Effects of levetiracetam on the expression of NCAM and GAP-43 mRNA in the hippocampus of rats with epilepsy

JIA Tian-Ming; LIU Tao; LUAN Bin; LI Xiao-Li; ZHANG Xiao-Li

PDF(1069 KB)

Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (5) : 428-431.

EXPERIMENTAL RESEARCH

Effects of levetiracetam on the expression of NCAM and GAP-43 mRNA in the hippocampus of rats with epilepsy

JIA Tian-Ming, LIU Tao, LUAN Bin, LI Xiao-Li, ZHANG Xiao-Li

Author information +

History +

Abstract

OBJECTIVE: This study explored the effects of levetiracetam (LEV) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP-43) mRNA in the hippocampus of rats with epilepsy induced by lithium-pilocarpine (Li-PILO) in order to provide a basis for investigating the antiepileptic mechanism of LEV and its doseresponse. METHODS: Forty-eight Wistar rats were randomly divided into a normal control, a Li-PILO model and two LEV treatment groups (LEV: 150 and 300 mg/kg) (n=12 each). The LEV treatment groups received LEV by intragastric administration 6 hrs after status epilepticus (once daily for 2 two weeks). The expressions of NCAM and GAP-43 mRNA in the hippocampus was determined by real-time PCR. RESULTS: The expression of NCAM and GAP-43 mRNA in the Li-PILO model group was significantly higher than in the normal control group (P<0.05). LEV treatment of 150 and 300 mg/kg significantly decreased the expression of NCAM and GAP-43 mRNA compared with the Li-PILO model group (P<0.05). The LEV treatment group at the dose of 300 mg/kg showed significantly lower expression of NCAM and GAP-43 mRNA than the 150 mg/kg LEV treatment group (P<0.05). CONCLUSIONS: Li-PILO can up-regulate the expressions of NCAM and GAP-43 mRNA in the hippocampus of rats with epilepsy. LEV can inhibit the expression of NCAM and GAP-43 mRNA and the effect is associated with the dose of LEV.

Key words

Epilepsy / Levetiracetam / Nerve cell adhesion molecule / Growth-associated protein 43 / Rats

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

JIA Tian-Ming, LIU Tao, LUAN Bin, LI Xiao-Li, ZHANG Xiao-Li. Effects of levetiracetam on the expression of NCAM and GAP-43 mRNA in the hippocampus of rats with epilepsy[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(5): 428-431

References

［1］Sato K, Iwai M, Nagano I, Shoji M, Abe K. Temporal and spacial changes of highly polysialylated neural cell adhesion molecule immunoreactivity in amygdala kindling development［J］. Neurolcal Res, 2003, 25 (1): 79-82.

［2］Longo B, Vezzani A, Mello LE. Growth-associated protein 43 expression in hippocampal molecular layer of chronic epileptic rats treated with cycloheximide［J］.Epilepsia, 2005, 46(Suppl 5):125-128.

［3］高志杰，姜玉武. 左乙拉西坦单药治疗小儿癫癎的疗效和安全性随访研究［J］.中国当代儿科杂志，2008,10（6）：711-714.

［4］Bootsma HP, Ricker L, Diepman L, Gehring J, Hulsman J, Lambrechts D, et al. Levetiracetam in clinical practice: long-term experience in patients with refractory epilepsy referred to a tertiary epilepsy center［J］. Epilepsy Behav, 2007, 10 (2): 296-303.

［5］De Smedt T, Raedt R, Vonck K, Boon P. Levetiracetam: part II, the clinical profile of a novel anticonvulsant drug［J］. CNS Drug Rev,2007,13 (1): 57-78.

［6］De Smedt T, Raedt R, Vonck K, Boon P.Levetiracetam: the profile of a novel anticonvulsant drugpart I:preclinical data［J］. CNS Drug Rev, 2007, 13(l): 43-56.

［7］Racine RJ, Steingart M, Mclntyre DC. Development of kindling-prone and kindling-resistant rats:seleetive breeding and electrophysiological studies［J］. Epilepsy Res, 1999, 35(3): 183-195.

［8］Muller CJ, Bankstahl M, Groticke I, Loscher W. Pilocarpine vs. lithium-pilocarpine for induction of status epilepticus in mice: development of spontaneous seizures, beha-vioral aterations and neuronal damage［J］. Eur J Pharmacar, 2009, 619(1-3): 15-24.

［9］程敏，黄志，李思秀.左乙拉西坦预防动物热性惊厥的实验研究［J］.儿科药学杂志，2009，15(4)：16-18.

［10］Racine RJ.Modification of seizure activity by electrical stimulation.Ⅱ.Motor seizure［J］. Electroencephalogr Clin Neurophysiol, 1972, 32(3): 281-294.

［11］Leite JP, Neder L, Arisi GM, Carlotti CG Jr, Assirati JA, Moreira JE. Plasticity, synaptic strength, and epilepsy: what can we learn from ultrastructural data?［J］. Epilepsia, 2005, 46 (Suppl 5): 134-141.

［12］Cavazos JE, Jones SM, Cross DJ. Sprouting and synaptic reorganization in the subiculum and CA1 region of the hippocampus in acute and chronic models of partialonset epilepsy［J］. Neuroscience, 2004, 126 (3): 677-688.

［13］徐慧琴，朱遂强，郑荣远，王开颜，李安乐，杨学志.托吡酯对戊四氮点燃慢性癫癎大鼠海马神经细胞黏附分子的影响［J］. 中国临床神经科学，2006，14(5):491-493.

［14］Lynch BA, Lambeng N, Nocka K, Kensel-Hammes P, Bajjalieh SM, Matagne A, et al. The synaptic vesicle protein SV2A is the binding site for the antiepileptic drug levetiracetam［J］. Proc Natl Acad Sci U S A, 2004, 101 (26): 9861-9866.

［15］王学禹，李明霞.左乙拉西坦在癫癎治疗中的应用进展［J］.山东医药，2008，48（11）：110-111.