A comparative study on three models of co-culture of neurons and astrocytes

GUO Hui; MA Jiao; TONG Yu; QU Yi; MU De-Zhi; MAO Meng

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (12) : 984-987.

EXPERIMENTAL RESEARCH

A comparative study on three models of co-culture of neurons and astrocytes

GUO Hui, MA Jiao, TONG Yu, QU Yi, MU De-Zhi, MAO Meng

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Abstract

OBJECTIVE: To find a better method for harvesting highly purified neurons by comparing three methods used for co-culture of neurons and astrocytes. METHODS: The co-culture models of neurons and astrocytes were established by primary culture, Banker's co-culture method or Transwell cell-culture inserts. The neurons and astrocytes cultured in vitro were from neonatal rats. RESULTS: The highly purified neurons were not harvested by primary culture because the neurons and astrocytes grew on the same cover slip and it was difficult to control the growth velocity of astrocytes. The highly purified neurons were harvested by Banker's co-culture method or the method using Transwell cell-culture inserts, but the procedure of the former was more complicated than that of the later. CONCLUSIONS: The culture method using Transwell cell-culture inserts is recommended for the establishment of the co-culture system of neurons and astrocytes.［Chin J Contemp Pediatr, 2010, 12 (12):984-987］

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Co-culture / Neuron / Astrocyte / Neonatal rats

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GUO Hui, MA Jiao, TONG Yu, QU Yi, MU De-Zhi, MAO Meng. A comparative study on three models of co-culture of neurons and astrocytes[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(12): 984-987

References

［1］何家全，蔡文琴，张可成，杨忠，肖茂磊. 大鼠中枢神经系统大胶质细胞对神经元轴突生长影响的离体实验观察［J］. 第三军医大学学报，1999，21(9): 630-632.
［2］白雪涛. 利用胶质细胞滋养层进行分离式原代神经元培养［J］. 卫生研究，2002, 31(4): 299-300.
［3］Carbone DL, Popichak KA, Moreno JA, Safe S, Tjalkens RB. Suppression of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced nitric-oxide synthase 2 expression in astrocytes by a novel diindolylmethane analog protects striatal neurons against apoptosis［J］. Mol Pharmacol, 2009, 75(1):35-43.
［4］Madrigal JL, Leza JC, Polak P, Kalinin S, Feinstein DL. Astrocyte-derived MCP-1 mediates neuroprotective effects of noradrenaline［J］. J Neurosci, 2009, 29(1):263-267.
［5］Carlson NG. Neuroprotection of cultured cortical neurons mediated by the cyclooxygenase-2 inhibitor APHS can be reversed by a prostanoid［J］. J Neurosci Res, 2003, 71(1): 79-88.
［6］Yin Z, Milatovic D, Aschner JL, Syversen T, Rocha JBT, Souza DO, et al. Methylmercury induces oxidative injury, alterations in permeability and glutamine transport in cultured astrocytes［J］. Brain Res, 2007, 1131(1): 1-10.
［7］刘利，林志国，沈红，车彦军，张帆，白云龙，等.神经元和胶质细胞共培养方法的建立［J］. 中华神经外科疾病研究杂志，2006, 5(4): 317-320.
［8］申军现，金卫林，鞠躬. 低密度体外培养大鼠海马神经元的形态学观察［J］. 第四军医大学学报，2003,24(6):489-491.
［9］Fujita T, Tozaki-Saitoh H, Inoue K. P2Y1 receptor signaling enhances neuroprotection by astrocytes against oxidative stress via IL-6 release in hippocampal cultures［J］. Glia, 2009, 57(3): 244-257.
［10］郭琴，毛萌，罗小丽，孙小妹，李胜富，周晖.神经元原代培养方法的改进［J］.华西医学杂志，2007, 22 (3): 566-568.
［11］Goslin K, Banker G. Rat hippocampal neurons in low density culture ［M］//Banker G, Goslin K. Culturing Nerve Cells. Cambridge, MA: MIT, 1991：252-281.
［12］Tanaka H. Culturing hippocampal neurons［J］. Nippon Yakurigaku Zasshi, 2002, 119(3): 163-166.
［13］Lesuisse C, Martin LJ. Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death［J］. J Neurobiol, 2002, 51 (1): 9-23.
［14］徐东芳，朱长太，刘东梅. 三种培养液进行海马神经元原代培养的对比研究［J］. 安徽医科大学学报，2006, 41 (5)：521-524.
［15］Brewer GJ, Torricelli JR, Evege EK, Price PJ. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination［J］. J Neurosci Res, 1993, 35(5): 567-576.