CJCP
  中文版
  English Version
 
中国当代儿科杂志  2010, Vol. 12 Issue (11): 897-901    DOI:
EXPERIMENTAL RESEARCH Current Issue| Next Issue| Archive| Adv Search  | 
Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells
LI Zhong-Xia, JIA Xiu-Hong, LI Jian-Chang, HAN Lin
Department of Pediatrics, Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong 256603, China. Email:jiaxiuhong001@163.com
Download: PDF (1146 KB)  
Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract  OBJECTIVE: To study the protective effect of bone marrow stromal cells (BMSCs) upon childhood leukemia cells and the influence of VLA-4 antibody in vitro on leukemia cell apoptosis. METHODS: BMSCs from children with acute leukemia-were isolated by human lymphocyte separation medium. BMSCs (adherent) and leukemia cells (suspended) were cultured in vitro. This study included four groups: leukemia cells alone (control), leukemia cells+BMSCs, leukemia cells+BMSCs supernatant and leukemia cells+BMSCs+VLA-4 antibody. The apoptosis rate of leukemia cells in the four groups was determined by Annexin Ⅴ-FITC double-labeled flow cytometry. The expression of survivin and bcl-2 genes in leukemia cells was ascertained by RT-PCR. RESULTS: The apoptosis rate of leukemia cells in the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups was lower than that in the control group (P<0.05). Compared with the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups, the apoptosis rate of leukemia cells in the VLA-4 antibody group increased significantly (P<0.05). In the VLA-4 antibody group, the apoptosis rate of leukemia cells increased with prolonged culture time. There were significant differences in the apoptosis rate between 12 hrs and 24 hrs after VLA-4 antibody treatment (P<0.01). The expression of survivin and bcl-2 genes in leukemia cells from the VLA-4 antibody groups was reduced compared with that from the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups (P<0.05). CONCLUSIONS: BMSCs play protective roles on leukemia cells. VLA-4 antibody can block the adhesion between BMSCs and leukemia cells and promote leukemia cell apoptosis.[Chin J Contemp Pediatr, 2010, 12 (11):897-901]
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
LI Zhong-Xia
JIA Xiu-Hong
LI Jian-Chang
HAN Lin
Key wordsLuekemia      Bone marrow stromal cell      VLA-4 antibody      Apoptosis      Leukemia cell     
    
PACS: R733.7  
Cite this article:   
LI Zhong-Xia,JIA Xiu-Hong,LI Jian-Chang et al. Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells[J]. 中国当代儿科杂志, 2010, 12(11): 897-901.
LI Zhong-Xia,JIA Xiu-Hong,LI Jian-Chang et al. Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells[J]. CJCP, 2010, 12(11): 897-901.
URL:  
http://www.zgddek.com/EN/     OR     http://www.zgddek.com/EN/Y2010/V12/I11/897
Copyright © 2004-2005 Chinese Journal Of Contemporary Pediatrics All rights reserved.
Addr: 87 Xiangya Road, Changsha, Hunan, 410008, PRC
Tel: 0731-84327402 Fax: 0731-84327922 E-mail: cjcp1999@csu.edu.cn