NMDAR expression in the cochlear nucleus and hearing damage in neonatal rats with hyperbilirubinemia

LI Ke-Fang; DU Kai-Xian; JIANG Yue-Xing; DONG Xue-Lei; ZHANG Yan

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (3) : 201-204.

EXPERIMENTAL RESEARCH

NMDAR expression in the cochlear nucleus and hearing damage in neonatal rats with hyperbilirubinemia

LI Ke-Fang, DU Kai-Xian, JIANG Yue-Xing, DONG Xue-Lei, ZHANG Yan

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Abstract

ObjectiveTo study the role of N-methyl-D-aspartate-receptor (NMDAR) expression in the development of hearing damage in neonatal rats with hyperbilirubinemia. METHODS: Sixty seven-day-old Sprague-Dawley rats were randomly injected with bilirubin of 100 μg/g (low-dose treatment group) or 200 μg/g (high-dose treatment group) or normal saline (control group). Auditory brainstem response (ABR) was examined. The concentrations of bilirubin in blood and brain were measured. NMDAR expression in the cochlear nucleus slices was examined by immunohistochemistry assay. RESULTS: ABR reflecting threshold obviously increased, and I, II and III wave latency as well as I-II, II-III and I-III interval were more prolonged in the two bilirubin treatment groups when compared with the control group. The NMDAR expression in the cochlear nucleuse in the two bilirubin treatment groups was obviously lower than that in the control group. The NMDAR expression in the cochlear nucleuse was negatively correlated with the brain bilirubin content and the ABR reflecting threshold in the two bilirubin treatment groups. CONCLUSIONS: An increased NMDAR activity may play an important role in hearing damage following hyperbilirubinemia.［Chin J Contemp Pediatr, 2010, 12 (3):201-204］

Key words

Hyperbilirubinemia / N-methyl-D-aspartate-receptor / Auditory brainstem response / Hearing damage / Neonatal rats

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LI Ke-Fang, DU Kai-Xian, JIANG Yue-Xing, DONG Xue-Lei, ZHANG Yan. NMDAR expression in the cochlear nucleus and hearing damage in neonatal rats with hyperbilirubinemia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(3): 201-204

References

［1］Rhee CK, Park HM, Jang YJ . Audiologic evaluation of neonates with severe hyperbilirubinemia using transiently evoked otoacoustic emissions and auditory brainstem responses［J］. Laryngoscope, 1999, 109(12):2005-2008.
［2］Inglefield JR, Schwartz-Bloom RD. Activation of excitatory amino acid receptors in the rat hippocampal slice increase intracellular Cl-and cell volume［J］. J Neuochem, 1998, 71(4):1396-1404.
［3］Simmons JL, Beauchaine KL. Auditory neuropathy: case study with hyperbilirubinemia［J］. J Am Acad Audiol, 2000, 11(6):337-347.
［4］陈舜年,贲晓明,夏振伟. 胆红素脑病动物模型制作与鉴定［J］. 新生儿科杂志, 1997, 12(4):166-169.
［5］Elizebeth S. Laboratory measurment of bilirubin［J］. Clin Perinatol, 1990, 17(2):397-416.
［6］Wennberg RP, Johanson BB, Folbrearova J, Siesjo BK. Bilirubin induced changes in brain energy metabolism after osmotic opening of the blood-brain barrier［J］. Pediatr Res, 1991, 30(3):473-478.
［7］田秀芬,董明敏. 豚鼠耳蜗核复合体的解剖［J］. 河南医科大学学报, 2000, 35(6):502-503.
［8］王平宇. 大白鼠中枢神经系统解剖学基础［M］.北京:人民卫生出版社,1987:9-120.
［9］第二军医大学生理学教研室. 常用实验动物脑立体定位图谱［M］.北京:科学出版社,1990:16-18.
［10］杜开先，贾天明，栾斌，马艳华，魏琛.血清胆红素浓度对足月新生儿神经心理发育的影响［J］. 中国当代儿科杂志，2008，10（3）：393-394.
［11］倪道凤,杜明,梁兴群,刘海，徐春晓，李奉荣. 不同龄豚鼠听性脑干反应和耳蜗核形态比较［J］. 听力学及言语疾病杂志, 1997, 5(3):113-117.
［12］Grojean S, Koziel V, Vert P, Daval JL. Billirubin induces apoptosis via activation of NMDA receptors in developing rat brain neurons［J］. Exp Neurol, 2000, 166(2): 334-341.