ID4 methylation patterns in childhood T line and B line lymphocytic leukemia

HU Hong-Bo; HU Qun

PDF(936 KB)

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (12) : 940-942.

CLINICAL RESEARCH

ID4 methylation patterns in childhood T line and B line lymphocytic leukemia

HU Hong-Bo, HU Qun

Author information +

History +

Abstract

OBJECTIVE: To study the relationship of methylation of inhibitor of DNA binding 4 (ID4) gene core promoter region with childhood T line, B line and T/B acute lymphocytic leukemia (ALL). METHODS: Methylation-specific polymerase chain reaction (MS-PCR) was used to detect the methylation status of ID4 promoter region in 18 children with newly-diagnosed ALL (2 cases of T-ALL, 13 cases of B-ALL and 3 cases of T/B-ALL). Thirty-four hospitalized children with non-tumor disease served as the control group. RESULTS: The complete methylation rate of ID4 gene promoter region (15/18, 83%) was significantly higher than the partial methylation rate (3/18, 17%) in the 18 ALL children (P<0.05). The complete methylation rate of ID4 gene promoter region in children with T-ALL, B-ALL and T/B-ALL (50%, 85% and 100% respectively) was significantly higher than that in the control group (18%; P<0.05). In contrast, the partial methylation rate and non-methylation rate in the three ALL groups were significantly lower than those in the control group (P<0.05). There were no statistically significant differences in the methylation patterns among the B-ALL, T-ALL and T/B-ALL cases. CONCLUSIONS: The methylation of ID4 promoter region may be related to the pathogenesis of childhood ALL. The methylation patterns of ID4 promoter region are identical in BALL, TALL and T/BALL.［Chin J Contemp Pediatr, 2010, 12 (12):940-942］

Key words

Leukemia / Methylation / Inhibitor of DNA binding 4 / Child

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

HU Hong-Bo, HU Qun. ID4 methylation patterns in childhood T line and B line lymphocytic leukemia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(12): 940-942

References

［1］Li Y, Nagai H, Ohno T, Yuge M, Hatano S, Ito E, et al. Aberrant DNA methylation of p57(KIP2) gene in the promoter region in lymphoid malignancies of B-cell phenotype［J］. Blood, 2002, 100(7): 2572-2577.
［2］胡洪玻,胡群,刘爱国,张耀东，王冠玲.白血病ID4基因核心启动子区甲基化及其表达［J］. 肿瘤,2009,29(9):874-878.
［3］中华医学会儿科分会血液学组. 儿童急性淋巴细胞白血病诊疗建议(第三次修订草案)［J］. 中华儿科杂志，2006，44 (5)：392-395.
［4］Pagliuca A, Cannada-Bartoli P, Lania L. A role for Sp and helix-loop-helix transcription factors in the regulation of the human Id4 gene promoter activity［J］. J Biol Chem, 1998, 273(13): 7668-7674.
［5］Uhm KO, Lee ES, Lee YM, Kim HS, Park YN, Park SH. Aberrant promoter CpG islands methylation of tumor suppressor genes in cholangiocarcinoma［J］. Oncol Res, 2008, 17(4): 151-157.
［6］Borinstein SC, Conerly M, Dzieciatkowski S, Biswas S, Washington MK, Trobridge P, et al. Aberrant DNA methylation occurs in colon neoplasms arising in the azoxymethane colon cancer model［J］. Mol Carcinog, 2010, 49(1): 94-103.
［7］Tsunoda S, Smith E, De Young NJ, Wang X, Tian ZQ, Liu JF, et al. Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma［J］. Oncol Rep, 2009, 21(4): 1067-1073.
［8］Roldán G, Delgado L, Musé LM. Tumoral expression of BRCA1, estrogen receptor alpha and ID4 protein in patients with sporadic breast cancer［J］. Cancer Biol Ther, 2006, 5(5): 505-510.
［9］Noetzel E, Veeck J, Niederacher D, Galm O, Horn F, Hartmann A, et al. Promoter methylation-associated loss of ID4 expression is a marker of tumour recurrence in human breast cancer［J］. BMC Cancer, 2008, 8: 154.
［10］Yuen HF, Chua CW, Chan YP, Wong YC, Wang X, Chan KW. Id proteins expression in prostate cancer: high-level expression of Id-4 in primary prostate cancer is associated with development of metastases［J］. Mod Pathol, 2006, 19(7): 931-941.
［11］Asirvatham AJ, Schmidt MA, Chaudhary J. Non-redundant inhibitor of differentiation (Id) gene expression and function in human prostate epithelial cells［J］. Prostate, 2006, 66(9): 921-935.
［12］Carey JP, Asirvatham AJ, Galm O, Ghogomu TA, Chaudhary J. Inhibitor of differentiation 4 (Id4) is a potential tumor suppressor in prostate cancer［J］. BMC Cancer, 2009, 9:173.
［13］Yu L, Liu C, Vandeusen J, Becknell B,Dai Z,Wu YZ, et al. Global assessment of promoter methylation in a mouse model of cancer identifies ID4 as a putative tumor-suppressor gene in human leukemia［J］. Nat Genet, 2005, 37（3）: 265-274.
［14］Hagiwara K, Nagai H, Li Y, Ohashi H, Hotta T, Saito H. Frequent DNA methylation but not mutation of the ID4 gene in malignant lymphoma［J］. J Clin Exp Hematop, 2007, 47(1): 15-18.
［15］赵瑜, 于力, 王全顺, 李红华, 薄剑, 窦丽萍. DNA结合抑制因子4基因甲基化状态检验在急性白血病中的意义［J］. 中华内科杂志, 2006, 45(7): 576-578.
［16］Uhm KO, Lee ES, Lee YM, Park JS, Kim SJ, Kim BS, et al. Differential methylation pattern of ID4, SFRP1, and SHP1 between acute myeloid leukemia and chronic myeloid leukemia［J］. J Korean Med Sci, 2009, 24(3): 493-497.