Measurement of eosinophils and interleukin-17 in nasopharyngeal secretions of children under 5 years old with wheezing

WANG Xiu-Fang; YANG Jin-Ling; QIAO Jun-Ying; ZHANG Yan-Li

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (2) : 113-116.

CLINICAL RESEARCH

Measurement of eosinophils and interleukin-17 in nasopharyngeal secretions of children under 5 years old with wheezing

WANG Xiu-Fang, YANG Jin-Ling, QIAO Jun-Ying, ZHANG Yan-Li

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Abstract

OBJECTIVE: To study the value of eosinophils (EOS) and interleukin-17 (IL-17) in nasopharyngeal secretions in the evaluation of progress of wheezing in children under 5 years old. METHODS: Fifty-three children under five years old who had recurrent wheezing were classified into two groups: wheezing group I with atopic body (n=27) and wheezing group II without atopic body (n=26). Twenty pre-surgical children with non-infectious disease were used as the control group. Nasopharyngeal secretions were collected. Inflammatory cells in nasopharyngeal secretions were counted under the microscope. IL-17 levels in supernatants were measured using ELISA. RESULTS: EOS counts in nasopharyngeal secretions in wheezing group I were significantly higher than those in wheezing group II and the control group (P<0.05, P<0.01, respectively). There were no significant differences in EOS counts between wheezing II and the control groups. The IL-17 levels in both wheezing groups were significantly higher than those in the control group (P<0.01), and the wheezing group I had increased IL-17 levels than wheezing group II (1 474±974 pg/mL vs 788±132 pg/mL; P<0.05). The IL-17 level was positively correlated with the EOS counts in wheezing group I (r=0.62, P<0.05). CONCLUSIONS: EOS counts and IL-17 levels in nasopharyngeal secretions may be used as indices for identifying the tendency to develop asthma in children under 5 years old with wheezing.［Chin J Contemp Pediatr, 2010, 12 (2):113-116］

Key words

Wheezing / Nasopharyngeal secretion / Interleukin-17 / Eosinophil / Child

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WANG Xiu-Fang, YANG Jin-Ling, QIAO Jun-Ying, ZHANG Yan-Li. Measurement of eosinophils and interleukin-17 in nasopharyngeal secretions of children under 5 years old with wheezing[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(2): 113-116

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