): 857-862.
[5]Gibson PG, Saltos N, Fakes K. Acute anti-inflammatory effects of inhaled budesonide in asthma: a randomized controlled trial[J]. Am J Respir Crit Care Med, 2001, 163(1): 32-36.
[6]Wempe JB, Tammeling EP, Koeter GH, Hakansson L, Venge P, Postma DS. Blood eosinophil numbers and activity during 24 hours:effects of treatment with budesonide and bambuterol[J]. J Allergy Clin Immunol, 1992, 90 (5):757-765.
[7]Boschetto P, Rogers DF, Fabbri LM, Barnes PJ. Corticosteroid inhibition of airway microvascular leakage[J]. Am Rev Respir Dis, 1991, 143(3): 605-609.
[8]杜以明,薛玉文,葛秀利.哮喘患者长期吸入糖皮质激素气道炎症的变化[J].山东医药,2004,44(25):23-24.
[9]Lee HC, Ziegler SF.Inducible expression of the proallergic cytokine thymic stromal lymphopoietin in airway epithelial cells is controlled by NFkappaB[J]. Proc Natl Acad Sci U S A, 2007,104(3):914-919.
[10]Cato AC, Wade E. Molecular mechanisms of anti-inflammatory action of glucocorticoids[J]. Bioessays, 1996, 18(5): 371-378.

PDF(1570 KB)

Accesses

Citation

Detail

Sections
Recommended

/