OBJECTIVE: To study the effects of environmental enrichment on neuron proliferation, learning and memory ability and motor ability in neonatal rats with hypoxic-ischemic brain damage (HIBD). METHODS: One hundred and eight 7-day-old Sprague-Dawley rats were randomly divided into three groups: sham operation (CON group), HIBD and intervention group. HIBD model was prepared according to the classic Rice-Vannucci method. Environmental enrichment was administered for the rats in the intervention group after HIBD inducement. Behavioral tests (Water maze test, Suspension test and Slope test) were performed and the number of neural cells in the left hippocampus was examined 7, 14 and 28 days after intervention. RESULTS: The pyramid cells in the hippocampus CA1 area in the HIBD group were significantly less than in the CON group at 7, 14 and 28 days (P<0.05). The number of pyramid cells in the hippocampus CA1 area in the intervention group was significantly higher than in the HIBD group (P<0.01) at 7, 14 and 28 days. The hidden platform escape latency period (EL) in the Water maze test was significantly more prolonged and the cross-platform number within 2 minutes was significantly less in the HIBD and the intervention groups than in the CON group at all observed time points (P<0.01). The EL was significantly shorter and the cross-platform number within 2 minutes was significantly higher in the intervention group than in the HIBD group at all observed time points (P<0.01). The maintain time and score in the Suspension test were significantly lower and the time in the Slope test was significantly more prolonged in the HIBD and intervention groups than in the CON group at 7, 14 and 28 days (P<0.01). An increased maintain time and score and a decreased time in the Slope test were found in the intervention group compared with the HIBD group at 14 and 28 days (P<0.01). CONCLUSIONS: Environmental enrichment can improve motor function, learning and memory ability, and promote the repair and proliferation of neurons in neonatal rats with HIBD.
Key words
Hypoxic-ischemic brain damage /
Environmental enrichment /
Neuron /
Behavioral test /
Rats
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References
［1］陈光福，张蕴芳，龙琦，温文，许美权. 早期干预对缺氧缺血性脑损伤新生大鼠内源性神经干细胞增殖的影响［J］. 实用儿科临床杂志，2011,26(20):1575-1577.
［2］Rice JE, Vannucci RC, Brierley JB. The influence of immaturity on hypoxic-ischemic brain damage in the rat［J］. Ann Neural, 1981,9(2):131-141.
［3］Couto RC, Carvalho EA, Pedrosa TM, Pedroso ER, Neto MC, Biscione FM. A 10-year prospective surveillance of nosocomial infections in neonatal intensive care units［J］. Am J Infect Control, 2007, 35(3): 183-189.
［4］陈燕惠，刘玲，陈敏榕，陈达光. 早期干预对新生鼠脑神经生长相关蛋白表达及细胞凋亡的影响［J］. 实用儿科临床杂志,2007, 22 (14):1083-1084.
［5］马良，陈燕惠，韦立新. 早期丰富环境对大鼠远期行为发育及血清皮质酮的影响［J］. 中国当代儿科杂志,2011,13 (7): 586-589.
［6］沈晓明.儿童脑科学研究的临床和教育学意义:从研究到应用［J］.中国儿童保健杂志,2006,14(1):1.
［7］胡金梅,何展文,刘木金, 罗向阳.早期综合干预对脑损伤综合征患儿的疗效［J］.中国康复杂志,2009,24(2):140.
［8］胡素君,蔡春玲,王玲.早期干预对高危新生儿智能及运动发育的影响［J］.中国实用神经疾病杂志,2008,11(2):86-88.
［9］Liu YP, Bradley TL, Mustafa KB, Dempsey RJ, Vemuganti R. The potential of neural stem cells to repair stroke-induced brain damage［J］. Acta Neuropathol, 2009,117（5）:469-480.
［10］Rueger MA, Backes H, Walberer M, Neumaier B, Ullrich R, Simard ML, et al. Noninvasive imaging of endogenous neural stem cell mobilization in vivo using positron emission tomography［J］. J Neurosci, 2010, 30(18): 6454-6460.
［11］Nithianantharajah J, Hannan AJ. Enriched environments, experience-dependent plasticity and disorders of the nervous system［J］. N Rev Neurosci, 2006, 7(9): 697.
［12］Maegele M, Lippert-Gruener M, Ester-Bode T, Garbe J,Bouillon B,Neugebauer E. et al. Multimodal early onset stimulation combined with enriched environment is associated with reduced CNS lesion volume and enhanced reversal of neuromotor dysfunction after traumatic brain injury in rats［J］. Eur J Neurosci, 2005, 21(9): 2406-2418.
［13］熊方令, 高宜录. 内源性神经干细胞修复中枢神经系统损伤的策略［J］. 基础医学与临床, 2010,30(10):1105-1108.
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