OBJECTIVE: To explore the causes of nonspecific chronic cough in children and relationship between transient receptor potential vanilloid 1 (TRPV1) gene polymorphisms and nonspecific chronic cough. METHODS: A total of 195 children with chronic cough were followed up half a month, one month and three months after their first visit to hospital. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine polymorphisms of the TRPV1 gene in the children. A total of 205 healthy or surgical children without chronic cough served as the control group. RESULTS: The etiologic distribution of the 195 children with chronic cough was as follows: 96 (49.2%) cases of cough variant asthma (CVA), 48 (24.6%) cases of CVA complicated by upper airway cough syndrome (UACS), 34 (17.4%) cases of post-infectious cough, and 17 (8.7%) cases of UACS. Three genotypes were identified in both groups at positions rs222747 (CC, GC and GG), rs222748 (CC, TC and TT) and rs8065080 (CC, TC and TT). The frequencies of genotype and allele at position rs222747 did not accord with the law of Hardy-Weinberg. There was no significant difference in frequencies of genotype and allele at positions rs222748 and rs8065080 between the two groups. CONCLUSIONS: CVA, UACS and post-infectious cough are common causes of nonspecific chronic cough in children. TRPV1 gene polymorphisms at positions rs222748 and rs8065080 may be unrelated to nonspecific chronic cough in children.
Key words
Chronic cough /
Transient receptor potential vanilloid 1 /
Gene polymorphism /
Child
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
［1］中华医学会呼吸病学分会哮喘学组. 咳嗽的诊断与治疗指南（草案）［J］.中华结核和呼吸杂志，2005，28（11）：738-744.
［2］Leonardi GS, Houthuijs D, Nikiforov B, Volf J, Rudnai P, Zejda J, et al. Respiratory symptoms, bronchitis and asthma in children of Central and Eastern Europe［J］. Eur Respir J, 2002, 20(4): 890-898.
［3］Lee LY, Ni D, Hayes D Jr, Lin RL. TRPV1 as a cough sensor and its temperature-sensitive properties［J］. Pulm Pharmacol Ther, 2011, 24(3): 280-285.
［4］王强，白雪涛，徐东刚，徐东群，李红，方建龙，等. TRPV1 UTR-3基因多态性与北京汉族儿童哮喘的易感性研究［J］. 卫生研究，2009，38（5）：516-521.
［5］中华医学会儿科学分会呼吸学组《中华儿科杂志》编辑委员会. 儿童慢性咳嗽诊断与治疗指南(试行)［J］. 中华儿科杂志，2008，46（2）：104-107.
［6］Cantero-Recasens G, Gonzalez JR, Fandos C, Duran-Tauleria E, Smit LA, Kauffmann F, et al. Loss of function of transient receptor potential vanilloid 1(TRPV1) genetic variant is associated with lower risk of active childhood asthma［J］. J Biol Chem, 2010, 285(36): 27532-27535.
［7］张晓波，农光民.儿童慢性咳嗽111例病因分析［J］.中国当代儿科杂志，2011，13（2）：131-134.
［8］Lee LY, Pisarri T. Afferent properties and reflex functions of bronchopulmonary C-fibers［J］. Respir Physiol, 2001, 125(1-2): 47-65.
［9］Mitchell JE, Campbell AP, New NE, Sadofsky LR, Kastelik JA, Mulrennan SA, et al. Expression and characterisation of the intracellular vanilloid receptor (TRPV1) in bronchi from patients with chronic cough［J］. Exp Lung Res, 2005, 31(3): 295-306.
［10］Groneberg DA, Niimi A, Dinh QT, Cosio B, Hew M, Fischer A, et al. Increased expression of transient receptor potential vallinoid-1 in airway nerves of chronic cough［J］. Am J Respir Crit Care Med, 2004, 170(12):1276-1280.
［11］Veronesi B, Oortgiesen M. The TRPV1 receptor: target of toxicants and therapeutics［J］. Toxicol Sci, 2006, 89(1):1-3.
PDF(1236 KB)
