缺氧诱导因子1α、Bcl-2/腺病毒E1B19kDa相互作用蛋白3在儿童创伤性脑损伤中的表达及意义

doi:10.7499/j.issn.1008-8830.2310067

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Abstract Objective To dynamically observe the changes in hypoxia-inducible factor 1α (HIF-1α) and Bcl-2/adenovirus E1B19kDa-interacting protein 3 (BNIP3) in children with traumatic brain injury (TBI) and evaluate their clinical value in predicting the severity and prognosis of pediatric TBI. Methods A prospective study included 47 children with moderate to severe TBI from January 2021 to July 2023, categorized into moderate (scores 9-12) and severe (scores 3-8) subgroups based on the Glasgow Coma Scale. A control group consisted of 30 children diagnosed and treated for inguinal hernia during the same period, with no underlying diseases. The levels of HIF-1α, BNIP3, autophagy-related protein Beclin-1, and S100B were compared among groups. The predictive value of HIF-1α, BNIP3, Beclin-1, and S100B for the severity and prognosis of TBI was assessed using receiver operating characteristic (ROC) curves. Results Serum levels of HIF-1α, BNIP3, Beclin-1, and S100B in the TBI group were higher than those in the control group (P<0.05). Among the TBI patients, the severe subgroup had higher levels of HIF-1α, BNIP3, Beclin-1, and S100B than the moderate subgroup (P<0.05). Correlation analysis showed that the serum levels of HIF-1α, BNIP3, Beclin-1, and S100B were negatively correlated with the Glasgow Coma Scale scores (P<0.05). After 7 days of treatment, serum levels of HIF-1α, BNIP3, Beclin-1, and S100B in both non-surgical and surgical TBI patients decreased compared to before treatment (P<0.05). ROC curve analysis indicated that the areas under the curve for predicting severe TBI based on serum levels of HIF-1α, BNIP3, Beclin-1, and S100B were 0.782, 0.835, 0.872, and 0.880, respectively (P<0.05), and for predicting poor prognosis of TBI were 0.749, 0.775, 0.814, and 0.751, respectively (P<0.05). Conclusions Serum levels of HIF-1α, BNIP3, and Beclin-1 are significantly elevated in children with TBI, and their measurement can aid in the clinical assessment of the severity and prognosis of pediatric TBI.

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	ZHU Lei
	WANG Xue-Cheng
	XU Yan-Yan
	WANG Nan
	ZHU Bing-Xin
	LI Zheng-Wei

Key words： Traumatic brain injury Bcl-2/adenovirus E1B19kDa-interacting protein 3 Beclin-1 Hypoxia-inducible factor 1α Child

Received: 18 October 2023

About author:: Email：xuzhouzhulei317@163.com；

Cite this article:

ZHU Lei,WANG Xue-Cheng,XU Yan-Yan et al. Expression and significance of hypoxia-inducible factor 1α and Bcl-2/adenovirus E1B19kDa-interacting protein 3 in children with traumatic brain injury[J]. CJCP, 2024, 26(4): 378-384.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2310067 OR http://www.zgddek.com/EN/Y2024/V26/I4/378

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