OBJECTIVE: Suppressors of cytokine signaling (SOCS) have been shown to play an important role in regulating cytokines, such as intracellular interferon (IFN) and interleukin (IL), in the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway. At present, the association between SOCS and asthma is still under study. The aim of this study is to explore the relationship of SOCS-1 and SOCS-3 mRNA expression in peripheral blood mononuclear cells (PBMCs) with the intracellular IFN-γ/IL-4 ratio in CD4+ T cells and specific IgE (sIgE) level in children with asthma. METHODS: BMCs were collected from 44 children with allergic asthma (4-14 years) and 30 healthy children. The intracellular IFN-γ/IL-4 ratio in CD4+ T cells was measured by flow cytometry. Total RNAs were extracted from the PBMCs and SOCS-1 and SOCS-3 mRNA expression was measured by SYBR Green I quantitative RT-PCR. RESULTS: Compared with the healthy children, children with allergic asthma showed a lower level of intracellular IFN-γ in peripheral blood ［(15.7 ± 2.0)% vs (19.1 ± 2.7)%］ and IFN-γ/IL-4 ratio (3.4 ± 1.5 vs 4.8 ± 2.9) and higher SOCS-1 mRNA expression (-Ct, 11.1 ± 1.9 vs 12.6 ± 2.8）.There was a negative relationship between SOCS-1 mRNA expression and the percentage of IFN-γ-producing CD4+ T cells in peripheral blood in both asthmatic and healthy children (P<0.05). No correlation was found between SOCS-1 and SOCS-3 expression and sIgE level. CONCLUSIONS: Children with allergic asthma have elevated levels of SOCS-1 mRNA in PBMCs, which is associated with Th2-skewed immune response.
Key words
Asthma /
Suppressors of cytokine signaling /
Th1/Th2 /
Child
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
［1］阳艳丽，潘玉琴，何帮顺，钟天鹰. 哮喘患儿外周血调节性T细胞和Th1/Th2的变化及其与哮喘病情的关系［J］. 中国当代儿科杂志，2011，13（6）：482-486.
［2］董蔚，盛军，浦燕艳，石学耕，史桂英. 哮喘患儿细胞内IL-4 IFN-γ和血清IgE测定及临床意义［J］. 中国当代儿科杂志，2002， 4（2）：81-82.
［3］Knisz J, Rothman PB. Suppressor of cytokine signaling in allergic Inflammation［J］. J Allergy Clin Immunol, 2007, 119(3): 739-745.
［4］Egwuagu CE, Yu CR, Zhang M, Mahdi RM, Kim SJ, Gery I. Suppressors of cytokine signaling proteins are differentially expressed in Th1 and Th2 cells: implications for Th cell lineage commitment and maintenance［J］. J Immunol, 2002,168(7): 3181-3187.
［5］中华医学会儿科学分会呼吸学组，中华医学会《中华儿科杂志》编辑委员会. 儿童支气管哮喘防治常规（试行）［J］.中华儿科杂志,2004,42(2):100-106.
［6］中华医学会耳鼻咽喉科学分会，中华耳鼻咽喉科杂志编辑委员会，变应性鼻炎诊断标准及疗效评定标准［J］. 中华儿鼻咽喉科杂志, 1998,33(3):134-135.
［7］Daegelmann C, Herberth G, Roder S, Herbarth O, Giese T, Krmer U, et al. Association between suppressors of cytokine signalling, T-helper type 1/T-helper type 2 balance and allergic sensitization in children［J］. Clin Exp Allergy, 2008, 38(3): 438-448.
［8］Yoshimura A, Naka T, Kubo M. SOCS proteins, cytokine signalling and immune regulation［J］. Nat Rev Immunol, 2007, 7(6): 454-465.
［9］Cassel SL, Rothman PB. Chapter 3: Role of SOCS in allergic and innate immune responses［J］. Adv Immunol, 2009, 103: 49-76.
［10］Fukuyama S, Nakano T, Matsumoto T, Oliver BG, Burgess JK, Moriwaki A, et al. Pulmonary suppressor of cytokine signaling-1 induced by IL-13 regulates allergic asthma phenotype［J］. Am J Respir Crit Care Med, 2009, 179(11): 992-998.
［11］Seki Y, Inoue H, Nagata N, Hayashi K, Fukuyama S, Matsumoto K, et al. SOCS-3 regulates onset and maintenance of T(H)2-mediated allergic responses［J］. Nat Med, 2003, 9(8): 1047-1054.
［12］李广付，杨桂枝，阎冰，王芳，李玉琴. 细胞因子信号转导抑制因子3和5在儿童过敏性哮喘特异性免疫治疗中变化的研究［J］. 中国实用儿科杂志，2008,23(4):257-259.
PDF(919 KB)
