OBJECTIVE: The increase of oxygen-derived free radicals (OFR) or angiotensin Ⅱ(Ang Ⅱ ) in blood is one of the important causative agents of myocardium lesions. To develop a remedy for treatment of myocardium lesions induced by OFR or Ang Ⅱ , this study aims at exploring the protective effects of shenmai injection (SMI), growth hormone (GH), zinc sulfate (ZnSO4) and vitamin C (Vit C) on the cardiac myocytes injured by hydrogen peroxide (H2O2) or Ang Ⅱ. METHODS: H2O2 with the concentrations of 250 and 500 μmol/L or Ang Ⅱ with the concentrations of 10 6 and 10 7 mol/L were added respectively to the cardiac myocytes of neonatal rats so as to reduce the cardiac myocytes viability ( CMV). The injured cardica myocytes were administrated respectively with 5 ml/L and 10 ml/L (concentration) of SMI, 50 mg/L of Vit C, 15μmol/L and 60 μmol/L of ZnSO4, and 250 μmol/L and 500 μmol/L of GH. The increasing rate of CMV was measured at 24 h after medication. RESULTS: SMI, GH, ZnSO4 and VitC could all significantly elevate the increasing rate of CMV which had been decreased by H2O2 injury. The best effect was made by the SMI 10 ml/L group, followed in turn the ZnSO4 60 μmol/L, SMI 5 ml/L, Vit C 50 mg/L, GH 500 μmol/L, 15 μmol/L ZnSO4 and 250 μmol/L GH groups. There was no significant difference between the SMI 5 ml/L group and VitC 50 mg/L group. Nor was there among the GH 500 μmol/L, GH 250 μmol/L and ZnSO4 15 μmol/L groups. The CMV which had been decreased by Ang Ⅱ injury was significantly increased after administrating SMI and GH. The best effect was made by the SMI 10 ml/L group, followed in turn the SMI 5 ml/L, GH 500 μmol/L and GH 250 μmol/L groups. No difference was found among the last two groups. CONCLUSIONS: SMI, GH, ZnSO4 and VitC could all increase CMV which had been decreased by H2O2 injury, and both SMI and GH could increase CMV which had been decreased by Ang Ⅱ injury."/>
Protective Effects of Shenmai Injection, Growth Hormone, Zinc Sulfate and Vitamin C on Cardiac Myocytes Viability
Abstract OBJECTIVE: The increase of oxygen-derived free radicals (OFR) or angiotensin Ⅱ(Ang Ⅱ ) in blood is one of the important causative agents of myocardium lesions. To develop a remedy for treatment of myocardium lesions induced by OFR or Ang Ⅱ , this study aims at exploring the protective effects of shenmai injection (SMI), growth hormone (GH), zinc sulfate (ZnSO4) and vitamin C (Vit C) on the cardiac myocytes injured by hydrogen peroxide (H2O2) or Ang Ⅱ. METHODS: H2O2 with the concentrations of 250 and 500 μmol/L or Ang Ⅱ with the concentrations of 10 6 and 10 7 mol/L were added respectively to the cardiac myocytes of neonatal rats so as to reduce the cardiac myocytes viability ( CMV). The injured cardica myocytes were administrated respectively with 5 ml/L and 10 ml/L (concentration) of SMI, 50 mg/L of Vit C, 15μmol/L and 60 μmol/L of ZnSO4, and 250 μmol/L and 500 μmol/L of GH. The increasing rate of CMV was measured at 24 h after medication. RESULTS: SMI, GH, ZnSO4 and VitC could all significantly elevate the increasing rate of CMV which had been decreased by H2O2 injury. The best effect was made by the SMI 10 ml/L group, followed in turn the ZnSO4 60 μmol/L, SMI 5 ml/L, Vit C 50 mg/L, GH 500 μmol/L, 15 μmol/L ZnSO4 and 250 μmol/L GH groups. There was no significant difference between the SMI 5 ml/L group and VitC 50 mg/L group. Nor was there among the GH 500 μmol/L, GH 250 μmol/L and ZnSO4 15 μmol/L groups. The CMV which had been decreased by Ang Ⅱ injury was significantly increased after administrating SMI and GH. The best effect was made by the SMI 10 ml/L group, followed in turn the SMI 5 ml/L, GH 500 μmol/L and GH 250 μmol/L groups. No difference was found among the last two groups. CONCLUSIONS: SMI, GH, ZnSO4 and VitC could all increase CMV which had been decreased by H2O2 injury, and both SMI and GH could increase CMV which had been decreased by Ang Ⅱ injury.
MA Pei-Ran,WANG Han-Sen,LI Gang. Protective Effects of Shenmai Injection, Growth Hormone, Zinc Sulfate and Vitamin C on Cardiac Myocytes Viability[J]. 中国当代儿科杂志, 2003, 5(5): 443-446.
MA Pei-Ran,WANG Han-Sen,LI Gang. Protective Effects of Shenmai Injection, Growth Hormone, Zinc Sulfate and Vitamin C on Cardiac Myocytes Viability[J]. CJCP, 2003, 5(5): 443-446.
