Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells

LI Zhong-Xia; JIA Xiu-Hong; LI Jian-Chang; HAN Lin

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (11) : 897-901.

EXPERIMENTAL RESEARCH

Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells

LI Zhong-Xia, JIA Xiu-Hong, LI Jian-Chang, HAN Lin

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Abstract

OBJECTIVE: To study the protective effect of bone marrow stromal cells (BMSCs) upon childhood leukemia cells and the influence of VLA-4 antibody in vitro on leukemia cell apoptosis. METHODS: BMSCs from children with acute leukemia-were isolated by human lymphocyte separation medium. BMSCs (adherent) and leukemia cells (suspended) were cultured in vitro. This study included four groups: leukemia cells alone (control), leukemia cells+BMSCs, leukemia cells+BMSCs supernatant and leukemia cells+BMSCs+VLA-4 antibody. The apoptosis rate of leukemia cells in the four groups was determined by Annexin Ⅴ-FITC double-labeled flow cytometry. The expression of survivin and bcl-2 genes in leukemia cells was ascertained by RT-PCR. RESULTS: The apoptosis rate of leukemia cells in the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups was lower than that in the control group (P<0.05). Compared with the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups, the apoptosis rate of leukemia cells in the VLA-4 antibody group increased significantly (P<0.05). In the VLA-4 antibody group, the apoptosis rate of leukemia cells increased with prolonged culture time. There were significant differences in the apoptosis rate between 12 hrs and 24 hrs after VLA-4 antibody treatment (P<0.01). The expression of survivin and bcl-2 genes in leukemia cells from the VLA-4 antibody groups was reduced compared with that from the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups (P<0.05). CONCLUSIONS: BMSCs play protective roles on leukemia cells. VLA-4 antibody can block the adhesion between BMSCs and leukemia cells and promote leukemia cell apoptosis.［Chin J Contemp Pediatr, 2010, 12 (11):897-901］

Key words

Luekemia / Bone marrow stromal cell / VLA-4 antibody / Apoptosis / Leukemia cell

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LI Zhong-Xia, JIA Xiu-Hong, LI Jian-Chang, HAN Lin. Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(11): 897-901

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