Role of miRNA-126/miRNA-126* in the fetal lung development of rats

YANG Yang; KAN Qing; QIU Jie; PU Xiao-Dan; ZHANG Pan; ZHANG Xiao-Qun; ZHOU Xiao-Yu

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Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (09) : 708-712.

EXPERIMENTAL RESEARCH

Role of miRNA-126/miRNA-126* in the fetal lung development of rats

YANG Yang, KAN Qing, QIU Jie, PU Xiao-Dan, ZHANG Pan, ZHANG Xiao-Qun, ZHOU Xiao-Yu

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Abstract

OBJECTIVE: To investigate the expression and role of miRNA-126/miRNA-126* in the fetal lung development of rats. METHODS: Twelve pregnant Sprague-Dawley rats were randomly divided into 3 groups and the fetal rats were removed at 16, 19 and 21 days of gestation respectively. Hematoxylin and eosin staining was performed to observe lung morphology of fetal rats. Then microRNA (miRNA) microarray was used to study the expression patterns of miRNA-126/miRNA-126* in fetal lungs at the three time points. And miRNA-126* was selected for further study by real-time PCR. RESULTS: There was no evident difference in the expression of miRNA-126 among the three groups, however the expression level of miRNA-126* increased gradually as the fetal lung developed. The real-time PCR result further showed that expression of miRNA-126* increased gradually with lung development, displaying significant differences among the three groups (P<0.05). CONCLUSIONS: miRNA-126* may play an important role in development of the fetal lung in rats.

Key words

Lung development / miRNA-126* / miRNA-126 / Rats

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YANG Yang, KAN Qing, QIU Jie, PU Xiao-Dan, ZHANG Pan, ZHANG Xiao-Qun, ZHOU Xiao-Yu. Role of miRNA-126/miRNA-126* in the fetal lung development of rats[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(09): 708-712

References

［1］Croce CM, Calin GA. miRNAs, cancer, and stem cell division［J］. Cell, 2005, 122(1): 6-7.

［2］Iorio MV, Croce CM. MicroRNAs in cancer: small molecules with a huge impact［J］. J Clin Oncol, 2009, 27(34): 5848-5856.

［3］Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14［J］.Cell, 1993, 75(5): 843-854.

［4］Grimson A, Farh KK, Johnston WK, Garrett-Engele P, Lim LP, Bartel DP. MicroRNA targeting specificity in mammals: determinants beyond seed pairing［J］. Mol Cell, 2007, 27(1): 91-105.

［5］Stefani G, Slack FJ. Small non-coding RNAs in animal development［J］. Nat Rev Mol Cell Biol, 2008, 9(3): 219-230.

［6］Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, et al. miR-126 regulates angiogenic signaling and vascular integrity［J］. Dev Cell, 2008, 15(2): 272-284.

［7］Burri PH. Fetal and postnatal development of the lung［J］. Annu Rev Physiol, 1984, 46: 617-628.

［8］Zoetis T, Hurtt ME. Species comparison of lung development［J］. Birth Defects Res B Dev Reprod Toxicol, 2003, 68 (2): 121-124.

［9］Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T. Identification of tissue specific microRNAs from mouse［J］. Curr Biol, 2002, 12(9): 735-739.

［10］Wang S, Aurora AB, Johnson BA, Qi X, McAnally J, Hill JA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis［J］. Dev Cell, 2008, 15(2): 261-271. 14

［11］Liu B, Peng XC, Zheng XL, Wang J, Qin YW. MiR-126 restoration down-regulate VEGF and inhibit the growth of lung cancer cell lines in vitro and in vivo［J］. Lung Cancer, 2009, 66(2): 169-175.

［12］Liu B, Peng XC, Zheng XL, Wang J, Qin YW. MiR-126 restoration down-regulate VEGF and inhibit the growth of lung cancer cell lines in vitro and in vivo［J］. Lung Cancer, 2009, 66(2): 169-175.

［13］Crawford M, Brawner E, Batte K, Yu L, Hunter MG, Otterson GA, et al. MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines［J］. Biochem Biophys Res Commun, 2008, 373(4): 607-612.

［14］Yang Y, Li X, Yang Q, Wang X, Zhou Y, Jiang T, et al. The role of microRNA in human lung squamous cell carcinoma［J］. Cancer Genet Cytogenet, 2010, 200(2): 127-133.

［15］Musiyenko A, Bitko V, Barik S. Ectopic expression of miR-126*, an intronic product of the vascular endothelial EGF-like 7 gene, regulates prostein translation and invasiveness of prostate cancer LNCaP cells［J］. J Mol Med (Berl), 2008, 86(3): 313-322.

［16］Naora H, Deacon NJ. Relationship between the total size of exons and introns in protein-coding genes of higher eukaryotes［J］. Proc Natl Acad Sci USA, 1982, 79(20): 6196-6200.

［17］Clement JQ, Qian L, Kaplinsky N, Wilkinson MF. The stability and fate of a spliced intron from vertebrate cells［J］. RNA, 1999, 5(2): 206-220.

［18］Parker LH, Schmidt M, Jin SW, Gray AM, Beis D, Pham T, et al. The endothelial-cell-derived secreted factor Egfl7 regulates vascular tube formation［J］. Nature, 2004, 428(6984): 754-758.

［19］Fitch MJ, Campagnolo L, Kuhnert F, Stuhlmann H. EGFL7, a novel epidermal growth factordomain gene expressed in endothelial cells［J］. Dev Dyn, 2004, 230(2): 316-324.

［20］Campagnolo L, Leahy A, Chitnis S, Koschnick S, Fitch MJ, Fallon JT, et al. EGFL7 is a chemoattractant for endothelial cells and is up-regulated in angiogenesis and arterial injury［J］. Am J Pathol, 2005, 167(1): 275-284.

［21］Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function［J］. Cell, 2004, 116(2): 281-297.

［22］Saito Y, Friedman JM, Chihara Y, Egger G, Chuang JC, Liang G. Epigenetic therapy upregulates the tumor suppressor microRNA-126 and its host gene EGFL7 in human cancer cells［J］. Biochem Biophys Res Commun, 2009, 379(3): 726-731.

［23］Ro S, Park C, Young D, Sanders KM, Yan W. Tissue-dependent paired expression of miRNAs［J］. Nucleic Acids Res, 2007, 35(17):5944-5953.

［24］Okamura K, Phillips MD, Tyler DM, Duan H, Chou YT, Lai EC. The regulatory activity of microRNA* species has substantial influence on microRNA and 3′UTR evolution［J］. Nat Struct Mol Biol, 2008, 15(4): 354-363.

［25］Meister J, Schmidt MH. miR-126 and miR-126*: new players in cancer［J］. Scientific World Journal, 2010, 10: 2090-2100.

［26］Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, et al. miR-126 regulates angiogenic signaling and vascular integrity［J］. Dev Cell, 2008, 15(2): 272-284.

［27］Li X, Shen Y, Ichikawa H, Antes T, Goldberg GS. Regulation of miRNA expression by Src and contact normalization: effects on nonanchored cell growth and migration［J］. Oncogene, 2009, 28(48): 4272-4283.

［28］陈幽，韩玉昆，叶贞治，卢光进. 呼吸窘迫综合征并发支气管肺发育不良危险因素分析［J］. 中国当代儿科杂志，2007，9(1)：15-18.