Effects of bone marrow mesenchymal stem cell transplantation on retinal cell apoptosis in premature rats with retinopathy

ZHAO Yan-Song; ZHAO Kan-Xing; WANG Xiao-Li; CHEN Yu-Xi; WANG Li; MU Qing-Jie

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Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (12) : 971-975.

Effects of bone marrow mesenchymal stem cell transplantation on retinal cell apoptosis in premature rats with retinopathy

ZHAO Yan-Song, ZHAO Kan-Xing, WANG Xiao-Li, CHEN Yu-Xi, WANG Li, MU Qing-Jie

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Abstract

OBJECTIVE: To explore the effects of marrow mesenchymal stem cell (BMSC) transplantation on retinal cells apoptosis and changes to neurotrophin-3 (NT-3 and ciliary neurotrophic factor (CNTF) in rats with retinopathy of prematurity (ROP). METHODS: Seven-day-old Sprague-Dawley rats were randomly divided into normal control (CON), ROP, BMSC transplantation (BMSCs were transplanted 5 days after oxygen conditioning) and phosphate buffered saline (PBS) groups. The ROP model was prepared according to the classic hyperoxygen method. Seven days after transplantation, TUNEL/DAPI, NT-3/API and CNTF/DAPI double-labeled immunofluorescence were used to examine the effects of BMSC transplantation on both the apoptosis of retinal cells and the expression of NT-3 and CNTF protein in the retinal cells of the ROP rats. RESULTS: Seven days after BMSC transplantation, there were few TUNEL+ DAPI+ cells observed in the CON group. There were fewer TUNEL+DAPI+ cells observed in the BMSC group than in the ROP group (P0.05). There were few NT-3+DAPI+ cells and CNTF+DAPI+ cells in the CON group. There were more NT-3+DAPI+ and CNTF+DAPI+ cells in the ROP group than in the CON group, but there was no significant difference between the ROP and CON groups (P>0.05). More NT-3+DAPI+ and CNTF+DAPI+ cells were observed in the BMSC group compared with the ROP group (P0.05). CONCLUSIONS: BMSC transplantation therapy could alleviate the apoptosis of retinal cells in ROP rats, and its mechanisms might be associated with promoting the expression of NT-3 and CNTF protein in retinal cells.

Key words

Retinopathy / Bone marrow mesenchymal stem cell transplantation / Apoptosis / Neurotrophin-3 / Ciliary neurotrophic factor / Rats

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ZHAO Yan-Song, ZHAO Kan-Xing, WANG Xiao-Li, CHEN Yu-Xi, WANG Li, MU Qing-Jie. Effects of bone marrow mesenchymal stem cell transplantation on retinal cell apoptosis in premature rats with retinopathy[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(12): 971-975

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