2017~2019年儿童诺如病毒相关性胃肠炎的分子流行病学研究

doi:10.7499/j.issn.1008-8830.2107003

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Abstract Objective To study the molecular epidemiological characteristics of norovirus in children with acute gastroenteritis from 2017 to 2019. Methods A retrospective analysis was performed on the medical data of children with acute gastroenteritis who were admitted to Children's Hospital of Chongqing Medical University from January 2017 to December 2019. A total of 1 458 stool samples were collected from the children, and viral RNA was extracted. Reverse transcription polymerase chain reaction was used for gene amplification, sequencing, and genotype identification of the VP1 region of capsid protein in norovirus. Results Among the 1 458 stool samples, 158 (10.8%) were positive for norovirus. There was no significant difference in the positive detection rate of norovirus between different years (P>0.05). Boys had a norovirus detection rate of 12.2% (105/860), which was significantly higher than that in girls (8.9%, 53/598) (P=0.043). The children aged 12 to <18 months had the highest norovirus detection rate (16.9%, 51/301). August, September, and October were the epidemic peak season. A total of 23 norovirus-positive samples were also positive for rotavirus. The norovirus detected were mainly GII type (97.5%, 154/158), and only 4 cases were GI type (2.5%, 4/158). The sequencing of the VP1 region of capsid protein in the positive samples showed that GII.4 (69.6%, 110/158) was the dominant genotype, among which 99 (62.7%, 99/158) were GII.4 Sydney 2012, followed by GII.3 (15.2%, 24/158), GII.2 (10.1%, 16/158), GII.6 (1.9%, 3/158), and GII.17 (0.6%, 1/158). GI.3 (1.3%, 2/158), GI.2 (0.6%, 1/158), and GI.5 (0.6%, 1/158) were rarely detected. Conclusions Norovirus GII.4 Sydney 2012 was the major epidemic strain in the children with norovirus gastroenteritis from 2017 to 2019. Although norovirus infection can exist throughout the year, August to October is the peak period. During this period, norovirus surveillance and key population protection are strengthened to help prevent and control norovirus diarrhea.

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	Articles by authors
	DUAN Mei-Lin
	HU Yue
	TANG Xiang
	XU Hong-Mei

Key words： Acute gastroenteritis Norovirus Epidemiology Genotype Child

Received: 02 July 2021

Cite this article:

DUAN Mei-Lin,HU Yue,TANG Xiang et al. A molecular epidemiological study of pediatric norovirus gastroenteritis, 2017-2019[J]. CJCP, 2021, 23(10): 1027-1032.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2107003 OR http://www.zgddek.com/EN/Y2021/V23/I10/1027

	Bányai K, Estes MK, Martella V, et al. Viral gastroenteritis[J]. Lancet, 2018, 392(10142): 175-186. PMID: 30025810. DOI: 10.1016/S0140-6736(18)31128-0.
	Chen YFE, Wang CY, Chiu CH, et al. Molecular epidemiology and clinical characteristics of norovirus gastroenteritis with seizures in children in Taiwan, 2006-2015[J]. Medicine (Baltimore), 2019, 98(40): e17269. PMID: 31577718. PMCID: PMC6783164. DOI: 10.1097/MD.0000000000017269.
	Shah MP, Hall AJ. Norovirus illnesses in children and adolescents[J]. Infect Dis Clin North Am, 2018, 32(1): 103-118. PMID: 29406972. PMCID: PMC6814392. DOI: 10.1016/j.idc.2017.11.004.
	Bartsch SM, Lopman BA, Ozawa S, et al. Global economic burden of norovirus gastroenteritis[J]. PLoS One, 2016, 11(4): e0151219. PMID: 27115736. PMCID: PMC4846012. DOI: 10.1371/journal.pone.0151219.
	沈玲羽, 姜博, 严寒秋, 等. 诺如病毒基因分型研究进展[J]. 中华流行病学杂志, 2020, 41(11): 1927-1932. PMID: 33297663. DOI: 10.3760/cma.j.cn112338-20200302-00234.
	Chhabra P, de Graaf M, Parra GI, et al. Updated classification of norovirus genogroups and genotypes[J]. J Gen Virol, 2019, 100(10): 1393-1406. PMID: 31483239. PMCID: PMC7011714. DOI: 10.1099/jgv.0.001318.
	7 中华人民共和国卫生部. 中国腹泻病诊断治疗方案[J]. 临床儿科杂志, 1994, 12(3): 148-151.
	Vinjé J, Hamidjaja RA, Sobsey MD. Development and application of a capsid VP1 (region D) based reverse transcription PCR assay for genotyping of genogroup I and II noroviruses[J]. J Virol Methods, 2004, 116(2): 109-117. PMID: 14738976. DOI: 10.1016/j.jviromet.2003.11.001.
	Cho SR, Chae SJ, Jung S, et al. Trends in acute viral gastroenteritis among children aged ≤5 years through the national surveillance system in South Korea, 2013-2019[J]. J Med Virol, 2021, 93(8): 4875-4882. PMID: 33219526. PMCID: PMC8360024. DOI: 10.1002/jmv.26685.
	Devant JM, Hansman GS. Structural heterogeneity of a human norovirus vaccine candidate[J]. Virology, 2021, 553: 23-34. PMID: 33202318. DOI: 10.1016/j.virol.2020.10.005.
	11 乔英琴, 余凤, 陈婉冰, 等. 2014年重庆地区511例急性腹泻儿童中诺如病毒腹泻流行特点与分子亚型鉴定[J]. 第三军医大学学报, 2016, 38(1): 101-106. DOI: 10.16016/j.1000-5404.201504160.
	12 唐香, 陈茹娟, 赖方方, 等. 2012年重庆单中心腹泻患儿感染诺如病毒及其基因型别和重组的研究[J]. 中国循证儿科杂志, 2014, 9(3): 167-171. DOI: 10.3969/j.issn.1673-5501.2014.03.002.
	13 赵进. 重庆地区门诊儿童急性腹泻相关病毒的流行特征与基因特征研究[D]. 北京: 中国人民解放军军事医学科学院, 2014.
	14 陈岑. 2015年重庆地区儿童诺如病毒性胃肠炎分子流行病学研究[D]. 重庆: 重庆医科大学, 2016.
	15 曹亿会, 杨景晖, 姜黎黎, 等. 2017-2019年我国西南地区病毒性腹泻病原检测分析[J]. 公共卫生与预防医学, 2021, 32(1): 10-13. DOI: 10.3969/j.issn.1006-2483.2021.01.003.
	李洁, 杨扬, 梁志超, 等. 北京地区五岁以下儿童病毒性急性胃肠炎病原构成与临床特征分析[J]. 中华预防医学杂志, 2020, 54(10): 1104-1110. PMID: 33115197. DOI: 10.3760/cma.j.cn112150-20191129-00892.
	17 石鑫, 舒畅, 许军, 等. 黑龙江省2017-2019年腹泻患者诺如病毒检测及基因特征分析[J]. 中国公共卫生管理, 2020, 36(4): 457-459, 498. DOI: 10.19568/j.cnki.23-1318.2020.04.005.
	Ansari SA, Springthorpe VS, Sattar SA. Survival and vehicular spread of human rotaviruses: possible relation to seasonality of outbreaks[J]. Rev Infect Dis, 1991, 13(3): 448-461. PMID: 1866549. DOI: 10.1093/clinids/13.3.448.
	Rohayem J. Norovirus seasonality and the potential impact of climate change[J]. Clin Microbiol Infect, 2009, 15(6): 524-527. PMID: 19604277. DOI: 10.1111/j.1469-0691.2009.02846.x.
	Nordgren J, Svensson L. Genetic susceptibility to human norovirus infection: an update[J]. Viruses, 2019, 11(3): 226. PMID: 30845670. PMCID: PMC6466115. DOI: 10.3390/v11030226.
	21 李静, 张婷, 邹文菁, 等. 2018-2019年湖北省5岁及以下腹泻儿童诺如病毒感染特征和病原学分析[J]. 疾病监测, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006.
	Ahmed SM, Lopman BA, Levy K. A systematic review and meta-analysis of the global seasonality of norovirus[J]. PLoS One, 2013, 8(10):e75922. PMID: 24098406. PMCID: PMC3788804. DOI: 10.1371/journal.pone.0075922.
	Farahmand M, Moghoofei M, Dorost A, et al. Global prevalence and genotype distribution of norovirus infection in children with gastroenteritis: a meta-analysis on 6 years of research from 2015 to 2020[J]. Rev Med Virol, 2021, e2237. PMID: 33793023. DOI: 10.1002/rmv.2237. Epub ahead of print.
	Zhou HL, Wang SM, von Seidlein L, et al. The epidemiology of norovirus gastroenteritis in China: disease burden and distribution of genotypes[J]. Front Med, 2020, 14(1): 1-7. PMID: 31823287. PMCID: PMC8320309. DOI: 10.1007/s11684-019-0733-5.
	van Beek J, de Graaf M, Al-Hello H, et al. Molecular surveillance of norovirus, 2005-16: an epidemiological analysis of data collected from the NoroNet network[J]. Lancet Infect Dis, 2018, 18(5): 545-553. PMID: 29396001. DOI: 10.1016/S1473-3099(18)30059-8.
	26 何菲, 方玉莲, 林书祥, 等. 2018年天津市儿童GⅡ型诺如病毒感染的分子流行病学分析[J]. 中华检验医学杂志, 2020, 43(5): 570-575. DOI: 10.3760/cma.j.cn114452-20190820-00490.
	Iritani N, Yamamoto SP, Abe N, et al. GII.17 norovirus infections in outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan during two decades[J]. J Med Virol, 2019, 91(12): 2101-2107. PMID: 31368535. DOI: 10.1002/jmv.25560.
	Lu J, Sun LM, Fang L, et al. Gastroenteritis outbreaks caused by norovirus GII.17, Guangdong Province, China, 2014-2015[J]. Emerg Infect Dis, 2015, 21(7): 1240-1242. PMID: 26080037. PMCID: PMC4480401. DOI: 10.3201/eid2107.150226.
	Mans J. Norovirus infections and disease in lower-middle- and low-income countries, 1997-2018[J]. Viruses, 2019, 11(4): 341. PMID: 30974898. PMCID: PMC6521228. DOI: 10.3390/v11040341.